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High level of reactive oxygen species inhibits triacylglycerols accumulation in Chlamydomonas reinhardtii
Miao, Rongli1,2; Ma, Xiaocui1,2; Deng, Xuan1; Huang, Kaiyao1
Corresponding AuthorHuang, Kaiyao(huangky@ihb.ac.cn)
2019-03-01
Source PublicationALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
ISSN2211-9264
Volume38Pages:13
AbstractMany microalgae accumulate large amounts of lipid droplets (LDs) following N deprivation. The triacylglycerols (TAGs) in these LDs can be used as a feedstock to produce biodiesel. To investigate the molecular mechanism underlying LD formation, we used a percoll gradient-based method to enrich for mutants with defects in LD formation and generated an insertional mutagenesis library containing > 11,000 Chlamydomonas reinhardtii transformants. One of the mutants harbored a mutation in a gene encoding a glutathione peroxidase (GPX5) that catalyzes the formation of water from hydrogen peroxide (H2O2) or organic hydroperoxide. The gpx5 mutant had a 35% reduction in the number of LDs and a 50% reduction in TAG content compared with the parental strain CC4348 at 24 h of N deprivation. The full-length of GPX5 or truncate GPX5 without the putative signal peptide or the putative signal peptide of GPX5 fused YFP localized in the cytoplasm, but not in chloroplasts. Complementation with full-length GPX5 rescued the mutant phenotypes, and the expression of GPX5 in a complemented strain L27 was increased 2-3 fold after 24 h of N deprivation. Moreover, the gpx5 mutant showed increased sensitivity to the singlet oxygen (O-1(2)) stress generated by the photosensitizer Rose Bengal (RB). The ROS concentration of the gpx5 was about 1.5 times that of CC4348 during N deprivation. Artificial increasing of ROS concentration in cells abolished the formation of LDs in Chlamydomonas. These data suggest that a high level of ROS attenuates LD formation.
KeywordChlamydomonas Lipid droplets (LDs) Reactive oxygen species (ROS) Glutathione peroxidase (GPX) Mutants
DOI10.1016/j.algal.2018.101400
Funding OrganizationNational High-tech R&D Program (863 Program) ; National High-tech R&D Program (863 Program) ; National High-tech R&D Program (863 Program) ; National High-tech R&D Program (863 Program) ; National High-tech R&D Program (863 Program) ; National High-tech R&D Program (863 Program) ; National High-tech R&D Program (863 Program) ; National High-tech R&D Program (863 Program) ; National High-tech R&D Program (863 Program) ; National High-tech R&D Program (863 Program) ; National High-tech R&D Program (863 Program) ; National High-tech R&D Program (863 Program) ; National High-tech R&D Program (863 Program) ; National High-tech R&D Program (863 Program) ; National High-tech R&D Program (863 Program) ; National High-tech R&D Program (863 Program)
Indexed BySCI ; SCI
Language英语
Funding ProjectNational High-tech R&D Program (863 Program)[2014AA022001]
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000458402800018
WOS KeywordPEROXIDASE HOMOLOGOUS GENE ; SINGLET OXYGEN ; NITROGEN DEPRIVATION ; LIPID-ACCUMULATION ; REVERSE GENETICS ; STRESS-RESPONSE ; MUTANT LIBRARY ; GPXH GENE ; EXPRESSION ; SYSTEMS
PublisherELSEVIER SCIENCE BV
Funding OrganizationNational High-tech R&D Program (863 Program) ; National High-tech R&D Program (863 Program) ; National High-tech R&D Program (863 Program) ; National High-tech R&D Program (863 Program) ; National High-tech R&D Program (863 Program) ; National High-tech R&D Program (863 Program) ; National High-tech R&D Program (863 Program) ; National High-tech R&D Program (863 Program) ; National High-tech R&D Program (863 Program) ; National High-tech R&D Program (863 Program) ; National High-tech R&D Program (863 Program) ; National High-tech R&D Program (863 Program) ; National High-tech R&D Program (863 Program) ; National High-tech R&D Program (863 Program) ; National High-tech R&D Program (863 Program) ; National High-tech R&D Program (863 Program)
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/28617
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorHuang, Kaiyao
Affiliation1.Chinese Acad Sci, Key Lab Algal Biol, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Miao, Rongli,Ma, Xiaocui,Deng, Xuan,et al. High level of reactive oxygen species inhibits triacylglycerols accumulation in Chlamydomonas reinhardtii[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2019,38:13.
APA Miao, Rongli,Ma, Xiaocui,Deng, Xuan,&Huang, Kaiyao.(2019).High level of reactive oxygen species inhibits triacylglycerols accumulation in Chlamydomonas reinhardtii.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,38,13.
MLA Miao, Rongli,et al."High level of reactive oxygen species inhibits triacylglycerols accumulation in Chlamydomonas reinhardtii".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 38(2019):13.
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