IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心
Disruption of dmc1 Produces Abnormal Sperm in Medaka (Oryzias latipes)
Chen, Ji1; Cui, Xiaojuan1; Jia, Shaoting1,2; Luo, Daji3; Cao, Mengxi1; Zhang, Yunsheng1; Hu, Hongling1,2; Huang, Kaiyao1; Zhu, Zuoyan1; Hu, Wei1
2016-08-02
Source PublicationScientific Reports
ISSN2045-2322
Volume6
AbstractDMC1 is a recombinase that is essential for meiotic synapsis. Experiments in extensive species of eukaryotes have indicated the independent role of DMC1 in repairing double strand breaks (DSBs) produced during meiosis I. Mutation of dmc1 in mice and human often leads to obstacles in spermatogenesis and male sterility. Here, we report on the disruption of dmc1 in male medaka (Oryzias latipes). Synapsis was disturbed in the mutant medaka testis nuclei, as observed in mice and other organisms. Unexpectedly, the mutant medaka could produce a few sperm and, although most of these had multiple tail or multiple head malformations, some of them could swim, and few of them even had insemination ability. Our transcriptome analysis showed that there was not a remarkable change in the expression of most of the genes involved in the pathways associated with the meiotic DNA repair and flagella assembly. Our results provided an indication of the accessory mechanisms that might be involved in the repair of DSBs during meiosis. In a species besides humans, we provided evidence that disorders in meiosis recombination might lead to the malformation of sperm.
SubtypeArticle
DOI10.1038/srep30912
WOS HeadingsScience & Technology
Indexed BySCI
Funding OrganizationNational Natural Science Foundation of China(31325026 ; National Natural Science Foundation of China(31325026 ; Key Research Program of the Chinese Academy of Sciences(XDA08010106) ; Key Research Program of the Chinese Academy of Sciences(XDA08010106) ; Natural Science Foundation of Hubei Province(2015CFA007) ; Natural Science Foundation of Hubei Province(2015CFA007) ; 31461163006) ; 31461163006) ; National Natural Science Foundation of China(31325026 ; National Natural Science Foundation of China(31325026 ; Key Research Program of the Chinese Academy of Sciences(XDA08010106) ; Key Research Program of the Chinese Academy of Sciences(XDA08010106) ; Natural Science Foundation of Hubei Province(2015CFA007) ; Natural Science Foundation of Hubei Province(2015CFA007) ; 31461163006) ; 31461163006)
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS KeywordDOUBLE-STRAND BREAKS ; MALE-INFERTILITY ; SYNAPTONEMAL COMPLEX ; HUMAN ANEUPLOIDY ; MEIOSIS ; GENE ; ZEBRAFISH ; FISH ; SPERMATOGENESIS ; RECOMBINATION
WOS IDWOS:000380639200002
Funding OrganizationNational Natural Science Foundation of China(31325026 ; National Natural Science Foundation of China(31325026 ; Key Research Program of the Chinese Academy of Sciences(XDA08010106) ; Key Research Program of the Chinese Academy of Sciences(XDA08010106) ; Natural Science Foundation of Hubei Province(2015CFA007) ; Natural Science Foundation of Hubei Province(2015CFA007) ; 31461163006) ; 31461163006) ; National Natural Science Foundation of China(31325026 ; National Natural Science Foundation of China(31325026 ; Key Research Program of the Chinese Academy of Sciences(XDA08010106) ; Key Research Program of the Chinese Academy of Sciences(XDA08010106) ; Natural Science Foundation of Hubei Province(2015CFA007) ; Natural Science Foundation of Hubei Province(2015CFA007) ; 31461163006) ; 31461163006)
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/28389
Collection鱼类生物学及渔业生物技术研究中心
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, 7 Donghu South Rd, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Wuhan Univ, Sch Basic Med Sci, Dept Genet, 185 Donghu East Rd, Wuhan 430071, Peoples R China
Recommended Citation
GB/T 7714
Chen, Ji,Cui, Xiaojuan,Jia, Shaoting,et al. Disruption of dmc1 Produces Abnormal Sperm in Medaka (Oryzias latipes)[J]. Scientific Reports,2016,6.
APA Chen, Ji.,Cui, Xiaojuan.,Jia, Shaoting.,Luo, Daji.,Cao, Mengxi.,...&Hu, Wei.(2016).Disruption of dmc1 Produces Abnormal Sperm in Medaka (Oryzias latipes).Scientific Reports,6.
MLA Chen, Ji,et al."Disruption of dmc1 Produces Abnormal Sperm in Medaka (Oryzias latipes)".Scientific Reports 6(2016).
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