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NOD2 in zebrafish functions in antibacterial and also antiviral responses via NF-kappa B, and also MDA5, RIG-I and MAVS
Zou, Peng Fei1,2; Chang, Ming Xian2; Li, Ying3; Xue, Na Na2; Li, Jun Hua2; Chen, Shan Nan2; Nie, Pin1,2
2016-08-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume55Pages:173-185
AbstractNOD2/RIPK2 signalling plays essential role in the modulation of innate and adaptive immunity in mammals. In this study, NOD2 was functionally characterized in zebrafish (Danio rerio), and its interaction with a receptor-interaction protein, RIPK2, and RLRs such as MDA5 and RIG-I, as well as the adaptor, MAVS was revealed in fish innate immunity. The expression of NOD2 and RIPK2 in ZF4 cells has been constitutive and can be induced by the infection of Edwardsiella tarda and SVCV. The NOD2 can sense MDP in PGN from Gram-negative and-positive bacteria. It is further revealed that the NOD2 and RIPK2 can activate NF-kappa B and IFN promoters, inducing significantly antiviral defense against SVCV infection. As observed in the reduced bacterial burden in RIPK2 overexpressed cells, RIPK2 also has a role in inhibiting the bacterial replication. The overexpression of NOD2 in zebrafish embryos resulted in the increase of immune gene expression, especially those encoding PRRs and cytokines involved in antiviral response such as MDA5, RIG-I, and type I IFNs, etc. Luciferase reporter assays and co-immunoprecipitation assays demonstrated that zebrafish NOD2 is associated with MDA5 and RIG-I in signalling pathway. In addition, it is further demonstrated that RIPK2 and MAVS in combination with NOD2 have an enhanced role in "NOD2-mediated NF-kappa B and type I IFN activation. It is concluded that teleost fish NOD2 can not only sense MDP for activating innate immunity as reported in mammals, but can also interact with other PRRs to form a network in antiviral innate response. (C) 2016 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordNod-like Receptor Nod2 Rlr Nf-kappab Zebrafish
DOI10.1016/j.fsi.2016.05.031
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNational Natural Science Foundation of China(31320103913 ; National Natural Science Foundation of China(31320103913 ; State Key Laboratory of Freshwater Ecology and Biotechnology(2012FB14) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2012FB14) ; 31372531 ; 31372531 ; 31402273) ; 31402273) ; National Natural Science Foundation of China(31320103913 ; National Natural Science Foundation of China(31320103913 ; State Key Laboratory of Freshwater Ecology and Biotechnology(2012FB14) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2012FB14) ; 31372531 ; 31372531 ; 31402273) ; 31402273)
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS KeywordINNATE IMMUNE-RESPONSES ; SIGNALING PATHWAYS ; PATHOGEN RECOGNITION ; HOST RECOGNITION ; CARD15 MUTATIONS ; BLAU-SYNDROME ; ACTIVATION ; GENE ; INDUCTION ; PEPTIDOGLYCAN
WOS IDWOS:000381537000017
Funding OrganizationNational Natural Science Foundation of China(31320103913 ; National Natural Science Foundation of China(31320103913 ; State Key Laboratory of Freshwater Ecology and Biotechnology(2012FB14) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2012FB14) ; 31372531 ; 31372531 ; 31402273) ; 31402273) ; National Natural Science Foundation of China(31320103913 ; National Natural Science Foundation of China(31320103913 ; State Key Laboratory of Freshwater Ecology and Biotechnology(2012FB14) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2012FB14) ; 31372531 ; 31372531 ; 31402273) ; 31402273)
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/28343
Collection鱼类生物学及渔业生物技术研究中心
Affiliation1.Jimei Univ, Coll Fisheries, 43 Yindou Rd, Xiamen 361021, Fujian Province, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
3.Xiamen Univ, Tan Kah Kee Coll, Dept Environm Sci & Engn, Zhangzhou 363105, Fujian Province, Peoples R China
Recommended Citation
GB/T 7714
Zou, Peng Fei,Chang, Ming Xian,Li, Ying,et al. NOD2 in zebrafish functions in antibacterial and also antiviral responses via NF-kappa B, and also MDA5, RIG-I and MAVS[J]. FISH & SHELLFISH IMMUNOLOGY,2016,55:173-185.
APA Zou, Peng Fei.,Chang, Ming Xian.,Li, Ying.,Xue, Na Na.,Li, Jun Hua.,...&Nie, Pin.(2016).NOD2 in zebrafish functions in antibacterial and also antiviral responses via NF-kappa B, and also MDA5, RIG-I and MAVS.FISH & SHELLFISH IMMUNOLOGY,55,173-185.
MLA Zou, Peng Fei,et al."NOD2 in zebrafish functions in antibacterial and also antiviral responses via NF-kappa B, and also MDA5, RIG-I and MAVS".FISH & SHELLFISH IMMUNOLOGY 55(2016):173-185.
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