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A simplified method for the simultaneous detection of nervous necrosis virus and iridovirus in grouper Epinephelus spp.
Yu, N-T1,2; Zhang, Y-L1; Xiong, Z.3,4; Qu, L.1; Liu, Z-X1
Corresponding AuthorYu, N-T(yunaitong@163.com) ; Liu, Z-X(liuzhixin@itbb.org.cn)
2019
Source PublicationACTA VIROLOGICA
ISSN0001-723X
Volume63Issue:1Pages:80-87
AbstractGrouper nervous necrosis virus (GNNV) and grouper iridovirus (GIV) are major grouper-infecting viruses in southern China that can cause serious economic losses. A duplex reverse transcription-PCR (duplex RT-PCR) method was developed for the simultaneous detection of GNNV and GIV. Eight groups of primers specifically targeting the capsid protein genes of GNNV and GIV were designed and analyzed. The primer set GN4 was selected and used to amplify fragments of 887 bp and 319 bp in length from GNNV and GIV, respectively. Furthermore, the duplex PCR assay was shown to be sensitive because it could detect at least 20 pg of plasmid-viral DNA from a mixture of viruses. Using this assay, 18 GNNV infected groupers and 7 GIV infected groupers were detected amongst 41 suspected samples in Hainan. The duplex RT-PCR assay proved to be a rapid, specific, and sensitive method for detecting the two grouper viruses. This method could be used to facilitate better control of fish viruses through early detection.
Keywordduplex reverse transcription-PCR nervous necrosis virus iridovirus grouper
DOI10.4149/av_2019_107
Funding OrganizationNational Nonprofit Institute Research Grant of CATAS ; National Nonprofit Institute Research Grant of CATAS ; National Nonprofit Institute Research Grant of CATAS ; National Nonprofit Institute Research Grant of CATAS ; Major Science and Technology Program of Hainan Province ; Major Science and Technology Program of Hainan Province ; Major Science and Technology Program of Hainan Province ; Major Science and Technology Program of Hainan Province ; Young Elite Scientists Sponsorship Program by CSTC ; Young Elite Scientists Sponsorship Program by CSTC ; Young Elite Scientists Sponsorship Program by CSTC ; Young Elite Scientists Sponsorship Program by CSTC ; National Nonprofit Institute Research Grant of CATAS ; National Nonprofit Institute Research Grant of CATAS ; National Nonprofit Institute Research Grant of CATAS ; National Nonprofit Institute Research Grant of CATAS ; Major Science and Technology Program of Hainan Province ; Major Science and Technology Program of Hainan Province ; Major Science and Technology Program of Hainan Province ; Major Science and Technology Program of Hainan Province ; Young Elite Scientists Sponsorship Program by CSTC ; Young Elite Scientists Sponsorship Program by CSTC ; Young Elite Scientists Sponsorship Program by CSTC ; Young Elite Scientists Sponsorship Program by CSTC ; National Nonprofit Institute Research Grant of CATAS ; National Nonprofit Institute Research Grant of CATAS ; National Nonprofit Institute Research Grant of CATAS ; National Nonprofit Institute Research Grant of CATAS ; Major Science and Technology Program of Hainan Province ; Major Science and Technology Program of Hainan Province ; Major Science and Technology Program of Hainan Province ; Major Science and Technology Program of Hainan Province ; Young Elite Scientists Sponsorship Program by CSTC ; Young Elite Scientists Sponsorship Program by CSTC ; Young Elite Scientists Sponsorship Program by CSTC ; Young Elite Scientists Sponsorship Program by CSTC ; National Nonprofit Institute Research Grant of CATAS ; National Nonprofit Institute Research Grant of CATAS ; National Nonprofit Institute Research Grant of CATAS ; National Nonprofit Institute Research Grant of CATAS ; Major Science and Technology Program of Hainan Province ; Major Science and Technology Program of Hainan Province ; Major Science and Technology Program of Hainan Province ; Major Science and Technology Program of Hainan Province ; Young Elite Scientists Sponsorship Program by CSTC ; Young Elite Scientists Sponsorship Program by CSTC ; Young Elite Scientists Sponsorship Program by CSTC ; Young Elite Scientists Sponsorship Program by CSTC
