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题名: Full-length normalization subtractive hybridization analysis provides new insights into sexual precocity and ovarian development of red swamp crayfish Procambarus clarkii
作者: Tan, Hong-Yue1; Shao, Guang-Ming1, 2; Kang, Peng-Fei1; Wang, Yu-Feng1
关键词: Procambarus clarkii ; Sexual precocity ; C1q-like ; Ovarian development
刊名: AQUACULTURE
发表日期: 2017-02-01
DOI: 10.1016/j.aquaculture.2016.10.043
卷: 468, 页:417-427
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Fisheries ; Marine & Freshwater Biology
研究领域[WOS]: Fisheries ; Marine & Freshwater Biology
英文摘要: The red swamp crayfish Procambarus clarkii is an important freshwater economic crayfish in Chinese inland due to its high market value and consumer demand. The sexual precocity in this species has received considerable attention in recent years because more and more individuals matured at small sizes, which devalues the commercial production. In this study, by the full-length normalization subtractive hybridization (FNSH) method we identified 16 differently expressed sequences between precocious and normal grown P. clarkii ovaries. Of the down-regulated sequences in precocious ovaries, one encodes a peptide which shared the highest identity to C1q-like family and contained a typical C1q domain at the C-terminal, thus it was named PcC1q-like. QRT-PCR analysis showed that PcC1q-like was transcribed specifically in ovary, with higher expression level in vitellogenic stages. In situ hybridization exhibited specific expression of PcC1q-like in follicular cells around the vitellogenic oocytes. Knockdown of PcC1q-like by injection of double strand RNA (dsRNA) resulted in disruption of follicular cells, decreased transcripts of vitellogenin (Vg) and vitellogenin receptor (VgR), and reduced progesterone content. These results suggest that PcC1q-like may promote ovarian development by stimulating the synthesis and transportation of vitellogenin into oocytes. These data provide insights into the molecular regulatory mechanisms of sexual precocity and ovarian development in crustaceans.
关键词[WOS]: TUMOR-NECROSIS-FACTOR ; CRAB ERIOCHEIR-SINENSIS ; GIANT TIGER SHRIMP ; C1Q FAMILY-MEMBER ; MOLECULAR CHARACTERIZATION ; MACROBRACHIUM-NIPPONENSE ; PENAEUS-JAPONICUS ; KURUMA PRAWN ; VITELLOGENIN ; EXPRESSION
语种: 英语
项目资助者: Program for Academic Leader in Wuhan city(201051730554)
WOS记录号: WOS:000390735200054
ISSN号: 0044-8486
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/28764
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
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