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DNA Barcoding of Catfish: Species Authentication and Phylogenetic Assessment
Wong, Li Lian1; Peatman, Eric1; Lu, Jianguo1; Kucuktas, Huseyin1; He, Shunping2; Zhou, Chuanjiang2,3; Na-nakorn, Uthairat4; Liu, Zhanjiang1
Source PublicationPLOS ONE
AbstractAs the global market for fisheries and aquaculture products expands, mislabeling of these products has become a growing concern in the food safety arena. Molecular species identification techniques hold the potential for rapid, accurate assessment of proper labeling. Here we developed and evaluated DNA barcodes for use in differentiating United States domestic and imported catfish species. First, we sequenced 651 base-pair barcodes from the cytochrome oxidase I (COI) gene from individuals of 9 species (and an Ictalurid hybrid) of domestic and imported catfish in accordance with standard DNA barcoding protocols. These included domestic Ictalurid catfish, and representative imported species from the families of Clariidae and Pangasiidae. Alignment of individual sequences from within a given species revealed highly consistent barcodes (98% similarity on average). These alignments allowed the development and analyses of consensus barcode sequences for each species and comparison with limited sequences in public databases (GenBank and Barcode of Life Data Systems). Validation tests carried out in blinded studies and with commercially purchased catfish samples (both frozen and fresh) revealed the reliability of DNA barcoding for differentiating between these catfish species. The developed protocols and consensus barcodes are valuable resources as increasing market and governmental scrutiny is placed on catfish and other fisheries and aquaculture products labeling in the United States.
WOS HeadingsScience & Technology
Indexed BySCI
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000288513900034
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Document Type期刊论文
Affiliation1.Auburn Univ, Dept Fisheries & Allied Aquacultures, Aquat Genom Unit, Auburn, AL 36849 USA
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Hubei, Peoples R China
3.Southwest Univ, Sch Life Sci, Chongqing, Peoples R China
4.Kasetsart Univ, Fac Fisheries, Dept Aquaculture, Bangkok, Thailand
Recommended Citation
GB/T 7714
Wong, Li Lian,Peatman, Eric,Lu, Jianguo,et al. DNA Barcoding of Catfish: Species Authentication and Phylogenetic Assessment[J]. PLOS ONE,2011,6(3).
APA Wong, Li Lian.,Peatman, Eric.,Lu, Jianguo.,Kucuktas, Huseyin.,He, Shunping.,...&Liu, Zhanjiang.(2011).DNA Barcoding of Catfish: Species Authentication and Phylogenetic Assessment.PLOS ONE,6(3).
MLA Wong, Li Lian,et al."DNA Barcoding of Catfish: Species Authentication and Phylogenetic Assessment".PLOS ONE 6.3(2011).
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