IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心
SVCV infection triggers fish IFN response through RLR signaling pathway
Gong, Xiu-Ying1,2; Zhang, Qi-Min1,2; Gui, Jian-Fang1,2; Zhang, Yi-Bing1,2
Corresponding AuthorZhang, Yi-Bing(ybzhang@ihb.ac.cn)
2019-03-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume86Pages:1058-1063
AbstractIn mammals, virus infection of host cells triggers innate immune response, characterized by induction of interferon (IFN) and downstream IFN-stimulated genes (ISGs). The initiation of IFN antiviral response is dependent on host recognition of virus infection. In fish, similar IFN antiviral response is induced in response to RNA or DNA virus infection; however, the detailed mechanisms underlying recognition of a given virus and activation of downstream signaling remain largely unexplored. Using an infection model with Epithelioma papulosum cyprini (EPC) cells and spring viremia of carp virus (SVCV), a negative sense single-stranded RNA virus, we reported that fish RLR signaling pathway was involved in SVCV-triggered fish IFN response. IFN response was significantly initiated in EPC cells when infected with SVCV, as evidenced by activation of fish IFN promoters, upregulation of IFN and ISGs at mRNA and protein levels. However, function blockade of RIG-I and MDA5, two cytosolic receptors of fish RLR family, significantly attenuated the activation of fish IFN promoters and also the induction of fish IFN and ISGs by SVCV infection. Consistently, SVCV infection-triggered IFN response were blocked in EPC cells when transfected with the dominant negative mutants of pivotal RLR signaling factors, including MAVS, MITA, TBK1, IRF3 and IRF7. These results together shed light on the conservation of RLR-mediated IFN signaling that contributes to fish cells responding to RNA virus infection.
KeywordRNA virus infection Fish IFN response RLR receptor Signaling pathway
DOI10.1016/j.fsi.2018.12.063
Funding OrganizationNational Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
Indexed BySCI ; SCI
Language英语
Funding ProjectNational Key R&D Program of China[2018YFD0900302] ; National Natural Science Foundation of China[31572646] ; National Natural Science Foundation of China[31772875]
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000459528400117
WOS KeywordANTIVIRAL RESPONSE ; RIG-I ; SPRING VIREMIA ; ZEBRAFISH IRF1 ; RECEPTORS ; BINDING ; PROTEIN ; EXPRESSION ; IFN-PHI-1 ; REVEALS
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Funding OrganizationNational Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/28060
Collection鱼类生物学及渔业生物技术研究中心
Corresponding AuthorZhang, Yi-Bing
Affiliation1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Key Lab Aquaculture Dis Control, Minist Agr,Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 10049, Peoples R China
Recommended Citation
GB/T 7714
Gong, Xiu-Ying,Zhang, Qi-Min,Gui, Jian-Fang,et al. SVCV infection triggers fish IFN response through RLR signaling pathway[J]. FISH & SHELLFISH IMMUNOLOGY,2019,86:1058-1063.
APA Gong, Xiu-Ying,Zhang, Qi-Min,Gui, Jian-Fang,&Zhang, Yi-Bing.(2019).SVCV infection triggers fish IFN response through RLR signaling pathway.FISH & SHELLFISH IMMUNOLOGY,86,1058-1063.
MLA Gong, Xiu-Ying,et al."SVCV infection triggers fish IFN response through RLR signaling pathway".FISH & SHELLFISH IMMUNOLOGY 86(2019):1058-1063.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Gong, Xiu-Ying]'s Articles
[Zhang, Qi-Min]'s Articles
[Gui, Jian-Fang]'s Articles
Baidu academic
Similar articles in Baidu academic
[Gong, Xiu-Ying]'s Articles
[Zhang, Qi-Min]'s Articles
[Gui, Jian-Fang]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Gong, Xiu-Ying]'s Articles
[Zhang, Qi-Min]'s Articles
[Gui, Jian-Fang]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.