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题名: E2fl1 is a meiosis-specific transcription factor in the protist Tetrahymena thermophila
作者: Zhang, Jing1, 2; Tian, Miao1, 3, 4; Yan, Guan-xiong1, 2; Shodhan, Anura3, 4; Miao, Wei1
关键词: chromosome segregation ; DNA repair ; meiosis ; Tetrahymena thermophila ; transcription factor
刊名: CELL CYCLE
发表日期: 2017
DOI: 10.1080/15384101.2016.1259779
卷: 16, 期:1, 页:123-135
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
英文摘要: Members of the E2F family of transcription factors have been reported to regulate the expression of genes involved in cell cycle control, DNA replication, and DNA repair in multicellular eukaryotes. Here, E2FL1, a meiosis-specific E2F transcription factor gene, was identified in the model ciliate Tetrahymena thermophila. Loss of this gene resulted in meiotic arrest prior to anaphase I. The cytological experiments revealed that the meiotic homologous pairing was not affected in the absence of E2FL1, but the paired homologous chromosomes did not separate and assumed a peculiar tandem arrangement. This is the first time that an E2F family member has been shown to regulate meiotic events. Moreover, BrdU incorporation showed that DSB processing during meiosis was abnormal upon the deletion of E2FL1. Transcriptome sequencing analysis revealed that E2FL1 knockout decreased the expression of genes involved in DNA replication and DNA repair in T. thermophila, suggesting that the function of E2F is highly conserved in eukaryotes. In addition, E2FL1 deletion inhibited the expression of related homologous chromosome segregation genes in T. thermophila. The result may explain the meiotic arrest phenotype at anaphase I. Finally, by searching for E2F DNA-binding motifs in the entire T. thermophila genome, we identified 714 genes containing at least one E2F DNA-binding motif; of these, 235 downregulated represent putative E2FL1 target genes.
关键词[WOS]: CELL-CYCLE CONTROL ; GENOME-WIDE IDENTIFICATION ; DNA-DAMAGE ; GENE-EXPRESSION ; NUCLEAR-LOCALIZATION ; GAMMA-TUBULIN ; TARGET GENES ; RNA-SEQ ; PROGRESSION ; FAMILY
语种: 英语
项目资助者: Projects of International Cooperation and Exchanges Ministry of Science and Technology of China(2013DFG32390) ; Natural Science Foundation of China(31525021) ; OEAD(CN10/2013) ; Austrian Science Fund(P27313-B20 ; W1238-B20)
WOS记录号: WOS:000392737300022
ISSN号: 1538-4101
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/28880
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Univ Vienna, Dept Chromosome Biol, Ctr Mol Biol, Vienna, Austria
4.Univ Vienna, Max F Perutz Labs, Ctr Mol Biol, Vienna, Austria
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