IHB OpenIR
Cloning and characterization of the LEF/TCF gene family in grass carp (Ctenopharyngodon idella) and their expression profiles in response to grass carp reovirus infection
Zhu, Denghui1,2; Huang, Rong1; Chen, Liangming1,2; Fu, Peipei1,2; Luo, Lifei1,2; He, Libo1; Li, Yongming1; Liao, Lanjie1; Zhu, Zuoyan1; Wang, Yaping1
Corresponding AuthorWang, Yaping(wangyp.ihb@gmail.com)
2019-03-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume86Pages:335-346
AbstractT-cell factor/lymphoid enhancer-binding factor (TCF/LEF) proteins from the High Mobility Group (HMG) box family act as the main downstream effectors of the Wnt signaling pathway. HMGB proteins play multifaceted roles in the immune system of mammals. To clarify the immunological characteristics of LEF/TCF genes in grass carp (Ctenopharyngodon idella), five LEF/TCF genes (TCF7, LEF1, TCF7L1A, TCF7L1B, and TCF7L2) were identified and characterized. All five LEF/TCF proteins contained two characteristic domains: a HMG-BOX domain and a CTNNB1_binding region. Phylogenetic tree analysis revealed that the LEF/TCF proteins were represented different lineages. These results of subcellular localization showed that four of the LEF/TCF genes were localized exclusively within the nucleus, while TCF7L2 was localized in the cytoplasm and nucleus. The mRNA expression profiles of these LEF/TCF family genes differed across different tissues. The mRNA expression levels of TCF7, TCF7L1A, and TCF7L2 changed significantly in liver after grass carp reovirus (GCRV) challenge; TCF7 and TCF7L1A responded early while TCF7L2 responded late. This suggests that these genes may participate in GCRV-related immune responses. Moreover, TCF7 promoted Bc16 transcription in response to the GCRV challenge. These findings further our understanding of the function of LEF/TCF genes in teleosts.
KeywordGrass carp Reovirus infection LEF/TCF HMG Subcellular localization mRNA expression Immune
DOI10.1016/j.fsi.2018.11.057
Funding OrganizationNational Natural Science Foundation of China ; Strategic Priority Research Program of Chinese Academy of Sciences ; National Natural Science Foundation of China ; Strategic Priority Research Program of Chinese Academy of Sciences
Indexed BySCI ; SCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31572614] ; Strategic Priority Research Program of Chinese Academy of Sciences[XDA08030203]
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000459528400039
WOS KeywordHMGB PROTEINS ; TRANSCRIPTION FACTORS ; INNATE IMMUNITY ; WNT ; DNA ; LOCALIZATION ; CELLS ; DOMAINS ; PATTERN ; TCF7L2
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Funding OrganizationNational Natural Science Foundation of China ; Strategic Priority Research Program of Chinese Academy of Sciences ; National Natural Science Foundation of China ; Strategic Priority Research Program of Chinese Academy of Sciences
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/28003
Corresponding AuthorWang, Yaping
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Zhu, Denghui,Huang, Rong,Chen, Liangming,et al. Cloning and characterization of the LEF/TCF gene family in grass carp (Ctenopharyngodon idella) and their expression profiles in response to grass carp reovirus infection[J]. FISH & SHELLFISH IMMUNOLOGY,2019,86:335-346.
APA Zhu, Denghui.,Huang, Rong.,Chen, Liangming.,Fu, Peipei.,Luo, Lifei.,...&Wang, Yaping.(2019).Cloning and characterization of the LEF/TCF gene family in grass carp (Ctenopharyngodon idella) and their expression profiles in response to grass carp reovirus infection.FISH & SHELLFISH IMMUNOLOGY,86,335-346.
MLA Zhu, Denghui,et al."Cloning and characterization of the LEF/TCF gene family in grass carp (Ctenopharyngodon idella) and their expression profiles in response to grass carp reovirus infection".FISH & SHELLFISH IMMUNOLOGY 86(2019):335-346.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhu, Denghui]'s Articles
[Huang, Rong]'s Articles
[Chen, Liangming]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhu, Denghui]'s Articles
[Huang, Rong]'s Articles
[Chen, Liangming]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhu, Denghui]'s Articles
[Huang, Rong]'s Articles
[Chen, Liangming]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.