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Respiratory toxicity of cyanobacterial aphantoxins from Aphanizomenon flos-aquae DC-1 in the zebrafish gill
Zhang, De Lu1; Liu, Si Yi1; Zhang, Jing2; Zhang, Jian Kun1; Hu, Chun Xiang3; Liu, Yong Ding3
2016-07-01
Source PublicationAQUATIC TOXICOLOGY
ISSN0166-445X
Volume176Pages:106-115
AbstractAphantoxins from Aphanizomenon flos-aquae are frequently identified in eutrophic waterbodies worldwide. These toxins severely endanger environmental safety and human health due to the production of paralytic shellfish poisons (PSPs). Although the molecular mechanisms of aphantoxin neurotoxicity have been studied, many questions remain to be resolved such as in vivo alterations in branchial histology and neurotransmitter inactivation induced by these neurotoxins. Aphantoxins extracted from a naturally isolated strain of A.flos-aquae DC-1 were determined by high performance liquid chromatography. The basic components of the isolated aphantoxins identified were gonyautoxin 1 (GTX1), gonyautoxin 5 (GTX5), and neosaxitoxin (neoSTX), which comprised 34.04, 21.28, and 12.77% of the total, respectively. Zebrafish (Danio rerio) was administrated 53 or 7.61 mg STX equivalents (eq)/kg (low and high doses, respectively) of the A. flos-aquae DC-1 aphantoxins by intraperitoneal injection. Histological alterations and changes in neurotransmitter inactivation in the gills of zebrafish were investigated for 24 h following exposure. Aphantoxin exposure significantly increased the activities of gill alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and resulted in histological alterations in the gills during the first 12 h of exposure, indicating the induction of functional and structural damage. Gill acetylcholinesterase (AChE) and monoamine oxidase (MAO) activities were inhibited significantly, suggesting an alteration of neurotransmitter inactivation in zebrafish gills. The observed alterations in gill structure and function followed a time- and dose-dependent pattern. The results demonstrate that aphantoxins or PSPs lead to structural damage and altered function in the gills of zebrafish, including changes in histological structure and increases in the activities of AST and ALT. The inhibition of the activities of AChE and MAO suggest that aphantoxins or PSPs could induce respiratory toxicity in the zebrafish gill. Furthermore, these parameters may be used as bioindicators for investigating aphantoxin exposure and cyanobacterial blooms in nature. (C) 2016 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordAphantoxins Ache Histological Alteration Mao Respiratory Toxicity Zebrafish Gill
DOI10.1016/j.aquatox.2016.04.014
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationStudents Independent Innovation Program of Wuhan University of Technology(2015-HS-B1-11) ; Students Independent Innovation Program of Wuhan University of Technology(2015-HS-B1-11) ; National High-Tech Research and Development Program of China(2013AA065804) ; National High-Tech Research and Development Program of China(2013AA065804) ; Students Independent Innovation Program of Wuhan University of Technology(2015-HS-B1-11) ; Students Independent Innovation Program of Wuhan University of Technology(2015-HS-B1-11) ; National High-Tech Research and Development Program of China(2013AA065804) ; National High-Tech Research and Development Program of China(2013AA065804)
Language英语
WOS Research AreaMarine & Freshwater Biology ; Toxicology
WOS SubjectMarine & Freshwater Biology ; Toxicology
WOS KeywordCARP CYPRINUS-CARPIO ; HISTOPATHOLOGICAL CHANGES ; PIARACTUS-MESOPOTAMICUS ; OREOCHROMIS-NILOTICUS ; PROCHILODUS-LINEATUS ; ENZYME-ACTIVITIES ; PSP TOXINS ; LIVER ; FISH ; EXPOSURE
WOS IDWOS:000377734500012
Funding OrganizationStudents Independent Innovation Program of Wuhan University of Technology(2015-HS-B1-11) ; Students Independent Innovation Program of Wuhan University of Technology(2015-HS-B1-11) ; National High-Tech Research and Development Program of China(2013AA065804) ; National High-Tech Research and Development Program of China(2013AA065804) ; Students Independent Innovation Program of Wuhan University of Technology(2015-HS-B1-11) ; Students Independent Innovation Program of Wuhan University of Technology(2015-HS-B1-11) ; National High-Tech Research and Development Program of China(2013AA065804) ; National High-Tech Research and Development Program of China(2013AA065804)
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/27993
Collection藻类生物学及应用研究中心
Affiliation1.Wuhan Univ Technol, Coll Chem Chem Engn & Life Sci, Dept Lifesci & Biotechnol, Wuhan 430070, Peoples R China
2.Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Zhang, De Lu,Liu, Si Yi,Zhang, Jing,et al. Respiratory toxicity of cyanobacterial aphantoxins from Aphanizomenon flos-aquae DC-1 in the zebrafish gill[J]. AQUATIC TOXICOLOGY,2016,176:106-115.
APA Zhang, De Lu,Liu, Si Yi,Zhang, Jing,Zhang, Jian Kun,Hu, Chun Xiang,&Liu, Yong Ding.(2016).Respiratory toxicity of cyanobacterial aphantoxins from Aphanizomenon flos-aquae DC-1 in the zebrafish gill.AQUATIC TOXICOLOGY,176,106-115.
MLA Zhang, De Lu,et al."Respiratory toxicity of cyanobacterial aphantoxins from Aphanizomenon flos-aquae DC-1 in the zebrafish gill".AQUATIC TOXICOLOGY 176(2016):106-115.
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