IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Identification, characterisation and preliminary functional analysis of IRAK-M in grass carp (Ctenopharyngodon idella)
Chu, Pengfei1,2; He, Libo1; Zhu, Denghui1,2; Chen, Liangming1,2; Huang, Rong1; Liao, Lanjie1; Li, Yongming1; Zhu, Zuoyan1; Wang, Yaping1
Corresponding AuthorWang, Yaping(wangyp@ihb.ac.cn)
2019
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume84Pages:312-321
AbstractInterleukin-1 receptor-associated kinase (IRAK) family members play important roles in myeloid differentiation primary response 88 (MyD88)-dependent toll-like receptor (TLR) signaling, the crucial innate immune pathway in vertebrates. In the present study, the IRAK family gene IRAK-M (also called IRAK3) from grass carp (Ctenopharyngodon idella) was cloned and characterised. IRAK-M was mainly enriched in the spleen, and the significantly altered expression was observed after grass carp reovirus (GCRV) infection. Subcellular localisation showed that IRAK-M protein distributed uniformly in the entire cell and co-localised with MyD88 in the cytoplasm of transfected cells. Additionally, the interaction between IRAK-M and MyD88 was confirmed by bimolecular fluorescence complementation (BiFC) system. Moreover, deficient of IRAK-M in C. idella kidney cell line (CIK) with small interference RNA (siRNA) upregulated polyinosinic:polycytidylic acid (poly(I:C))-induced inflammatory cytokines production, including interleukin 8 (1L-8), 1L-6, and tumour necrosis factor alpha (TNF-alpha), which reveals that IRAK-M functions as a negative regulator of inflammatory cytokines. Taken together, our results demonstrate that IRAK-M gene plays an important role in innate immune regulation and provide new insights into understanding the functional characteristics of the IRAK-M in teleosts.
KeywordGrass carp IRAK-M Innate immune Subcellular localisation MyD88
DOI10.1016/j.fsi.2018.09.080
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences
Indexed BySCI ; SCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31721005] ; National Natural Science Foundation of China[31702332] ; Hubei Natural Science Foundation of China[2015CFA007] ; Hubei Natural Science Foundation of China[2017CFB374] ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences[XDA08030203]
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000457666800036
WOS KeywordNF-KAPPA-B ; TOLL-LIKE RECEPTORS ; SIGNALING PATHWAYS ; GENE-EXPRESSION ; UP-REGULATION ; ACTIVATION ; AUTOPHOSPHORYLATION ; MACROPHAGES ; MOLECULES ; PROMOTES
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/28620
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorWang, Yaping
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Chu, Pengfei,He, Libo,Zhu, Denghui,et al. Identification, characterisation and preliminary functional analysis of IRAK-M in grass carp (Ctenopharyngodon idella)[J]. FISH & SHELLFISH IMMUNOLOGY,2019,84:312-321.
APA Chu, Pengfei.,He, Libo.,Zhu, Denghui.,Chen, Liangming.,Huang, Rong.,...&Wang, Yaping.(2019).Identification, characterisation and preliminary functional analysis of IRAK-M in grass carp (Ctenopharyngodon idella).FISH & SHELLFISH IMMUNOLOGY,84,312-321.
MLA Chu, Pengfei,et al."Identification, characterisation and preliminary functional analysis of IRAK-M in grass carp (Ctenopharyngodon idella)".FISH & SHELLFISH IMMUNOLOGY 84(2019):312-321.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Chu, Pengfei]'s Articles
[He, Libo]'s Articles
[Zhu, Denghui]'s Articles
Baidu academic
Similar articles in Baidu academic
[Chu, Pengfei]'s Articles
[He, Libo]'s Articles
[Zhu, Denghui]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Chu, Pengfei]'s Articles
[He, Libo]'s Articles
[Zhu, Denghui]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.