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Transcriptome analysis of grass carp provides insights into disease-related genes and novel regulation pattern of bile acid feedback in response to lithocholic acid
Xiong, Fan1,2; Wu, Shangong1,2; Qin, Lu1,2; Shi, Mijuan1; Li, Wenxiang1,2; Zou, Hong1; Li, Ming1,2; Wang, Guitang1,2
2019-02-01
Source PublicationAQUACULTURE
ISSN0044-8486
Volume500Issue:1Pages:613-621
Abstract

Gut microbiota and its metabolites affect lipid metabolism and liver health. Grass carp (Ctenopharyngodon idellus) often suffers from functional disorders of liver and gallbladder, which is usually accompanied by accumulation of lipids in the liver. Here we studied liver transcriptome and microbial fluctuation in the gut in response to dietary lithocholic acid (LCA; a metabolite of gut microbiota) supplementation in grass carp. A total of 1802 differentially expressed genes (DEGs) were identified between LCA and Control groups according to the liver transcriptome analysis. Of these DEGs, 8 nonalcoholic fatty liver disease-related genes were all upregulated, and 11 of 12 cancer related genes were upregulated in the LCA group. We also found significantly upregulated bile acid receptor, hepatocyte nuclear factor 4-beta (HNF4B), in the LCA group. The red oil O staining of liver showed a higher abundance of lipid droplets in the LCA group. LCA also changed the composition of gut microbiota, with increasing proportion of Proteobacteria and Firmicutes, whereas Fusobacteria decreased. Co-occurrence between DEGs and microbial taxa was mainly identified between signal transduction and genetic information processing-related genes and Proteobacteria and Firmicutes taxa. Overall, our results elucidate the effects of LCA on liver and gut microbiota in fish, and suggest a novel pattern of bile acid feedback in grass carp. These results contribute to our understanding of the interplay between gut microbiota and liver diseases in fish, and offer insights into putative treatments of liver diseases in grass carp.

KeywordBile acid Gut microbiota Lipid metabolism Nonalcoholic fatty liver disease Freshwater fish
DOI10.1016/j.aquaculture.2018.10.015
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31872612] ; earmarked fund for China Agriculture Research System[CARS-45-15]
WOS Research AreaFisheries ; Marine & Freshwater Biology
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS IDWOS:000452969500075
WOS KeywordGUT MICROBIOTA ; SALT BIOTRANSFORMATIONS ; SIGNALING PATHWAY ; NUCLEAR RECEPTOR ; SP NOV. ; EVOLUTION ; STRINGTIE ; PROTEIN ; GENOME ; CANCER
PublisherELSEVIER SCIENCE BV
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/28821
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorWu, Shangong
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquaculture Dis Control, State Key Lab Freshwater Ecol & Biotechnol,Minist, Wuhan, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Xiong, Fan,Wu, Shangong,Qin, Lu,et al. Transcriptome analysis of grass carp provides insights into disease-related genes and novel regulation pattern of bile acid feedback in response to lithocholic acid[J]. AQUACULTURE,2019,500(1):613-621.
APA Xiong, Fan.,Wu, Shangong.,Qin, Lu.,Shi, Mijuan.,Li, Wenxiang.,...&Wang, Guitang.(2019).Transcriptome analysis of grass carp provides insights into disease-related genes and novel regulation pattern of bile acid feedback in response to lithocholic acid.AQUACULTURE,500(1),613-621.
MLA Xiong, Fan,et al."Transcriptome analysis of grass carp provides insights into disease-related genes and novel regulation pattern of bile acid feedback in response to lithocholic acid".AQUACULTURE 500.1(2019):613-621.
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