IHB OpenIR  > 水环境工程研究中心
Adverse Effects, Expression of the Bk-CYP3045C1 Gene, and Activation of the ERK Signaling Pathway in the Water Accommodated Fraction-Exposed Rotifer
Won, Eun-Ji1,2; Kim, Ryeo-Ok1; Kang, Hye-Min1; Kim, Hui-Su1; Hwang, Dae-Sik1; Han, Jeonghoon1; Lee, Young Hwan1; Hwang, Un-Ki3; Zhou, Bingsheng4; Lee, Su-Jae5; Lee, Jae-Seong1
2016-06-07
Source PublicationENVIRONMENTAL SCIENCE & TECHNOLOGY
ISSN0013-936X
Volume50Issue:11Pages:6025-6035
AbstractTo examine the deleterious effects of the water accommodated fraction (WAF) of crude oil, the growth curve, fecundity, and lifespan of the monogonont rotifer (Brachionus koreanus) were measured for 24 h in response to three different doses (0.2X, 0.4X, and 0.8X) of WAFs. A higher dose of WAFs significantly reduced the fecundity and lifespan. A rotifer 32K microarray chip showed that the Bk-CYP3045C1 gene had the highest expression. Of the 25 entire CYP genes, the Bk-CYP3045C1 gene showed a significant expression for different doses and times in response to WAFs and chemical components of WAFs (naphthalene and phenanthrene); also, glutathione S-transferase genes, ABC transporter, and other genes showed dose responses upon exposure to 80% WAF over time. Different doses of WAFs increased the oxidative stress with an induction of reactive oxygen species (ROS) and a depletion of glutathione (GSH). Exposure to WAFs did not show toxic effects on survivability in B. koreanus; however, toxicity to WAFs was shown when piperonyl butoxide, a potent inhibitor of cytochrome P450 (CYP) enzymes, was added. This toxicity was dose-dependent. After WAFs exposure, PERK was activated over time in response to WAFs, which suggests that WAFs can be activated by the p-ERK signaling pathway.
SubtypeArticle
DOI10.1021/acs.est.6b01306
WOS HeadingsScience & Technology ; Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationMinistry of Oceans and Fisheries, Korea(PM15050 ; Ministry of Oceans and Fisheries, Korea(PM15050 ; National Fisheries Research and Development Institute(R2016032) ; National Fisheries Research and Development Institute(R2016032) ; 20150342) ; 20150342) ; Ministry of Oceans and Fisheries, Korea(PM15050 ; Ministry of Oceans and Fisheries, Korea(PM15050 ; National Fisheries Research and Development Institute(R2016032) ; National Fisheries Research and Development Institute(R2016032) ; 20150342) ; 20150342)
Language英语
WOS Research AreaEngineering ; Environmental Sciences & Ecology
WOS SubjectEngineering, Environmental ; Environmental Sciences
WOS KeywordPOLYCYCLIC AROMATIC-HYDROCARBONS ; CRUDE-OIL ; BRACHIONUS-KOREANUS ; MONOGONONT ROTIFER ; CYTOCHROME-P450 CYP ; OXIDATIVE STRESS ; PROTEIN-KINASES ; DISPERSED OIL ; TOXICITY ; SPILL
WOS IDWOS:000377629900072
Funding OrganizationMinistry of Oceans and Fisheries, Korea(PM15050 ; Ministry of Oceans and Fisheries, Korea(PM15050 ; National Fisheries Research and Development Institute(R2016032) ; National Fisheries Research and Development Institute(R2016032) ; 20150342) ; 20150342) ; Ministry of Oceans and Fisheries, Korea(PM15050 ; Ministry of Oceans and Fisheries, Korea(PM15050 ; National Fisheries Research and Development Institute(R2016032) ; National Fisheries Research and Development Institute(R2016032) ; 20150342) ; 20150342)
Citation statistics
Cited Times:15[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/28000
Collection水环境工程研究中心
Affiliation1.Sungkyunkwan Univ SKKU, Coll Sci, Dept Biol Sci, Suwon 16419, South Korea
2.Korea Inst Ocean Sci & Technol, Marine Chem & Geochem Res Ctr, Ansan 15627, South Korea
3.Natl Fisheries Res & Dev Inst, West Sea Fisheries Res Inst, Marine Ecol Risk Assessment Ctr, Inchon 22383, South Korea
4.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
5.Hanyang Univ, Dept Life Sci, Coll Nat Sci, Seoul 04763, South Korea
Recommended Citation
GB/T 7714
Won, Eun-Ji,Kim, Ryeo-Ok,Kang, Hye-Min,et al. Adverse Effects, Expression of the Bk-CYP3045C1 Gene, and Activation of the ERK Signaling Pathway in the Water Accommodated Fraction-Exposed Rotifer[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2016,50(11):6025-6035.
APA Won, Eun-Ji.,Kim, Ryeo-Ok.,Kang, Hye-Min.,Kim, Hui-Su.,Hwang, Dae-Sik.,...&Lee, Jae-Seong.(2016).Adverse Effects, Expression of the Bk-CYP3045C1 Gene, and Activation of the ERK Signaling Pathway in the Water Accommodated Fraction-Exposed Rotifer.ENVIRONMENTAL SCIENCE & TECHNOLOGY,50(11),6025-6035.
MLA Won, Eun-Ji,et al."Adverse Effects, Expression of the Bk-CYP3045C1 Gene, and Activation of the ERK Signaling Pathway in the Water Accommodated Fraction-Exposed Rotifer".ENVIRONMENTAL SCIENCE & TECHNOLOGY 50.11(2016):6025-6035.
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