IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心
Molecular characterization and expression pattern of a germ cell marker gene dnd in gibel carp (Carassius gibelio)
Li, Shi-Zhu1; Liu, Wei2; Li, Zhi1; Wang, Yang1; Zhou, Li1; Yi, Mei-Sheng2; Gui, Jian-Fang1
2016-10-10
Source PublicationGENE
ISSN0378-1119
Volume591Issue:1Pages:183-190
AbstractAs a germ cell marker gene, Dead end (dnd) has been identified and characterized in many vertebrates. Recently, we created a complete germ cell-depleted gonad model by the dnd-specific morpholino-mediated knockdown approach, and revealed sex-biased gene expression alteration through utilizing unisexual gynogenetic superiority in polyploid gibel carp. However, dud and its expression pattern are still unclear in the gibel carp. In this study, we further analyzed molecular characterization of gibel carp dnd and its dynamic expression pattern during gametogenesis and embryogenesis. Similar to other homologs in vertebrates, gibel carp dnd contains a conserved RRM motif and five other motifs, and is highly evolutionary conserved in genomic organization and neighborhood gene synteny. RT-PCR and Western blot analyses showed its gonad-specific expression intensively in testis and ovary. Section in situ hybridization (SISH) and immunofluorescence localization revealed its dynamic expression pattern specific to oogenic cells and spermatogenetic cells during oogenesis and spermatogenesis. Moreover, its temporal and spatial distribution specific to PGCs were also demonstrated by RT-PCR and whole mount in situ hybridization (WISH) during embryogenesis. Therefore, gibel carp Dnd is a conserved germ cell marker during gametogenesis, and its maternal transcript is also a useful marker for tracing PGC specification and migration. (C) 2016 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordDead End Primordial Germ Cell Germ Cells Oogenesis Spermatogenesis
DOI10.1016/j.gene.2016.07.027
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationStrategic Priority Research Program of the Chinese Academy of Sciences(XDA08030201) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA08030201) ; earmarked fund for Modern Agro-industry Technology Research System(NYCYTX-49) ; earmarked fund for Modern Agro-industry Technology Research System(NYCYTX-49) ; National Natural Science Foundation(31123001) ; National Natural Science Foundation(31123001) ; National Science and Technology Support Program(2012BA026B02) ; National Science and Technology Support Program(2012BA026B02) ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2011FBZ22) ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2011FBZ22) ; Institute of Hydrobiology, CAS(Y25A17 ; Institute of Hydrobiology, CAS(Y25A17 ; Y45A171301) ; Y45A171301) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA08030201) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA08030201) ; earmarked fund for Modern Agro-industry Technology Research System(NYCYTX-49) ; earmarked fund for Modern Agro-industry Technology Research System(NYCYTX-49) ; National Natural Science Foundation(31123001) ; National Natural Science Foundation(31123001) ; National Science and Technology Support Program(2012BA026B02) ; National Science and Technology Support Program(2012BA026B02) ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2011FBZ22) ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2011FBZ22) ; Institute of Hydrobiology, CAS(Y25A17 ; Institute of Hydrobiology, CAS(Y25A17 ; Y45A171301) ; Y45A171301)
Language英语
WOS Research AreaGenetics & Heredity
WOS SubjectGenetics & Heredity
WOS KeywordDEAD-END GENE ; AURATUS-GIBELIO ; REPRODUCTION MODES ; CORTICAL GRANULES ; ZEBRAFISH EMBRYOS ; SEXUAL-DIMORPHISM ; BINDING-PROTEIN ; MESSENGER-RNA ; FISH ; OOCYTES
WOS IDWOS:000382409400026
Funding OrganizationStrategic Priority Research Program of the Chinese Academy of Sciences(XDA08030201) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA08030201) ; earmarked fund for Modern Agro-industry Technology Research System(NYCYTX-49) ; earmarked fund for Modern Agro-industry Technology Research System(NYCYTX-49) ; National Natural Science Foundation(31123001) ; National Natural Science Foundation(31123001) ; National Science and Technology Support Program(2012BA026B02) ; National Science and Technology Support Program(2012BA026B02) ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2011FBZ22) ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2011FBZ22) ; Institute of Hydrobiology, CAS(Y25A17 ; Institute of Hydrobiology, CAS(Y25A17 ; Y45A171301) ; Y45A171301) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA08030201) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA08030201) ; earmarked fund for Modern Agro-industry Technology Research System(NYCYTX-49) ; earmarked fund for Modern Agro-industry Technology Research System(NYCYTX-49) ; National Natural Science Foundation(31123001) ; National Natural Science Foundation(31123001) ; National Science and Technology Support Program(2012BA026B02) ; National Science and Technology Support Program(2012BA026B02) ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2011FBZ22) ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2011FBZ22) ; Institute of Hydrobiology, CAS(Y25A17 ; Institute of Hydrobiology, CAS(Y25A17 ; Y45A171301) ; Y45A171301)
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/28348
Collection鱼类生物学及渔业生物技术研究中心
Affiliation1.Chinese Acad Sci, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Sun Yat Sen Univ, Sch Marine Sci, Zhuhai Key Lab Marine Bioresources & Environm, Guangzhou, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Li, Shi-Zhu,Liu, Wei,Li, Zhi,et al. Molecular characterization and expression pattern of a germ cell marker gene dnd in gibel carp (Carassius gibelio)[J]. GENE,2016,591(1):183-190.
APA Li, Shi-Zhu.,Liu, Wei.,Li, Zhi.,Wang, Yang.,Zhou, Li.,...&Gui, Jian-Fang.(2016).Molecular characterization and expression pattern of a germ cell marker gene dnd in gibel carp (Carassius gibelio).GENE,591(1),183-190.
MLA Li, Shi-Zhu,et al."Molecular characterization and expression pattern of a germ cell marker gene dnd in gibel carp (Carassius gibelio)".GENE 591.1(2016):183-190.
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