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题名: A New Paradigm for Producing Astaxanthin From the Unicellular Green Alga Haematococcus pluvialis
作者: Zhang, Zhen1, 2; Wang, Baobei2, 4; Hu, Qiang3; Sommerfeld, Milton2; Li, Yuanguang1; Han, Danxiang3
关键词: astaxanthin ; Haematococcus pluvialis ; heterotrophy ; acclimation
刊名: BIOTECHNOLOGY AND BIOENGINEERING
发表日期: 2016-10-01
DOI: 10.1002/bit.25976
卷: 113, 期:10, 页:2088-2099
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology
研究领域[WOS]: Biotechnology & Applied Microbiology
英文摘要: The unicellular green alga Haematococcus pluvialis has been exploited as a cell factory to produce the high-value antioxidant astaxanthin for over two decades, due to its superior ability to synthesize astaxanthin under adverse culture conditions. However, slow vegetative growth under favorable culture conditions and cell deterioration or death under stress conditions (e.g., high light, nitrogen starvation) has limited the astaxanthin production. In this study, a new paradigm that integrated heterotrophic cultivation, acclimation of heterotrophically grown cells to specific light/nutrient regimes, followed by induction of astaxanthin accumulation under photoautotrophic conditions was developed. First, the environmental conditions such as pH, carbon source, nitrogen regime, and light intensity, were optimized to induce astaxanthin accumulation in the dark-grown cells. Althoughmoderate astaxanthin content (e.g., 1% of dry weight) and astaxanthin productivity (2.5mg L-1 day(-1)) were obtained under the optimized conditions, a considerable number of cells died off when subjected to stress for astaxanthin induction. To minimize the susceptibility of dark-grown cells to light stress, the algal cells were acclimated, prior to light induction of astaxanthin biosynthesis, under moderate illumination in the presence of nitrogen. Introduction of this strategy significantly reduced the cell mortality rate under high-light and resulted in increased cellular astaxanthin content and astaxanthin productivity. The productivity of astaxanthin was further improved to 10.5mg L-1 day(-1) by implementation of such a strategy in a bubbling column photobioreactor. Biochemical and physiological analyses suggested that rebuilding of photosynthetic apparatus including D1 protein and PsbO, and recovery of PSII activities, are essential for acclimation of dark-grown cells under photo-induction conditions. (C) 2016 The Authors. Biotechnology and Bioengineering Published by Wiley Periodicals, Inc.
关键词[WOS]: DILUTION-PHOTOINDUCTION CULTIVATION ; DEPENDENT CHLOROPHYLL BIOSYNTHESIS ; CHLAMYDOMONAS-REINHARDTII ; HETEROTROPHIC GROWTH ; FLUORESCENCE ; LACUSTRIS ; CULTURES ; IDENTIFICATION ; CHLOROPLASTS ; MICROALGAE
语种: 英语
项目资助者: State Development & Investment Corporation (SDIC) of China ; National Science Foundation of Hubei Province(2015CFA022)
WOS记录号: WOS:000386753600002
ISSN号: 0006-3592
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/28607
Appears in Collections:藻类生物技术和生物能源研发中心_期刊论文

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作者单位: 1.East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China
2.Arizona State Univ, Dept Human Syst & Environm, Mesa, AZ USA
3.Chinese Acad Sci, Inst Hydrobiol, Ctr Microalgal Biotechnol & Biofuels, Wuhan, Hubei, Peoples R China
4.Xiamen Univ, Dept Chem & Biochem Engn, Xiamen, Fujian, Peoples R China
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