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题名: Responses of the Proteome and Metabolome in Livers of Zebrafish Exposed Chronically to Environmentally Relevant Concentrations of Microcystin-LR
作者: Chen, Liang1, 2; Hu, Yufei1, 2; He, Jun1; Chen, Jun1; Giesy, John P.3, 4, 5, 6; Xie, Ping1
刊名: ENVIRONMENTAL SCIENCE & TECHNOLOGY
发表日期: 2017-01-03
DOI: 10.1021/acs.est.6b03990
卷: 51, 期:1, 页:596-607
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Technology ; Life Sciences & Biomedicine
类目[WOS]: Engineering, Environmental ; Environmental Sciences
研究领域[WOS]: Engineering ; Environmental Sciences & Ecology
英文摘要: In this study, for the first time, changes in expressions of proteins and profiles of metabolites in liver of the small, freshwater fish Danio rerio (zebrafish) were investigated after long-term exposure to environmentally relevant concentrations of microcystin-LR (MC-LR). Male zebrafish were exposed via water to 1 or 10 mu g MC-LR/L for 90 days, and iTRAQ-based proteomics and H-1 NMR-based metabolomics were employed. Histopathological observations showed that MC-LR caused damage to liver, and the effects were more pronounced in fish exposed to 10 mu g MC-LR/L. Metabolomic analysis also showed alterations of hepatic function, which included changes in a number of metabolic pathways, including small molecules involved in energy, glucose, lipids, and amino acids metabolism. Concentrations of lactate were significantly greater in individuals exposed to MC-LR than in unexposed controls. This indicated a shift toward anaerobic metabolism, which was confirmed by impaired respiration in mitochondria. Proteomics revealed that MC-LR significantly influenced multiple proteins, including those involved in folding of proteins and metabolism. Endoplasmic reticulum stress contributed to disturbance of metabolism of lipids in liver of zebrafish exposed to MC-LR. Identification of proteins and metabolites in liver of zebrafish responsive to MC-LR provides insights into mechanisms of chronic toxicity of MCs.
关键词[WOS]: ENDOPLASMIC-RETICULUM STRESS ; FISH ORYZIAS-LATIPES ; NF-KAPPA-B ; OXIDATIVE STRESS ; DANIO-RERIO ; MEDAKA FISH ; MOLECULAR-MECHANISMS ; 1ST IDENTIFICATION ; INDUCED APOPTOSIS ; AQUATIC ANIMALS
语种: 英语
项目资助者: Excellent Young Scientists Fund ; National Natural Science Foundation of China(31322013) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ08) ; "High Level Foreign Experts" program - State Administration of Foreign Experts Affairs, China(GDT20143200016) ; Canada Research Chair program ; Distinguished Visiting Professorship in School of Biological Sciences, University of Hong Kong
WOS记录号: WOS:000391346900066
ISSN号: 0013-936X
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/28861
Appears in Collections:淡水生态学研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Univ Saskatchewan, Dept Vet Biomed Sci, Saskatoon, SK S7N 5B3, Canada
4.Univ Saskatchewan, Toxicol Ctr, Saskatoon, SK S7N 5B3, Canada
5.Univ Hong Kong, Sch Biol Sci, Hong Kong, Hong Kong, Peoples R China
6.Nanjing Univ, Sch Environm, State Key Lab Pollut Control & Resource Reuse, Nanjing 210089, Jiangsu, Peoples R China
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