Epidermal growth factor-induced stimulation of proliferation and gene expression changes in the hypotrichous ciliate, Stylonychia lemnae
Mu, Weijie1; Wang, Qi1,2; Bourland, William A.3; Jiang, Chuanqi2; Yuan, Dongxia2; Pan, Xuming1; Miao, Wei2; Chen, Ying1; Xiong, Jie2
2016-10-30
Source PublicationGENE
ISSN0378-1119
Volume592Issue:1Pages:186-192
AbstractEpidermal growth factor (EGF) induces proliferation of epidermal and epithelial tissues in mammals. However, the effect of EGF on the single-celled eukaryotes is not well characterized, especially in the protists. Ciliates, an important group of protists, are well characterized as both pollution indicators and model organisms for research. Stylonychia lemnae, is one of the most common free-living ciliates, widely distributed in ponds, rivers and marshes. Here, we report the role of EGF on cell proliferation stimulation in S. lemnae. The growth curve of S. lemnae was established, and the stimulation effect of EGF on the proliferation of S. lemnae was investigated. Based on the results, potential EGF receptors were identified in S. lemnae according to the conserved domains and gene expression. Differential gene expression revealed that EGF-induced genes in other organisms (e.g. antioxidant) also up-regulated in S. lemnae cells at propagation stages. In addition, our results showed that EGF could up-regulate the signal transduction-related processes in the decline stage of S. lemnae cells, indicating its potential function in apoptosis inhibition. In summary, this study reports findings of the first investigation of EGF effects in hypotrich ciliates, and establishes an additional system for the study of the molecular mechanisms of EGF actions in eukaryotic cell division and proliferation. (C) 2016 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordEpidermal Growth Factor Proliferation Stimulation Stylonychia Lemnae Epidermal Growth Factor Receptor Gene Expression
DOI10.1016/j.gene.2016.08.004
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNational Natural Science Foundation of China(31101613 ; National Natural Science Foundation of China(31101613 ; 31471950 ; 31471950 ; 31501844) ; 31501844) ; National Natural Science Foundation of China(31101613 ; National Natural Science Foundation of China(31101613 ; 31471950 ; 31471950 ; 31501844) ; 31501844)
Language英语
WOS Research AreaGenetics & Heredity
WOS SubjectGenetics & Heredity
WOS KeywordFACTOR RECEPTOR ; ACTIVATION ; ONTOLOGY ; SYSTEM ; ENZYME ; TOOL ; EGF
WOS IDWOS:000383304600024
Funding OrganizationNational Natural Science Foundation of China(31101613 ; National Natural Science Foundation of China(31101613 ; 31471950 ; 31471950 ; 31501844) ; 31501844) ; National Natural Science Foundation of China(31101613 ; National Natural Science Foundation of China(31101613 ; 31471950 ; 31471950 ; 31501844) ; 31501844)
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/28374
Collection藻类生物学及应用研究中心_水生生物分子与细胞生物学研究中心
Affiliation1.Harbin Normal Univ, Coll Life Sci & Technol, Harbin 150025, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China
3.Boise State Univ, Dept Biol Sci, Boise, ID 83725 USA
Recommended Citation
GB/T 7714
Mu, Weijie,Wang, Qi,Bourland, William A.,et al. Epidermal growth factor-induced stimulation of proliferation and gene expression changes in the hypotrichous ciliate, Stylonychia lemnae[J]. GENE,2016,592(1):186-192.
APA Mu, Weijie.,Wang, Qi.,Bourland, William A..,Jiang, Chuanqi.,Yuan, Dongxia.,...&Xiong, Jie.(2016).Epidermal growth factor-induced stimulation of proliferation and gene expression changes in the hypotrichous ciliate, Stylonychia lemnae.GENE,592(1),186-192.
MLA Mu, Weijie,et al."Epidermal growth factor-induced stimulation of proliferation and gene expression changes in the hypotrichous ciliate, Stylonychia lemnae".GENE 592.1(2016):186-192.
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