IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心
Comprehensive identification and profiling of Nile tilapia (Oreochromis niloticus) microRNAs response to Streptococcus agalactiae infection through high-throughput sequencing
Wang, Bei1; Gan, Zhen1,2; Cai, Shuanghu1; Wang, Zhongliang1; Yu, Dapeng1; Lin, Ziwei1; Lu, Yishan1; Wu, Zaohe1; Jian, Jichang1
2016-07-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume54Pages:93-106
AbstractMicroRNAs are a kind of small non-coding RNAs that participate in various biological processes. Deregulated microRNA expression is associated with several types of diseases. Tilapia (Oreochromis niloticus) is an important commercial fish species in China. To identify miRNAs and investigate immune related miRNAs of O. niloticus, we applied high-throughput sequencing technology to identify and analyze miRNAs from tilapia infected with Streptococcus agalactiae at a timescale of 72 h divided into six different time points. The results showed that a total of 3009 tilapia miRNAs were identified, including in 1121 miRNAs which have homologues in the currently available databases and 1878 novel miRNAs. The expression levels of 218 tilapia miRNAs were significantly altered at 6 h-72 h post-bacterial infection (pi), and these miRNAs were therefore classified as differentially expressed tilapia miRNAs. For the 1121 differentially expressed tilapia miRNAs target 41961 genes. GO and KEGG enrichment analysis revealed that some target genes of tilapia miRNAs were grouped mainly into the categories of apoptotic process, signal pathway, and immune response. This is the first report of comprehensive identification of O. niloticus miRNAs being differentially regulated in spleen in normal conditions relating to S. agalactiae infection. This work provides an opportunity for further understanding of the molecular mechanisms of miRNA regulation in O. niloticus host-pathogen interactions. (C) 2016 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordOreochromis Niloticus Microrna Streptococcus Agalactiae Deep Sequencing Immune Response
DOI10.1016/j.fsi.2016.03.159
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNational Natural Science Foundation of China(31302226) ; National Natural Science Foundation of China(31302226) ; Science & Technology Planning Project of Guangdong Province of China(2013B020307014) ; Science & Technology Planning Project of Guangdong Province of China(2013B020307014) ; Project of Enhancing School with Innovation(HDYQ2015001) ; Project of Enhancing School with Innovation(HDYQ2015001) ; National Natural Science Foundation of China(31302226) ; National Natural Science Foundation of China(31302226) ; Science & Technology Planning Project of Guangdong Province of China(2013B020307014) ; Science & Technology Planning Project of Guangdong Province of China(2013B020307014) ; Project of Enhancing School with Innovation(HDYQ2015001) ; Project of Enhancing School with Innovation(HDYQ2015001)
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS KeywordHELICOBACTER-PYLORI INFECTION ; GROUP-B STREPTOCOCCI ; IMMUNE-RESPONSE ; T-CELLS ; MOLECULAR CHARACTERIZATION ; INFLAMMATORY RESPONSE ; CONSERVED MICRORNAS ; BACTERIAL-INFECTION ; INTERFERON-GAMMA ; TELEOST FISH
WOS IDWOS:000378451700012
Funding OrganizationNational Natural Science Foundation of China(31302226) ; National Natural Science Foundation of China(31302226) ; Science & Technology Planning Project of Guangdong Province of China(2013B020307014) ; Science & Technology Planning Project of Guangdong Province of China(2013B020307014) ; Project of Enhancing School with Innovation(HDYQ2015001) ; Project of Enhancing School with Innovation(HDYQ2015001) ; National Natural Science Foundation of China(31302226) ; National Natural Science Foundation of China(31302226) ; Science & Technology Planning Project of Guangdong Province of China(2013B020307014) ; Science & Technology Planning Project of Guangdong Province of China(2013B020307014) ; Project of Enhancing School with Innovation(HDYQ2015001) ; Project of Enhancing School with Innovation(HDYQ2015001)
Citation statistics
Cited Times:20[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/28009
Collection鱼类生物学及渔业生物技术研究中心
Affiliation1.Guangdong Ocean Univ, Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Key Lab Control Dis Aquat Anim, Guangdong Higher Educ Insititutes,Coll Fishery, Zhanjiang 524025, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Wang, Bei,Gan, Zhen,Cai, Shuanghu,et al. Comprehensive identification and profiling of Nile tilapia (Oreochromis niloticus) microRNAs response to Streptococcus agalactiae infection through high-throughput sequencing[J]. FISH & SHELLFISH IMMUNOLOGY,2016,54:93-106.
APA Wang, Bei.,Gan, Zhen.,Cai, Shuanghu.,Wang, Zhongliang.,Yu, Dapeng.,...&Jian, Jichang.(2016).Comprehensive identification and profiling of Nile tilapia (Oreochromis niloticus) microRNAs response to Streptococcus agalactiae infection through high-throughput sequencing.FISH & SHELLFISH IMMUNOLOGY,54,93-106.
MLA Wang, Bei,et al."Comprehensive identification and profiling of Nile tilapia (Oreochromis niloticus) microRNAs response to Streptococcus agalactiae infection through high-throughput sequencing".FISH & SHELLFISH IMMUNOLOGY 54(2016):93-106.
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