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Transgenic expression of delta-6 and delta-15 fatty acid desaturases enhances omega-3 polyunsaturated fatty acid accumulation in Synechocystissp. PCC6803
Chen,Gao1,2; Qu,Shujie5; Wang,Qiang4; Bian,Fei1,2; Peng,Zhenying1,2; Zhang,Yan1,2; Ge,Haitao6; Yu,Jinhui1,2; Xuan,Ning1,2; Bi,Yuping1,2; He,Qingfang1,2,3
Corresponding AuthorBi,Yuping(yuping_bi@hotmail.com) ; He,Qingfang(qfhe@ualr.edu)
2014-03-01
Source PublicationBiotechnology for Biofuels
ISSN1754-6834
Volume7Issue:1
AbstractAbstractBackgroundPolyunsaturated fatty acids (PUFAs), which contain two or more double bonds in their backbone, are the focus of intensive global research, because of their nutritional value, medicinal applications, and potential use as biofuel. However, the ability to produce these economically important compounds is limited, because it is both expensive and technically challenging to separate omega-3 polyunsaturated fatty acids (ω-3 PUFAs) from natural oils. Although the biosynthetic pathways of some plant and microalgal ω-3 PUFAs have been deciphered, current understanding of the correlation between fatty acid desaturase content and fatty acid synthesis in Synechocystis sp. PCC6803 is incomplete.ResultsWe constructed a series of homologous vectors for the endogenous and exogenous expression of Δ6 and Δ15 fatty acid desaturases under the control of the photosynthesis psbA2 promoter in transgenic Synechocystis sp. PCC6803. We generated six homologous recombinants, harboring various fatty acid desaturase genes from Synechocystis sp. PCC6803, Gibberella fujikuroi and Mortierella alpina. These lines produced up to 8.9?mg/l of α-linolenic acid (ALA) and 4.1?mg/l of stearidonic acid (SDA), which are more than six times the corresponding wild-type levels, at 20°C and 30°C. Thus, transgenic expression of Δ6 and Δ15 fatty acid desaturases enhances the accumulation of specific ω-3 PUFAs in Synechocystis sp. PCC6803.ConclusionsIn the blue-green alga Synechocystis sp. PCC6803, overexpression of endogenous and exogenous genes encoding PUFA desaturases markedly increased accumulation of ALA and SDA and decreased accumulation of linoleic acid and γ-linolenic acid. This study lays the foundation for increasing the fatty acid content of cyanobacteria and, ultimately, for producing nutritional and medicinal products with high levels of essential ω-3 PUFAs.
KeywordOmega-3 polyunsaturated fatty acids Gene expression Fatty acid desaturase Synechocystis sp. PCC6803
DOI10.1186/1754-6834-7-32
Language英语
WOS IDBMC:10.1186/1754-6834-7-32
PublisherBioMed Central
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/28556
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorBi,Yuping; He,Qingfang
Affiliation1.Shandong Academy of Agricultural Sciences; Biotechnology Research Center
2.Ecology and Physiology of Crops; Shandong Provincial Key Laboratory of Genetic Improvement
3.University of Arkansas; Department of Applied Science
4.Institute of Hydrobiology, Chinese Academy of Sciences; Key Laboratory of Algal Biology
5.Test Base Service Center, Shandong Academy of Agricultural Sciences
6.Shandong University; School of Life Science
Recommended Citation
GB/T 7714
Chen,Gao,Qu,Shujie,Wang,Qiang,et al. Transgenic expression of delta-6 and delta-15 fatty acid desaturases enhances omega-3 polyunsaturated fatty acid accumulation in Synechocystissp. PCC6803[J]. Biotechnology for Biofuels,2014,7(1).
APA Chen,Gao.,Qu,Shujie.,Wang,Qiang.,Bian,Fei.,Peng,Zhenying.,...&He,Qingfang.(2014).Transgenic expression of delta-6 and delta-15 fatty acid desaturases enhances omega-3 polyunsaturated fatty acid accumulation in Synechocystissp. PCC6803.Biotechnology for Biofuels,7(1).
MLA Chen,Gao,et al."Transgenic expression of delta-6 and delta-15 fatty acid desaturases enhances omega-3 polyunsaturated fatty acid accumulation in Synechocystissp. PCC6803".Biotechnology for Biofuels 7.1(2014).
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