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Programmed cell death in the cyanobacterium Microcystis aeruginosa induced by allelopathic effect of submerged macrophyte Myriophyllum spicatum in co-culture system
He, Yan1,2; Zhou, Qiao-Hong1; Liu, Bi-Yun1; Cheng, Long1; Tian, Yun1; Zhang, Yong-Yuan1; Wu, Zhen-Bin1
2016-10-01
Source PublicationJOURNAL OF APPLIED PHYCOLOGY
ISSN0921-8971
Volume28Issue:5Pages:2805-2814
AbstractThis investigation demonstrates that programmed cell death (PCD) in a cyanobacterium, Microcystis aeruginosa, resulting from allelopathic stress induced by a submerged macrophyte, Myriophyllum spicatum, in a co-culture system. The hallmarks of PCD, caspase-3-like protease activity, DNA fragmentation, and destruction of cell ultrastructure, as well as intracellular PCD signaling radicals, reactive oxygen species (ROS), and nitric oxide (NO), were measured in M. aeruginosa cells co-cultured with M. spicatum for 7 days. The results showed a dose-response relationship between M. spicatum biomass and M. aeruginosa mortality. A caspase-3-like protease was activated and elevated from day 3. Thylakoid disintegration, cytoplasmic vacuolation, and fuzzy nuclear zone were observed by transmission electron microscopy, and distinct DNA fragmentation was detected in M. aeruginosa cells at a M. spicatum biomass of 6.0 g fresh weight (FW) L-1 during the 7 days. Allelochemicals of total phenolic compounds (TPCs) were determined in co-culture water, and the concentration increased with increasing of M. spicatum biomass and co-culture time. Compared with the level of ROS production in the control group, a significant overproduction of ROS was detected in M. aeruginosa cells in the treatment group, and this was positively correlated with TPC concentration. Furthermore, the level of intracellular NO increased with the percent mortality of M. aeruginosa. The results indicated that a PCD pathway was induced in the cyanobacterium M. aeruginosa when co-cultured with the submerged macrophyte M. spicatum.
SubtypeArticle
KeywordMicrocystis Aeruginosa Myriophyllum Spicatum Allelopathic Effect Ros/no Caspase-3-like Activity Programmed Cell Death
DOI10.1007/s10811-016-0814-7
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNational Nature Science Foundation of China(51178452 ; National Nature Science Foundation of China(51178452 ; Major Science and Technology Program for Water Pollution Control and Treatment of China(2012ZX07101007-005) ; Major Science and Technology Program for Water Pollution Control and Treatment of China(2012ZX07101007-005) ; State Key Laboratory of Freshwater Ecology and Biotechnology Program(2013FB20) ; State Key Laboratory of Freshwater Ecology and Biotechnology Program(2013FB20) ; 50909091 ; 50909091 ; 31123001) ; 31123001) ; National Nature Science Foundation of China(51178452 ; National Nature Science Foundation of China(51178452 ; Major Science and Technology Program for Water Pollution Control and Treatment of China(2012ZX07101007-005) ; Major Science and Technology Program for Water Pollution Control and Treatment of China(2012ZX07101007-005) ; State Key Laboratory of Freshwater Ecology and Biotechnology Program(2013FB20) ; State Key Laboratory of Freshwater Ecology and Biotechnology Program(2013FB20) ; 50909091 ; 50909091 ; 31123001) ; 31123001)
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS SubjectBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS KeywordGREEN-ALGA MICRASTERIAS ; NITRIC-OXIDE ; GROWTH-INHIBITION ; (-)-EPIGALLOCATECHIN GALLATE ; CHLAMYDOMONAS-REINHARDTII ; HYDROGEN-PEROXIDE ; SHALLOW LAKES ; STRESS ; APOPTOSIS ; MECHANISMS
WOS IDWOS:000383569600018
Funding OrganizationNational Nature Science Foundation of China(51178452 ; National Nature Science Foundation of China(51178452 ; Major Science and Technology Program for Water Pollution Control and Treatment of China(2012ZX07101007-005) ; Major Science and Technology Program for Water Pollution Control and Treatment of China(2012ZX07101007-005) ; State Key Laboratory of Freshwater Ecology and Biotechnology Program(2013FB20) ; State Key Laboratory of Freshwater Ecology and Biotechnology Program(2013FB20) ; 50909091 ; 50909091 ; 31123001) ; 31123001) ; National Nature Science Foundation of China(51178452 ; National Nature Science Foundation of China(51178452 ; Major Science and Technology Program for Water Pollution Control and Treatment of China(2012ZX07101007-005) ; Major Science and Technology Program for Water Pollution Control and Treatment of China(2012ZX07101007-005) ; State Key Laboratory of Freshwater Ecology and Biotechnology Program(2013FB20) ; State Key Laboratory of Freshwater Ecology and Biotechnology Program(2013FB20) ; 50909091 ; 50909091 ; 31123001) ; 31123001)
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/28393
Collection水环境工程研究中心
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
He, Yan,Zhou, Qiao-Hong,Liu, Bi-Yun,et al. Programmed cell death in the cyanobacterium Microcystis aeruginosa induced by allelopathic effect of submerged macrophyte Myriophyllum spicatum in co-culture system[J]. JOURNAL OF APPLIED PHYCOLOGY,2016,28(5):2805-2814.
APA He, Yan.,Zhou, Qiao-Hong.,Liu, Bi-Yun.,Cheng, Long.,Tian, Yun.,...&Wu, Zhen-Bin.(2016).Programmed cell death in the cyanobacterium Microcystis aeruginosa induced by allelopathic effect of submerged macrophyte Myriophyllum spicatum in co-culture system.JOURNAL OF APPLIED PHYCOLOGY,28(5),2805-2814.
MLA He, Yan,et al."Programmed cell death in the cyanobacterium Microcystis aeruginosa induced by allelopathic effect of submerged macrophyte Myriophyllum spicatum in co-culture system".JOURNAL OF APPLIED PHYCOLOGY 28.5(2016):2805-2814.
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