Indexed BySCI ; SCI
Language英语
Funding ProjectNational Nonprofit Institute Research Grant of CATAS[1630052016014] ; Major Science and Technology Program of Hainan Province[ZDKJ2017003] ; Young Elite Scientists Sponsorship Program by CSTC[CSTC-QN201704]
WOS Research AreaVirology
WOS SubjectVirology
WOS IDWOS:000468126700009
WOS KeywordMULTIPLEX RT-PCR ; SPOTTED GROUPER ; BETANODAVIRUS ; PATHOGENICITY ; MONOPLEX ; SJNNV
PublisherAEPRESS SRO
Funding OrganizationNational Nonprofit Institute Research Grant of CATAS ; National Nonprofit Institute Research Grant of CATAS ; National Nonprofit Institute Research Grant of CATAS ; National Nonprofit Institute Research Grant of CATAS ; Major Science and Technology Program of Hainan Province ; Major Science and Technology Program of Hainan Province ; Major Science and Technology Program of Hainan Province ; Major Science and Technology Program of Hainan Province ; Young Elite Scientists Sponsorship Program by CSTC ; Young Elite Scientists Sponsorship Program by CSTC ; Young Elite Scientists Sponsorship Program by CSTC ; Young Elite Scientists Sponsorship Program by CSTC ; National Nonprofit Institute Research Grant of CATAS ; National Nonprofit Institute Research Grant of CATAS ; National Nonprofit Institute Research Grant of CATAS ; National Nonprofit Institute Research Grant of CATAS ; Major Science and Technology Program of Hainan Province ; Major Science and Technology Program of Hainan Province ; Major Science and Technology Program of Hainan Province ; Major Science and Technology Program of Hainan Province ; Young Elite Scientists Sponsorship Program by CSTC ; Young Elite Scientists Sponsorship Program by CSTC ; Young Elite Scientists Sponsorship Program by CSTC ; Young Elite Scientists Sponsorship Program by CSTC ; National Nonprofit Institute Research Grant of CATAS ; National Nonprofit Institute Research Grant of CATAS ; National Nonprofit Institute Research Grant of CATAS ; National Nonprofit Institute Research Grant of CATAS ; Major Science and Technology Program of Hainan Province ; Major Science and Technology Program of Hainan Province ; Major Science and Technology Program of Hainan Province ; Major Science and Technology Program of Hainan Province ; Young Elite Scientists Sponsorship Program by CSTC ; Young Elite Scientists Sponsorship Program by CSTC ; Young Elite Scientists Sponsorship Program by CSTC ; Young Elite Scientists Sponsorship Program by CSTC ; National Nonprofit Institute Research Grant of CATAS ; National Nonprofit Institute Research Grant of CATAS ; National Nonprofit Institute Research Grant of CATAS ; National Nonprofit Institute Research Grant of CATAS ; Major Science and Technology Program of Hainan Province ; Major Science and Technology Program of Hainan Province ; Major Science and Technology Program of Hainan Province ; Major Science and Technology Program of Hainan Province ; Young Elite Scientists Sponsorship Program by CSTC ; Young Elite Scientists Sponsorship Program by CSTC ; Young Elite Scientists Sponsorship Program by CSTC ; Young Elite Scientists Sponsorship Program by CSTC
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/28104
Collection其他
Corresponding AuthorYu, N-T; Liu, Z-X
Affiliation1.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou 571101, Hainan, Peoples R China
2.Univ Chinese Acad Sci, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
3.Univ Arizona, Sch Plant Sci, Tucson, AZ 85721 USA
4.Univ Arizona, BIO5 Inst, Tucson, AZ 85721 USA
Recommended Citation
GB/T 7714
Yu, N-T,Zhang, Y-L,Xiong, Z.,et al. A simplified method for the simultaneous detection of nervous necrosis virus and iridovirus in grouper Epinephelus spp.[J]. ACTA VIROLOGICA,2019,63(1):80-87.
APA Yu, N-T,Zhang, Y-L,Xiong, Z.,Qu, L.,&Liu, Z-X.(2019).A simplified method for the simultaneous detection of nervous necrosis virus and iridovirus in grouper Epinephelus spp..ACTA VIROLOGICA,63(1),80-87.
MLA Yu, N-T,et al."A simplified method for the simultaneous detection of nervous necrosis virus and iridovirus in grouper Epinephelus spp.".ACTA VIROLOGICA 63.1(2019):80-87.
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