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Differential Regulation of the Two Ferrochelatase Paralogues in Shewanella loihica PV-4 in Response to Environmental Stresses
Qiu, Dongru1,2,3; Xie, Ming3; Dai, Jingcheng1,2; An, Weixing1,2; Wei, Hehong1,2; Tian, Chunyuan4; Kempher, Megan L.3; Zhou, Aifen3; He, Zhili3; Gu, Baohua5; Zhou, Jizhong3,6,7
2016-09-01
Source PublicationAPPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN0099-2240
Volume82Issue:17Pages:5077-5088
AbstractDetermining the function and regulation of paralogues is important in understanding microbial functional genomics and environmental adaptation. Heme homeostasis is crucial for the survival of environmental microorganisms. Most Shewanella species encode two paralogues of ferrochelatase, the terminal enzyme in the heme biosynthesis pathway. The function and transcriptional regulation of two ferrochelatase genes, hemH1 and hemH2, were investigated in Shewanella loihica PV-4. The disruption of hemH1 but not hemH2 resulted in a significant accumulation of extracellular protoporphyrin IX (PPIX), the precursor to heme, and decreased intracellular heme levels. hemH1 was constitutively expressed, and the expression of hemH2 increased when hemH1 was disrupted. The transcription of hemH1 was regulated by the housekeeping sigma factor RpoD and potentially regulated by OxyR, while hemH2 appeared to be regulated by the oxidative stress-associated sigma factor RpoE2. When an oxidative stress condition was mimicked by adding H2O2 to the medium or exposing the culture to light, PPIX accumulation was suppressed in the Delta hemH1 mutant. Consistently, transcriptome analysis indicated enhanced iron uptake and suppressed heme synthesis in the Delta hemH1 mutant. These data indicate that the two paralogues are functional in the heme synthesis pathway but regulated by environmental conditions, providing insights into the understanding of bacterial response to environmental stresses and a great potential to commercially produce porphyrin compounds.
SubtypeArticle
DOI10.1128/AEM.00203-16
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationDOE(DE-FG02-07ER64383) ; DOE(DE-FG02-07ER64383) ; Chinese Academy of Sciences(Y15103-1-401) ; Chinese Academy of Sciences(Y15103-1-401) ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; (WO2014144329 A2) ; (WO2014144329 A2) ; DOE(DE-FG02-07ER64383) ; DOE(DE-FG02-07ER64383) ; Chinese Academy of Sciences(Y15103-1-401) ; Chinese Academy of Sciences(Y15103-1-401) ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; (WO2014144329 A2) ; (WO2014144329 A2)
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Microbiology
WOS SubjectBiotechnology & Applied Microbiology ; Microbiology
WOS KeywordHEME-BIOSYNTHESIS PATHWAY ; LIGHT-SENSITIVE MUTANTS ; PROTOPORPHYRIN-IX ; ESCHERICHIA-COLI ; ONEIDENSIS MR-1 ; SYSTEMS BIOLOGY ; SIGMA-FACTORS ; GENES ; GENUS ; IDENTIFICATION
WOS IDWOS:000381500700003
Funding OrganizationDOE(DE-FG02-07ER64383) ; DOE(DE-FG02-07ER64383) ; Chinese Academy of Sciences(Y15103-1-401) ; Chinese Academy of Sciences(Y15103-1-401) ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; (WO2014144329 A2) ; (WO2014144329 A2) ; DOE(DE-FG02-07ER64383) ; DOE(DE-FG02-07ER64383) ; Chinese Academy of Sciences(Y15103-1-401) ; Chinese Academy of Sciences(Y15103-1-401) ; One-Hundred Scholar Award ; One-Hundred Scholar Award ; (WO2014144329 A2) ; (WO2014144329 A2)
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/28384
Collection水环境工程研究中心
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Univ Oklahoma, Dept Microbiol & Plant Biol, Inst Environm Gen, Norman, OK 73019 USA
4.Hubei Engn Univ, Sch Life Sci & Technol, Xiaogan, Peoples R China
5.Oak Ridge Natl Lab, Div Earth Sci, Oak Ridge, TN 37831 USA
6.Lawrence Berkeley Natl Lab, Div Earth Sci, Berkeley, CA 94720 USA
7.Tsinghua Univ, Sch Environm, State Key Joint Lab Environm Simulat & Pollut Con, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Qiu, Dongru,Xie, Ming,Dai, Jingcheng,et al. Differential Regulation of the Two Ferrochelatase Paralogues in Shewanella loihica PV-4 in Response to Environmental Stresses[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2016,82(17):5077-5088.
APA Qiu, Dongru.,Xie, Ming.,Dai, Jingcheng.,An, Weixing.,Wei, Hehong.,...&Zhou, Jizhong.(2016).Differential Regulation of the Two Ferrochelatase Paralogues in Shewanella loihica PV-4 in Response to Environmental Stresses.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,82(17),5077-5088.
MLA Qiu, Dongru,et al."Differential Regulation of the Two Ferrochelatase Paralogues in Shewanella loihica PV-4 in Response to Environmental Stresses".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 82.17(2016):5077-5088.
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