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Chemical proteomic analysis of the potential toxicological mechanisms of microcystin-RR in zebrafish (Danio rerio) liver
Tuo, Xun1,2,3; Chen, Jun1; Zhao, Sujuan1,3; Xie, Ping1
2016-10-01
Source PublicationENVIRONMENTAL TOXICOLOGY
ISSN1520-4081
Volume31Issue:10Pages:1206-1216
AbstractMicrocystins (MCs) are common toxins produced by freshwater cyanobacteria, and they represent a potential health risk to aquatic organisms and animals, including humans. Specific inhibition of protein phosphatases 1 and 2A is considered the typical mechanism of MCs toxicity, but the exact mechanism has not been fully elucidated. To further our understanding of the toxicological mechanisms induced by MCs, this study is the first to use a chemical proteomic approach to screen proteins that exhibit special interactions with MC-arginine-arginine (MC-RR) from zebrafish (Danio rerio) liver. Seventeen proteins were identified via affinity blocking test. Integration of the results of previous studies and this study revealed that these proteins play a crucial role in various toxic phenomena of liver induced by MCs, such as the disruption of cytoskeleton assembly, oxidative stress, and metabolic disorder. Moreover, in addition to inhibition of protein phosphate activity, the overall toxicity of MCs was simultaneously modulated by the distribution of MCs in cells and their interactions with other target proteins. These results provide new insight into the mechanisms of hepatotoxicity induced by MCs. (c) 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1206-1216, 2016.
SubtypeArticle
KeywordMicrocystin-rr Chemical Proteomics Toxicological Mechanisms Zebrafish Liver
DOI10.1002/tox.22128
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
Indexed BySCI
Funding OrganizationNational Natural Science Foundation of China(31322013 ; National Natural Science Foundation of China(31322013 ; 31070457) ; 31070457) ; National Natural Science Foundation of China(31322013 ; National Natural Science Foundation of China(31322013 ; 31070457) ; 31070457)
Language英语
WOS Research AreaEnvironmental Sciences & Ecology ; Toxicology ; Water Resources
WOS SubjectEnvironmental Sciences ; Toxicology ; Water Resources
WOS KeywordTANDEM MASS-SPECTROMETRY ; AFFINITY-CHROMATOGRAPHY ; RAT HEPATOCYTES ; INTERMEDIATE-FILAMENTS ; PROTEIN PHOSPHATASES ; PROTEOLYTIC-ENZYMES ; CELLULAR-RESPONSES ; SERINE PROTEASES ; MOUSE-LIVER ; FISH LIVER
WOS IDWOS:000383611900005
Funding OrganizationNational Natural Science Foundation of China(31322013 ; National Natural Science Foundation of China(31322013 ; 31070457) ; 31070457) ; National Natural Science Foundation of China(31322013 ; National Natural Science Foundation of China(31322013 ; 31070457) ; 31070457)
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/28567
Collection淡水生态学研究中心
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Donghu Expt Stn Lake Ecosyst, Donghu South Rd 7, Wuhan 430072, Peoples R China
2.Nanchang Univ, Basic Chem Expt Ctr, Nanchang 330031, Jiangxi, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Tuo, Xun,Chen, Jun,Zhao, Sujuan,et al. Chemical proteomic analysis of the potential toxicological mechanisms of microcystin-RR in zebrafish (Danio rerio) liver[J]. ENVIRONMENTAL TOXICOLOGY,2016,31(10):1206-1216.
APA Tuo, Xun,Chen, Jun,Zhao, Sujuan,&Xie, Ping.(2016).Chemical proteomic analysis of the potential toxicological mechanisms of microcystin-RR in zebrafish (Danio rerio) liver.ENVIRONMENTAL TOXICOLOGY,31(10),1206-1216.
MLA Tuo, Xun,et al."Chemical proteomic analysis of the potential toxicological mechanisms of microcystin-RR in zebrafish (Danio rerio) liver".ENVIRONMENTAL TOXICOLOGY 31.10(2016):1206-1216.
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