Optimal dietary protein level improved growth, disease resistance, intestinal immune and physical barrier function of young grass carp (Ctenopharyngodon idella)
Xu, Jing1; Wu, Pei1,2,3; Jiang, Wei-Dan1,2,3; Liu, Yang1,2,3; Jiang, Jun1,2,3; Kuang, Sheng-Yao4; Tang, Ling4; Tang, Wu-Neng4; Zhang, Yong-An5; Zhou, Xiao-Qiu1,2,3; Feng, Lin1,2,3
2016-08-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume55Pages:64-87
AbstractThis study investigated the effects of dietary proteins on the growth, disease resistance, intestinal immune and physical barrier functions of young grass carp (Ctenopharyngodon idella). A total of 540 young grass carp (264.11 +/- 0.76 g) were fed six diets containing graded levels of protein (143.1, 176.7, 217.2, 257.5, 292.2 and 322.8 g digestible protein kg(-1) diet) for 8 weeks. After the growth trial, fish were challenged with Aeromonas hydrophila and mortalities were recorded for 14 days. The results indicated that optimal dietary protein levels: increased the production of antibacterial components, up-regulated anti-inflammatory cytokines, inhibitor of kappa B alpha, target of rapamycin and ribosomal protein S6 kinases 1 mRNA levels, whereas down-regulated pro-inflammatory cytokines, nuclear factor kappa B (NF-kappa B) P65, NF-kappa B P52, c-Rel, I kappa B kinase I kappa B kinase gamma and eIF4E-binding proteins 2 mRNA levels in three intestinal segments of young grass carp (P < 0:05), suggesting that optimal dietary protein level could enhance fish intestinal immune barrier function; up-regulated the mRNA levels of tight junction complexes, B-cell lymphoma protein-2, inhibitor of apoptosis proteins, myeloid cell leukemia-1 and NF-E2-related factor 2, and increased the activities and mRNA levels of antioxidant enzymes, whereas down-regulated myosin light chain kinase, cysteinyl aspartic acid-protease 2, 3, 7, 8, 9, fatty acid synthetase ligand, apoptotic protease activating factor-1, Bcl-2 associated X protein, p38 mitogen-activated protein kinase, c-Jun N-terminal protein kinase and Kelch-like-ECH-associated protein 1b mRNA levels, and decreased reactive oxygen species, malondialdehyde and protein carbonyl contents in three intestinal segments of young grass carp (P < 0.05), indicating that optimal dietary protein level could improve fish intestinal physical barrier function. Finally, the optimal dietary protein levels for the growth performance (PWG) and against enteritis morbidity of young grass carp were estimated to be 286.82 g kg(-1) diet (250.66 g digestible protein kg(-1) diet) and 292.10 g kg(-1) diet (255.47 g digestible protein kg(-1) diet), respectively. (C) 2016 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordProtein Grass Carp (Ctenopharyngodon Idella) Disease Resistance Intestinal Immune Tight Junction Apoptosis Antioxidant
DOI10.1016/j.fsi.2016.05.021
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNational Basic Research Program of China (973 Program)(2014CB138600) ; National Basic Research Program of China (973 Program)(2014CB138600) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Science and Technology Support Programme of Sichuan Province of China(2014NZ0003) ; Science and Technology Support Programme of Sichuan Province of China(2014NZ0003) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Academy of Kechuang Feed Industry in Sichuan(2013NZ0054) ; Academy of Kechuang Feed Industry in Sichuan(2013NZ0054) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; 2013NC0045) ; 2013NC0045) ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Basic Research Program of China (973 Program)(2014CB138600) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Science and Technology Support Programme of Sichuan Province of China(2014NZ0003) ; Science and Technology Support Programme of Sichuan Province of China(2014NZ0003) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Academy of Kechuang Feed Industry in Sichuan(2013NZ0054) ; Academy of Kechuang Feed Industry in Sichuan(2013NZ0054) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; 2013NC0045) ; 2013NC0045)
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS KeywordNF-KAPPA-B ; AMINO-ACID-METABOLISM ; BREAM SPARUS-AURATA ; SALMON SALMO-SALAR ; GENE-EXPRESSION ; OXIDATIVE STRESS ; JUVENILE JIAN ; VAR. JIAN ; AEROMONAS-HYDROPHILA ; ANTIOXIDANT STATUS
WOS IDWOS:000381537000007
Funding OrganizationNational Basic Research Program of China (973 Program)(2014CB138600) ; National Basic Research Program of China (973 Program)(2014CB138600) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Science and Technology Support Programme of Sichuan Province of China(2014NZ0003) ; Science and Technology Support Programme of Sichuan Province of China(2014NZ0003) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Academy of Kechuang Feed Industry in Sichuan(2013NZ0054) ; Academy of Kechuang Feed Industry in Sichuan(2013NZ0054) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; 2013NC0045) ; 2013NC0045) ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Basic Research Program of China (973 Program)(2014CB138600) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Science and Technology Support Programme of Sichuan Province of China(2014NZ0003) ; Science and Technology Support Programme of Sichuan Province of China(2014NZ0003) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Academy of Kechuang Feed Industry in Sichuan(2013NZ0054) ; Academy of Kechuang Feed Industry in Sichuan(2013NZ0054) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; 2013NC0045) ; 2013NC0045)
Citation statistics
Cited Times:29[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/28342
Collection藻类生物学及应用研究中心_水生生物分子与细胞生物学研究中心
Affiliation1.Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Sichuan, Peoples R China
2.Sichuan Agr Univ, Fish Nutr & Safety Prod Univ Key Lab Sichuan Prov, Chengdu 611130, Sichuan, Peoples R China
3.Sichuan Agr Univ, China Minist Educ, Key Lab Anim Dis Resistance Nutr, Chengdu 611130, Sichuan, Peoples R China
4.Sichuan Acad Anim Sci, Inst Anim Nutr, Chengdu 610066, Peoples R China
5.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Xu, Jing,Wu, Pei,Jiang, Wei-Dan,et al. Optimal dietary protein level improved growth, disease resistance, intestinal immune and physical barrier function of young grass carp (Ctenopharyngodon idella)[J]. FISH & SHELLFISH IMMUNOLOGY,2016,55:64-87.
APA Xu, Jing.,Wu, Pei.,Jiang, Wei-Dan.,Liu, Yang.,Jiang, Jun.,...&Feng, Lin.(2016).Optimal dietary protein level improved growth, disease resistance, intestinal immune and physical barrier function of young grass carp (Ctenopharyngodon idella).FISH & SHELLFISH IMMUNOLOGY,55,64-87.
MLA Xu, Jing,et al."Optimal dietary protein level improved growth, disease resistance, intestinal immune and physical barrier function of young grass carp (Ctenopharyngodon idella)".FISH & SHELLFISH IMMUNOLOGY 55(2016):64-87.
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