Zebrafish STAT6 negatively regulates IFN phi 1 production by attenuating the kinase activity of TANK-binding kinase 1
Li, Shun1; Lu, Long-Feng1,2; LaPatra, Scott E.3; Chen, Dan-Dan1; Zhang, Yong-An1
2017-02-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
Volume67Pages:189-201
AbstractThe aquatic spring viremia of carp virus (SVCV) causes significant mortality in common carp (Cyprinus carpio), and TBK1 plays a crucial role in the retinoic acid-inducible gene I (RIG-I)-like receptor (RLR) system by phosphorylating its substrates to induce type I interferons (IFNs) and cellular antiviral responses. In this study, we report that zebrafish STAT6 is induced during SVCV infection and reduces IFN phi 1 expression by suppressing TBK1 phosphorylation. A typical IFN stimulatory response element (ISRE) motif was found in the promoter region of zebrafish STAT6, and zebrafish STAT6 transcription was significantly upregulated in the early stages of virus infection. Overexpression of STAT6 interfered with IFN phi 1 promoter activity in response to SVCV infection. Additionally, TBK1-, but not MITA-mediated activation of the IFN phi 1 promoter was impaired by STAT6. Co-immunoprecipitation and Western blot experiments indicated that MITA and IRF3 were significantly phosphorylated by TBK1, and that the N-terminal kinase domain of TBK1 was critical in this process. In the final step, STAT6 interacted with the N-terminal kinase domain of TBK1 causing dephosphorylation, which resulted in reductions in the phosphorylation of IRF3 and the production of IFN phi 1. These results indicate that fish STAT6 can attenuate the kinase activity of TBK1, leading to suppression of IFN phi 1 expression which may in turn facilitate virus replication. (C) 2016 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordStat6 Negative Regulator Interferon Tbk1 Zebrafish
DOI10.1016/j.dci.2016.10.003
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNational Basic Research Program of China(2014CB138601) ; National Basic Research Program of China(2014CB138601) ; National Natural Science Foundation of China(31502200) ; National Natural Science Foundation of China(31502200) ; Knowledge Innovation Program of the Chinese Academy of Sciences(Y65E01-1-501) ; Knowledge Innovation Program of the Chinese Academy of Sciences(Y65E01-1-501) ; National Basic Research Program of China(2014CB138601) ; National Basic Research Program of China(2014CB138601) ; National Natural Science Foundation of China(31502200) ; National Natural Science Foundation of China(31502200) ; Knowledge Innovation Program of the Chinese Academy of Sciences(Y65E01-1-501) ; Knowledge Innovation Program of the Chinese Academy of Sciences(Y65E01-1-501)
Language英语
WOS Research AreaImmunology ; Zoology
WOS SubjectImmunology ; Zoology
WOS KeywordINNATE IMMUNE-RESPONSE ; I-INTERFERON ; ANTIVIRAL RESPONSE ; FISH ; ACTIVATION ; TRANSCRIPTION ; INDUCTION ; TBK1 ; IRF3 ; CELL
WOS IDWOS:000390071200018
Funding OrganizationNational Basic Research Program of China(2014CB138601) ; National Basic Research Program of China(2014CB138601) ; National Natural Science Foundation of China(31502200) ; National Natural Science Foundation of China(31502200) ; Knowledge Innovation Program of the Chinese Academy of Sciences(Y65E01-1-501) ; Knowledge Innovation Program of the Chinese Academy of Sciences(Y65E01-1-501) ; National Basic Research Program of China(2014CB138601) ; National Basic Research Program of China(2014CB138601) ; National Natural Science Foundation of China(31502200) ; National Natural Science Foundation of China(31502200) ; Knowledge Innovation Program of the Chinese Academy of Sciences(Y65E01-1-501) ; Knowledge Innovation Program of the Chinese Academy of Sciences(Y65E01-1-501)
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/28758
Collection藻类生物学及应用研究中心_水生生物分子与细胞生物学研究中心
Affiliation1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Clear Spring Foods Inc, Div Res, Buhl, ID 83316 USA
Recommended Citation
GB/T 7714
Li, Shun,Lu, Long-Feng,LaPatra, Scott E.,et al. Zebrafish STAT6 negatively regulates IFN phi 1 production by attenuating the kinase activity of TANK-binding kinase 1[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2017,67:189-201.
APA Li, Shun,Lu, Long-Feng,LaPatra, Scott E.,Chen, Dan-Dan,&Zhang, Yong-An.(2017).Zebrafish STAT6 negatively regulates IFN phi 1 production by attenuating the kinase activity of TANK-binding kinase 1.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,67,189-201.
MLA Li, Shun,et al."Zebrafish STAT6 negatively regulates IFN phi 1 production by attenuating the kinase activity of TANK-binding kinase 1".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 67(2017):189-201.
Files in This Item:
File Name/Size DocType Version Access License
1-s2.0-S0145305X1630(2210KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Li, Shun]'s Articles
[Lu, Long-Feng]'s Articles
[LaPatra, Scott E.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Li, Shun]'s Articles
[Lu, Long-Feng]'s Articles
[LaPatra, Scott E.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Li, Shun]'s Articles
[Lu, Long-Feng]'s Articles
[LaPatra, Scott E.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 1-s2.0-S0145305X16303342-main.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.