Cdk3, a conjugation-specific cyclin-dependent kinase, is essential for the initiation of meiosis in Tetrahymena thermophila
Yan, Guan-xiong1,2; Zhang, Jing1,2; Shodhan, Anura3; Tian, Miao1,3,4; Miao, Wei1
2016
Source PublicationCELL CYCLE
ISSN1538-4101
Volume15Issue:18Pages:2506-2514
AbstractMeiosis is an important process in sexual reproduction. Meiosis initiation has been found to be highly diverse among species. In yeast, it has been established that cyclin-dependent kinases (Cdks) and cyclins are essential components in the meiosis initiation pathway. In this study, we identified 4 Cdks in the model ciliate, Tetrahymena thermophila, and we found one of them, Cdk3, which is specifically expressed during early conjugation, to be essential for meiosis initiation. Cdk3 deletion led to arrest at the pair formation stage of conjugation. We then confirmed that Cdk3 acts upstream of double-strand break (DSB) formation. Moreover, we detected that Cdk3 is necessary for the expression of many genes involved in early meiotic events. Through proteomic quantification of phosphorylation, co-expression analysis and RNA-Seq analyses, we identified a conjugation-specific cyclin, Cyc2, which most likely partners with Cdk3 to initiate meiosis.
SubtypeArticle
KeywordConjugation Cyclin-dependent Kinase Meiosis Initiation Sexual Reproduction Tetrahymena Thermophila
DOI10.1080/15384101.2016.1207838
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationInternational Science & Technology Cooperation Program of China(2013DFG32390) ; International Science & Technology Cooperation Program of China(2013DFG32390) ; OEAD (Austria)(CN10/2013) ; OEAD (Austria)(CN10/2013) ; Austrian Science Fund (FWF)(P27313-B20 ; Austrian Science Fund (FWF)(P27313-B20 ; W1238-B20) ; W1238-B20) ; International Science & Technology Cooperation Program of China(2013DFG32390) ; International Science & Technology Cooperation Program of China(2013DFG32390) ; OEAD (Austria)(CN10/2013) ; OEAD (Austria)(CN10/2013) ; Austrian Science Fund (FWF)(P27313-B20 ; Austrian Science Fund (FWF)(P27313-B20 ; W1238-B20) ; W1238-B20)
Language英语
WOS Research AreaCell Biology
WOS SubjectCell Biology
WOS KeywordCELL-CYCLE ; DNA-REPLICATION ; PROTEIN-KINASE ; GENE ONTOLOGY ; FISSION YEAST ; RNA-SEQ ; PHOSPHORYLATION ; IME2 ; ACTIVATION ; GERMLINE
WOS IDWOS:000384022200021
Funding OrganizationInternational Science & Technology Cooperation Program of China(2013DFG32390) ; International Science & Technology Cooperation Program of China(2013DFG32390) ; OEAD (Austria)(CN10/2013) ; OEAD (Austria)(CN10/2013) ; Austrian Science Fund (FWF)(P27313-B20 ; Austrian Science Fund (FWF)(P27313-B20 ; W1238-B20) ; W1238-B20) ; International Science & Technology Cooperation Program of China(2013DFG32390) ; International Science & Technology Cooperation Program of China(2013DFG32390) ; OEAD (Austria)(CN10/2013) ; OEAD (Austria)(CN10/2013) ; Austrian Science Fund (FWF)(P27313-B20 ; Austrian Science Fund (FWF)(P27313-B20 ; W1238-B20) ; W1238-B20)
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/28357
Collection藻类生物学及应用研究中心_水生生物分子与细胞生物学研究中心
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Univ Vienna, Dept Chromosome Biol, Ctr Mol Biol, Vienna, Austria
4.Univ Vienna, Max F Perutz Labs, Ctr Mol Biol, Vienna, Austria
Recommended Citation
GB/T 7714
Yan, Guan-xiong,Zhang, Jing,Shodhan, Anura,et al. Cdk3, a conjugation-specific cyclin-dependent kinase, is essential for the initiation of meiosis in Tetrahymena thermophila[J]. CELL CYCLE,2016,15(18):2506-2514.
APA Yan, Guan-xiong,Zhang, Jing,Shodhan, Anura,Tian, Miao,&Miao, Wei.(2016).Cdk3, a conjugation-specific cyclin-dependent kinase, is essential for the initiation of meiosis in Tetrahymena thermophila.CELL CYCLE,15(18),2506-2514.
MLA Yan, Guan-xiong,et al."Cdk3, a conjugation-specific cyclin-dependent kinase, is essential for the initiation of meiosis in Tetrahymena thermophila".CELL CYCLE 15.18(2016):2506-2514.
Files in This Item:
File Name/Size DocType Version Access License
222.pdf(2126KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Yan, Guan-xiong]'s Articles
[Zhang, Jing]'s Articles
[Shodhan, Anura]'s Articles
Baidu academic
Similar articles in Baidu academic
[Yan, Guan-xiong]'s Articles
[Zhang, Jing]'s Articles
[Shodhan, Anura]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Yan, Guan-xiong]'s Articles
[Zhang, Jing]'s Articles
[Shodhan, Anura]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 222.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.