Impact of exogenous lipase supplementation on growth, intestinal function, mucosal immune and physical barrier, and related signaling molecules mRNA expression of young grass carp (Ctenopharyngodon idella)
Liu, Sen1; Feng, Lin1,3,4; Jiang, Wei-Dan1,3,4; Liu, Yang1,3,4; Jiang, Jun1,3,4; Wu, Pei1,3,4; Zeng, Yun-Yun1,3,4; Xu, Shu-De2; Kuang, Sheng-Yao5; Tang, Ling5; Tang, Wu-Neng5; Zhang, Yong-An6; Zhou, Xiao-Qiu1,3,4
2016-08-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume55Pages:88-105
AbstractThis study investigated the effects of exogenous lipase supplementation on the growth performance, intestinal growth and function, immune response and physical barrier function, and related signaling molecules mRNA expression of young grass carp (Ctenopharyngodon idella). A total of 450 grass carp (255.02 +/- 0.34 g) were fed five diets for 60 days. There were 5 dietary treatments that included a normal protein and lipid diet containing 30% crude protein (CP) with 5% ether extract (EE), and the low-protein and high-lipid diets (28% CP, 6% EE) supplemented with graded levels of exogenous lipase supplementation activity at 0, 1193, 2560 and 3730 U/kg diet. The results indicated that compared with a normal protein and lipid diet (30% CP, 5% EE), a low-protein and high-lipid diet (28% CP, 6% EE) (un-supplemented lipase) improved lysozyme activities and complement component 3 contents in the distal intestine (DI), interleukin 10 mRNA expression in the proximal intestine (Pp, and glutathione Stransferases activity and glutathione content in the intestine of young grass carp. In addition, in low protein and high-lipid diets, optimal exogenous lipase supplementation significantly increased acid phosphatase (ACP) activities and complement component 3 (C3) contents (P < 0.05), up-regulated the relative mRNA levels of antimicrobial peptides (liver expressed antimicrobial peptide 2 and hepcidin) and anti-inflammatory cytokines (interleukin 10 and transforming growth factor 01) and signaling molecules inhibitor protein-kappa B alpha (I kappa B alpha) and target of rapamycin (TOR) (P < 0.05), down-regulated the mRNA levels of pro-inflammatory cytokines (tumor necrosis factor alpha, interleukin 8, interferon gamma 2, and interleukin 1 beta), and signaling molecules (nuclear factor kappa B p65, I kappa B kinase beta, I kappa B kinase gamma) (P < 0.05) in the intestine of young grass carp. Moreover, optimal exogenous lipase supplementation significantly decreased reactive oxygen species (ROS), malondialdehyde (MDA) and protein carbonyl (PC) contents (P < 0.05), improved the activities of anti-superoxide anion (ASA) and anti-hydroxyl radical (AHR), glutathione content, and the activities and mRNA levels of antioxidant enzymes (copper/zinc superoxide dismutase, manganese superoxide dismutase, catalase, glutathione peroxidase, glutathione S-transferases and glutathione reductase) (P < 0.05), up-regulated signaling molecule NF-E2-related factor 2 (Nrf2) (P < 0.05), down-regulated signaling molecules (Kelch-like-ECH-associated protein la, Kelch-like-ECH-associated protein 1b) (P < 0.05) in the intestine of young grass carp. Furthermore, optimal exogenous lipase supplementation significantly elevated the mRNA levels of tight junction proteins (Occludin, zonula occludens 1, Claudin b, Claudin c and Claudin 3) (P < 0.05), down-regulated the mRNA levels of tight junction proteins (Claudin 12 and Claudin 15a) (P < 0.05), down-regulated signaling molecules myosin light chain kinase (P < 0.05) in the intestine of young grass carp. In conclusion, dietary lipid could partially spare protein, and the low-protein and high-lipid diet could improve growth, intestinal growth and function, immune response and antioxidant capability of fish. Meanwhile, in high-fat and low protein diets, optimal exogenous lipase supplementation improved growth, intestinal growth and function, intestinal immunity, physical barrier, and regulated the mRNA expression of related signal molecules of fish.
SubtypeArticle
KeywordExogenous Lipase Supplementation Grass Carp (Ctenopharyngodon Idella) Immune Response Tight Junction Antioxidant Capability Intestine
DOI10.1016/j.fsi.2016.05.006
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNational Basic Research Program of China (973 Program)(2014CB138600) ; National Basic Research Program of China (973 Program)(2014CB138600) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; 2013NC0045) ; 2013NC0045) ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Basic Research Program of China (973 Program)(2014CB138600) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; 2013NC0045) ; 2013NC0045)
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS KeywordNF-KAPPA-B ; POLYUNSATURATED FATTY-ACIDS ; ANTIOXIDANT ENZYME-ACTIVITIES ; TIGHT JUNCTION PROTEINS ; SALMON SALMO-SALAR ; BODY-COMPOSITION ; JUVENILE JIAN ; VAR. JIAN ; INFLAMMATORY RESPONSE ; DISEASE RESISTANCE
WOS IDWOS:000381537000008
Funding OrganizationNational Basic Research Program of China (973 Program)(2014CB138600) ; National Basic Research Program of China (973 Program)(2014CB138600) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; 2013NC0045) ; 2013NC0045) ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Basic Research Program of China (973 Program)(2014CB138600) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; 2013NC0045) ; 2013NC0045)
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/28341
Collection藻类生物学及应用研究中心_水生生物分子与细胞生物学研究中心
Affiliation1.Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Peoples R China
2.Guangdong Vtr Biotech Co Ltd, Zhuhai 519060, Peoples R China
3.Sichuan Agr Univ, Fish Nutr & Safety Prod Univ Key Lab Sichuan Prov, Chengdu 611130, Peoples R China
4.Sichuan Agr Univ, China Minist Educ, Key Lab Anim Dis Resistance Nutr, Chengdu 611130, Peoples R China
5.Sichuan Acad Anim Sci, Inst Anim Nutr, Chengdu 610066, Peoples R China
6.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
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Liu, Sen,Feng, Lin,Jiang, Wei-Dan,et al. Impact of exogenous lipase supplementation on growth, intestinal function, mucosal immune and physical barrier, and related signaling molecules mRNA expression of young grass carp (Ctenopharyngodon idella)[J]. FISH & SHELLFISH IMMUNOLOGY,2016,55:88-105.
APA Liu, Sen.,Feng, Lin.,Jiang, Wei-Dan.,Liu, Yang.,Jiang, Jun.,...&Zhou, Xiao-Qiu.(2016).Impact of exogenous lipase supplementation on growth, intestinal function, mucosal immune and physical barrier, and related signaling molecules mRNA expression of young grass carp (Ctenopharyngodon idella).FISH & SHELLFISH IMMUNOLOGY,55,88-105.
MLA Liu, Sen,et al."Impact of exogenous lipase supplementation on growth, intestinal function, mucosal immune and physical barrier, and related signaling molecules mRNA expression of young grass carp (Ctenopharyngodon idella)".FISH & SHELLFISH IMMUNOLOGY 55(2016):88-105.
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