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Mutations in the C-terminal region affect subcellular localization of crucian carp herpesvirus (CaHV) GPCR
Wang, Jun1; Gui, Lang2; Chen, Zong-Yan1; Zhang, Qi-Ya1
2016-08-01
Source PublicationVIRUS GENES
ISSN0920-8569
Volume52Issue:4Pages:484-494
AbstractG protein-coupled receptors (GPCRs) are known as seven transmembrane domain receptors and consequently can mediate diverse biological functions via regulation of their subcellular localization. Crucian carp herpesvirus (CaHV) was recently isolated from infected fish with acute gill hemorrhage. CaHV GPCR of 349 amino acids (aa) was identified based on amino acid identity. A series of variants with truncation/deletion/substitution mutation in the C-terminal (aa 315-349) were constructed and expressed in fathead minnow (FHM) cells. The roles of three key C-terminal regions in subcellular localization of CaHV GPCR were determined. Lysine-315 (K-315) directed the aggregation of the protein preferentially at the nuclear side. Predicted N-myristoylation site (GGGWTR, aa 335-340) was responsible for punctate distribution in periplasm or throughout the cytoplasm. Predicted phosphorylation site (SSR, aa 327-329) and GGGWTR together determined the punctate distribution in cytoplasm. Detection of organelles localization by specific markers showed that the protein retaining K-315 colocalized with the Golgi apparatus. These experiments provided first evidence that different mutations of CaHV GPCR C-terminals have different affects on the subcellular localization of fish herpesvirus-encoded GPCRs. The study provided valuable information and new insights into the precise interactions between herpesvirus and fish cells, and could also provide useful targets for antiviral agents in aquaculture.
SubtypeArticle
Keywordg Protein-coupled Receptor (Gpcr) Crucian Carp Herpesvirus (Cahv) C-terminal Subcellular Localization N-myristoylation Site
DOI10.1007/s11262-016-1325-y
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNational Natural Science Foundation of China(31430091 ; National Natural Science Foundation of China(31430091 ; Strategic Priority Research Program of Chinese Academy of Sciences(XDA08030202) ; Strategic Priority Research Program of Chinese Academy of Sciences(XDA08030202) ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2014FB02) ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2014FB02) ; 31302214 ; 31302214 ; 3141101038) ; 3141101038) ; National Natural Science Foundation of China(31430091 ; National Natural Science Foundation of China(31430091 ; Strategic Priority Research Program of Chinese Academy of Sciences(XDA08030202) ; Strategic Priority Research Program of Chinese Academy of Sciences(XDA08030202) ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2014FB02) ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2014FB02) ; 31302214 ; 31302214 ; 3141101038) ; 3141101038)
Language英语
WOS Research AreaGenetics & Heredity ; Virology
WOS SubjectGenetics & Heredity ; Virology
WOS KeywordPROTEIN-COUPLED RECEPTORS ; FLOUNDER PARALICHTHYS-OLIVACEUS ; AQUACULTURE ANIMALS ; VIRUS ; DISEASE ; CELLS ; IDENTIFICATION ; TRAFFICKING ; EXPRESSION ; INFECTION
WOS IDWOS:000382636200006
Funding OrganizationNational Natural Science Foundation of China(31430091 ; National Natural Science Foundation of China(31430091 ; Strategic Priority Research Program of Chinese Academy of Sciences(XDA08030202) ; Strategic Priority Research Program of Chinese Academy of Sciences(XDA08030202) ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2014FB02) ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2014FB02) ; 31302214 ; 31302214 ; 3141101038) ; 3141101038) ; National Natural Science Foundation of China(31430091 ; National Natural Science Foundation of China(31430091 ; Strategic Priority Research Program of Chinese Academy of Sciences(XDA08030202) ; Strategic Priority Research Program of Chinese Academy of Sciences(XDA08030202) ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2014FB02) ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2014FB02) ; 31302214 ; 31302214 ; 3141101038) ; 3141101038)
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Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/28346
Collection鱼类生物学及渔业生物技术研究中心
Affiliation1.Chinese Acad Sci, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Shanghai Ocean Univ, Fisheries & Life Sci, Shanghai 200120, Peoples R China
Recommended Citation
GB/T 7714
Wang, Jun,Gui, Lang,Chen, Zong-Yan,et al. Mutations in the C-terminal region affect subcellular localization of crucian carp herpesvirus (CaHV) GPCR[J]. VIRUS GENES,2016,52(4):484-494.
APA Wang, Jun,Gui, Lang,Chen, Zong-Yan,&Zhang, Qi-Ya.(2016).Mutations in the C-terminal region affect subcellular localization of crucian carp herpesvirus (CaHV) GPCR.VIRUS GENES,52(4),484-494.
MLA Wang, Jun,et al."Mutations in the C-terminal region affect subcellular localization of crucian carp herpesvirus (CaHV) GPCR".VIRUS GENES 52.4(2016):484-494.
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