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题名: Characterization, optimization, and validation of environmental DNA (eDNA) markers to detect an endangered aquatic mammal
作者: Ma, Hongjuan1; Stewart, Kathryn1; Lougheed, Stephen2; Zheng, Jinsong3; Wang, Yuxiang2; Zhao, Jianfu1
关键词: Environmental DNA ; eDNA ; Primers ; Detection sensitivity ; Detection longevity ; Amplicon length ; Conservation management ; Yangtze finless porpoise ; Neophocaena asiaeorientalis asiaeorientalis
刊名: CONSERVATION GENETICS RESOURCES
发表日期: 2016-12-01
DOI: 10.1007/s12686-016-0597-9
卷: 8, 期:4, 页:561-568
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biodiversity Conservation ; Genetics & Heredity
研究领域[WOS]: Biodiversity & Conservation ; Genetics & Heredity
英文摘要: Environmental DNA (eDNA) represents a sensitive and efficient method for noninvasively sampling rare or otherwise hard to monitor taxa, potentially making it a powerful tool for conservation management. Still, this novel method can be affected by sampling protocols, abiotic characteristics of the microhabitat, the focal taxa itself, and primer design. Here we designed 12 species-specific primers for the critically endangered Yangtze finless porpoise (Neophocaena asiaeorientalis asiaeorientalis) inhabiting the middle to lower Yangtze River in China to enable complementary population survey tools for conservation efforts. Representing primer pairs that amplify a range of DNA fragment sizes, we test these primers for sensitivity at amplifying finless porpoise DNA using conventional PCR from serial diluted blood samples, and from eDNA in aquaria and in the wild, including a nature reserve and a negative control site. We further investigated the capacity for these primers to detect finless porpoise DNA signals from water samples over a 30-day period. Our study presents which primers were successful at amplifying finless porpoise DNA from aquaria and in the wild, and further demonstrates no significant amplicon size effects on primer sensitivity or longevity. We summarize the impact primer design may have on eDNA applications in general and suggest future considerations for conservation efforts with the Yangtze finless porpoise.
关键词[WOS]: YANGTZE FINLESS PORPOISE ; CONSERVATION ; PERSISTENCE ; TOOL ; PCR
语种: 英语
项目资助者: National Natural Sciences Foundation of China (NSFC) ; Tongji University State Key Laboratory for Pollution Control and Resource Reuse
WOS记录号: WOS:000387482700037
ISSN号: 1877-7252
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/28688
Appears in Collections:水生生物多样性与资源保护研究中心_期刊论文

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作者单位: 1.Tongji Univ, Coll Environm Sci & Engn, Shanghai, Peoples R China
2.Queens Univ, Dept Biol, Kingston, ON, Canada
3.Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan, Peoples R China
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