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题名: Molecular and functional characterization of peptidoglycan-recognition protein SC2 (PGRP-SC2) from Nile tilapia (Oreochromis niloticus) involved in the immune response to Streptococcus agalactiae
作者: Gan, Zhen1, 2, 3; Chen, Shannan1, 3; Hou, Jing1, 3; Huo, Huijun1, 3; Zhang, Xiaolin1, 3; Ruan, Baiye1, 3; Laghari, Zubair Ahmed1; Li, Li1, 3; Lu, Yishan2; Nie, Pin1, 3
关键词: Oreochromis niloticus ; Peptidoglycan-recognition protein ; Streptococcus agalactiae ; Amidase activity ; Antimicrobial effect
刊名: FISH & SHELLFISH IMMUNOLOGY
发表日期: 2016-07-01
DOI: 10.1016/j.fsi.2016.03.158
卷: 54, 页:1-10
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
英文摘要: PGRP-SC2, the member of PGRP family, plays an important role in regulation of innate immune response. In this paper, a PGRP-SC2 gene of Nile tilapia, Oreochromis niloticus (designated as On-PGRP-SC2) was cloned and its expression pattern under the infection of Streptococcus agalactiae was investigated. Sequence analysis showed main structural features required for amidase activity were detected in the deduced amino acid sequence of On-PGRP-SC2. In healthy tilapia, the On-PGRP-SC2 transcripts could be detected in all the examined tissues, with the most abundant expression in the muscle. When infected with S. agalactiae, there was a clear time-dependent expression pattern of On-PGRP-SC2 in the spleen, head kidney and brain. The assays for the amidase activity suggested that recombinant On-PGRP-SC2 protein had a Zn2+-dependent PGN-degrading activity. Moreover, our works showed that recombinant On-PGRP-SC2 protein could significantly reduce bacterial load in target organs attacked by S. agalactiae. These findings indicated that On-PGRP-SC2 may play important roles in the immune response to S. agalactiae in Nile tilapia. (C) 2016 Elsevier Ltd. All rights reserved.
关键词[WOS]: CARP CTENOPHARYNGODON-IDELLA ; GRAM-POSITIVE BACTERIA ; ANGSTROM RESOLUTION ; CRYSTAL-STRUCTURE ; EXPRESSION ; FISH ; INFECTION ; AMIDASE ; HISTORY ; PGRPS
语种: 英语
项目资助者: National Natural Science Foundation of China(31320103913)
WOS记录号: WOS:000378451700001
ISSN号: 1050-4648
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/28012
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Guangdong Ocean Univ, Coll Fishery, 40 East Jiefang Rd, Zhanjiang 524025, Guangdong, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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