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An extracytoplasmic function sigma factor-dependent periplasmic glutathione peroxidase is involved in oxidative stress response of Shewanella oneidensis
Dai,Jingcheng1,2; Wei,Hehong1,2; Tian,Chunyuan3; Damron,Fredrick Heath4; Zhou,Jizhong5; Qiu,Dongru1,2
Corresponding AuthorZhou,Jizhong(jzhou@ou.edu) ; Qiu,Dongru(qiu@ihb.ac.cn)
2015-02-18
Source PublicationBMC Microbiology
ISSN1471-2180
Volume15Issue:1
AbstractAbstractBackgroundBacteria use alternative sigma factors (σs) to regulate condition-specific gene expression for survival and Shewanella harbors multiple ECF (extracytoplasmic function) σ genes and cognate anti-sigma factor genes. Here we comparatively analyzed two of the rpoE-like operons in the strain MR-1: rpoE-rseA-rseB-rseC and rpoE2-chrR.ResultsRpoE was important for bacterial growth at low and high temperatures, in the minimal medium, and high salinity. The degP/htrA orthologue, required for growth of Escherichia coli and Pseudomonas aeruginosa at high temperature, is absent in Shewanella, while the degQ gene is RpoE-regulated and is required for bacterial growth at high temperature. RpoE2 was essential for the optimal growth in oxidative stress conditions because the rpoE2 mutant was sensitive to hydrogen peroxide and paraquat. The operon encoding a ferrochelatase paralogue (HemH2) and a periplasmic glutathione peroxidase (PgpD) was identified as RpoE2-dependent. PgpD exhibited higher activities and played a more important role in the oxidative stress responses than the cytoplasmic glutathione peroxidase CgpD under tested conditions. The rpoE2-chrR operon and the identified regulon genes, including pgpD and hemH2, are coincidently absent in several psychrophilic and/or deep-sea Shewanella strains.ConclusionIn S. oneidensis MR-1, the RpoE-dependent degQ gene is required for optimal growth under high temperature. The rpoE2 and RpoE2-dependent pgpD gene encoding a periplasmic glutathione peroxidase are involved in oxidative stress responses. But rpoE2 is not required for bacterial growth at low temperature and it even affected bacterial growth under salt stress, indicating that there is a tradeoff between the salt resistance and RpoE2-mediated oxidative stress responses.
KeywordPeriplasmic glutathione peroxidase Shewanella ECF sigma factor Oxidative stress response
DOI10.1186/s12866-015-0357-0
Language英语
WOS IDBMC:10.1186/s12866-015-0357-0
PublisherBioMed Central
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/28539
Corresponding AuthorZhou,Jizhong; Qiu,Dongru
Affiliation1.Institute of Hydrobiology, Chinese Academy of Sciences
2.University of Chinese Academy of Sciences
3.Hubei University of Engineering; School of Life Sciences and Technology
4.University of Virginia; Division of Infectious Diseases and International Health, Department of Medicine
5.The University of Oklahoma, Stephenson Research and Technology Center; Institute for Environmental Genomics and Department of Botany and Microbiology
Recommended Citation
GB/T 7714
Dai,Jingcheng,Wei,Hehong,Tian,Chunyuan,et al. An extracytoplasmic function sigma factor-dependent periplasmic glutathione peroxidase is involved in oxidative stress response of Shewanella oneidensis[J]. BMC Microbiology,2015,15(1).
APA Dai,Jingcheng,Wei,Hehong,Tian,Chunyuan,Damron,Fredrick Heath,Zhou,Jizhong,&Qiu,Dongru.(2015).An extracytoplasmic function sigma factor-dependent periplasmic glutathione peroxidase is involved in oxidative stress response of Shewanella oneidensis.BMC Microbiology,15(1).
MLA Dai,Jingcheng,et al."An extracytoplasmic function sigma factor-dependent periplasmic glutathione peroxidase is involved in oxidative stress response of Shewanella oneidensis".BMC Microbiology 15.1(2015).
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