中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
题名: Fish IRF6 is a positive regulator of IFN expression and involved in both of the MyD88 and TBK1 pathways
作者: Li, Shun1; Lu, Long-Feng1, 2; Wang, Zhao-Xi1, 2; Chen, Dan-Dan1; Zhang, Yong-An1
关键词: IRF6 ; Positive regulator ; Interferon ; TBK1 ; MyD88
刊名: FISH & SHELLFISH IMMUNOLOGY
发表日期: 2016-10-01
DOI: 10.1016/j.fsi.2016.08.059
卷: 57, 页:262-268
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
英文摘要: Interferon (IFN) regulatory factors (IRF) are the crucial transcription factors for IFN expression, leading host cell response to viral infection. In mammals, only IRF6 is unaffected by IFN expression in the IRF family; however, in fish, a lower vertebrate, whether IRF6 is related to IFN regulation is unclear. In this study, we identified that zebrafish IRF6 was a positive regulator of IFN transcription and could be phosphorylated by both MyD88 and TBK1. First, the transcript level of cellular irf6 was upregulated by treatment with poly I:C (a mimic of viral RNAs), indicating IRF6 might be involved in the process of host cell response to viruses. Overexpression of IRF6 could upregulate IFN promoter activity significantly, meaning IRF6 is a positive regulator of IFN transcription. Subsequently, at the protein regulation level and in the interaction relationship, IRF6 was phosphorylated by and associated with both MyD88 and. TBK1. In addition, overexpression of IRF6 activated the transcription of isg15, rig-i and mavs of host cells; meanwhile, the transcripts of p, m and n genes of SVCV were significantly declined in IRF6-overexpressing cells. Taken together, our data demonstrate that fish IRF6 is distinguished from the homolog of mammals by being a positive regulator of IFN transcription and phosphorylated by MyD88 and TBK1, suggesting that differences in the IRF6 regulation pattern exist between lower and higher vertebrates. (C) 2016 Elsevier Ltd. All rights reserved.
关键词[WOS]: ANTIVIRAL SIGNALING PROTEIN ; INNATE IMMUNE-RESPONSE ; CTENOPHARYNGODON-IDELLA ; ONCORHYNCHUS-MYKISS ; RAINBOW-TROUT ; RIG-I ; CELLS ; GENE ; RNA ; RECOGNITION
语种: 英语
项目资助者: National Basic Research Program of China(2014CB138601) ; National Natural Science Foundation of China(31502200) ; Knowledge Innovation Program of the Chinese Academy of Sciences(Y65E01-1-501)
WOS记录号: WOS:000385327100028
ISSN号: 1050-4648
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/28599
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
1-s2.0-S1050464816305435-main.pdf(781KB)----开放获取--View Download

作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Li, Shun]'s Articles
[Lu, Long-Feng]'s Articles
[Wang, Zhao-Xi]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Li, Shun]‘s Articles
[Lu, Long-Feng]‘s Articles
[Wang, Zhao-Xi]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: 1-s2.0-S1050464816305435-main.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2018  中国科学院水生生物研究所 - Feedback
Powered by CSpace