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题名: Analysis of genetic variation in the Chinese sturgeon, Acipenser sinensis: estimating the contribution of artificially produced larvae in a wild population
作者: Zhu, B; Zhou, F; Cao, H; Shao, Z; Zhao, N; May, B; Chang, J
刊名: JOURNAL OF APPLIED ICHTHYOLOGY
发表日期: 2002-12-01
卷: 18, 期:4-6, 页:301-306
收录类别: ISTP ; SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Fisheries ; Marine & Freshwater Biology
研究领域[WOS]: Fisheries ; Marine & Freshwater Biology
英文摘要: Twenty-five sets of microsatellite primers developed from lake sturgeon ( Acipenser fulvescens) and shovelnose sturgeon (Scaphirhynchus platorynchus) genomic DNA were tested on Chinese sturgeon, Acipenser sinensis. Ten sets of primers successfully produced resolvable amplicons, and four of these sets ( Afu-39, Afu-54, Afu-68, and Spl-168) were used to analyze genetic variation in mature adults, juveniles, and single family of Chinese sturgeon from the Yangtze River. Offspring from the single family were stocked prior to the juvenile sample being taken. Results from genotyping parents and offspring indicated that all four loci appear to be tetrasomic and all alleles appear to segregate among the offspring in 1 : 1 ( presence : absence) ratio. Neighbor-joining based on band-sharing in coupling with parentage analysis revealed that hatchery propagated individuals may comprise 5-10% of the juvenile population from stocking of 30 000-60 000 larvae.
关键词[WOS]: MICROSATELLITE LOCI ; (GT)(N) MICROSATELLITES ; DNA ; MARKERS ; SCAPHIRHYNCHUS ; VARIABILITY ; INHERITANCE ; SEQUENCES
语种: 英语
WOS记录号: WOS:000179913400012
ISSN号: 0175-8659
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/28828
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Univ Calif Davis, Dept Anim Sci, Davis, CA 95616 USA
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