Science & Technology
; Life Sciences & Biomedicine
Gut microbiota typically occupy habitats with definable limits/borders that are comparable to oceanic islands. The gut therefore can be regarded as an 'island' for the assembly of microbial communities within the 'sea' of surrounding environments. This study aims to reveal the ecological mechanisms that govern microbiota in the fish gut 'island' ecosystem. Taxonomic compositions, phylogenetic diversity, and community turnover across host development were analyzed via the high-throughput sequencing of 16S rRNA gene amplicons. The results indicate that the Shannon diversity of gut microbiota in the three examined freshwater fish species all significantly decreased with host development, and the dominant bacterial taxa also changed significantly during host development. Null model and phylogenetic-based mean nearest taxon distance (MNTD) analyses suggest that host gut environmental filtering led to the assembly of microbial communities in the fish gut 'island'. However, the phylogenetic clustering of local communities and deterministic processes that governed community turnover became less distinct as the fish developed. The observed mechanisms that shaped fish gut microbiota seemed to be mainly shaped by the gut environment and by some other selective changes accompanying the host development process. These findings greatly enhance our understanding of stage-specific community assembly patterns in the fish gut ecosystem.
National Natural Science Foundation of China(31400109
; Youth Innovation Promotion Association of the Chinese Academy of Sciences(Y22Z07)
; Office of the Vice President for Research at the University of Oklahoma
1.Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China 3.Qilu Normal Univ, Jinan 250013, Peoples R China 4.Univ Oklahoma, Inst Environm Genom, Norman, OK 73019 USA 5.Univ Oklahoma, Dept Microbiol & Plant Biol, Norman, OK 73019 USA 6.Chinese Acad Sci, State Key Lab Lake Sci & Environm, Nanjing Inst Geog & Limnol, Nanjing 210008, Peoples R China 7.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 8.Guangdong Inst Microbiol, State Key Lab Appl Microbiol Southern China, Guangzhou 510070, Guangdong, Peoples R China 9.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 10.Tsinghua Univ, State Key Joint Lab Environm Simulat & Pollut Con, Sch Environm, Beijing 100084, Peoples R China 11.Lawrence Berkeley Natl Lab, Div Earth Sci, Berkeley, CA 94720 USA