IHB OpenIR
The adverse effect of TCIPP and TCEP on neurodevelopment of zebrafish embryos/larvae
Li, Ruiwen1,2,3; Wang, Hengqi1,2; Mi, Chuang4; Feng, Chenglian5; Zhang, Ling1,2; Yang, Lihua3; Zhou, Bingsheng3
Corresponding AuthorZhang, Ling(zhangling@wust.edu.cn) ; Yang, Lihua(lhyang@ihb.ac.cn)
2019-04-01
Source PublicationCHEMOSPHERE
ISSN0045-6535
Volume220Pages:811-817
AbstractTris (1-chloro-2-propyl) phosphate (TCIPP) and tris (2-chloroethyl)phosphate (TCEP) are two widely used chlorinated organophosphate flame retardants (ClOPFRs), and have been frequently detected in various environmental media. Concern is now growing whether TCIPP and TCEP can cause neurotoxicity since they have similar chemical structure with organophosphorus pesticide. Therefore, in this study, zebrafish embryos (2-120 h post-fertilization [hpf]) were exposed to TCIPP or TCEP (0, 100, 500 or 2500 mu g/L) or a model neurotoxicant, chlorpyrifos (CPF, 100 mu g/L) to investigate the adverse effects and possible mechanisms of TCIPP and TCEP on neurodevelopment. Our results showed that CPF exposure resulted in developmental toxicity including decreased hatching, survival rates and increased malformation rates (e.g., spinal curvature) as well as behavior changes such as decreased locomotive activity in dark stimulation. In contrast, TCIPP and TCEP showed no significant effects on developmental parameters, but caused similar effects on locomotive activity at high concentration, indicating that although not as potent as CPF. TCIPP and TCEP may still cause adverse effects on neurodevelopment. Furthermore, our results suggest that TCIPP and TCEP showed no effects on acetylcholine content or AChE activity, which were considered as the main targets of CPF. However, TCIPP and TCEP exposure can significantly down regulate the expression of selected genes and proteins related to neurodevelopment (e.g., mbp, syn2a, and alpha 1-tubulin) similar as CPF did. Besides that, TCIPP and TCEP can also affect the transcription of shha and gap43, which were not affected by CPF, pointing out a complex mechanism underlying TCIPP and TCEP's neurodevelopmental toxicity. Overall, our results demonstrated that TCEP and TCIPP may have adverse effect on the neurodevelopment of zebrafish embryos/larvae, but the underlying mechanism is not via the inhibition of acetyl cholinesterase activity. (C) 2018 Published by Elsevier Ltd.
KeywordTCEP TCIPP Neurodevelopment Zebrafish Acetylcholin esterase
DOI10.1016/j.chemosphere.2018.12.198
Funding OrganizationChina Postdoctoral Science Foundation ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; China Postdoctoral Science Foundation ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences
Indexed BySCI ; SCI
Language英语
Funding ProjectChina Postdoctoral Science Foundation[2018M630892] ; National Natural Science Foundation of China[21737005] ; National Natural Science Foundation of China[41773085] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB14040103]
WOS Research AreaEnvironmental Sciences & Ecology
WOS SubjectEnvironmental Sciences
WOS IDWOS:000458591200090
WOS KeywordORGANOPHOSPHATE FLAME RETARDANTS ; DEVELOPMENTAL NEUROTOXICITY ; PHOSPHATE TDCPP ; EXPOSURE ; NEUROTRANSMITTERS ; PLASTICIZERS ; PROTEIN ; METABOLISM ; EXPRESSION ; PROFILES
PublisherPERGAMON-ELSEVIER SCIENCE LTD
Funding OrganizationChina Postdoctoral Science Foundation ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; China Postdoctoral Science Foundation ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/27978
Corresponding AuthorZhang, Ling; Yang, Lihua
Affiliation1.Wuhan Univ Sci & Technol, Sch Publ Hlth, Dept Nutr & Toxicol, Wuhan, Hubei, Peoples R China
2.Wuhan Univ Sci & Technol, Hubei Prov Key Lab Occupat Hazard Identificat & C, Wuhan, Hubei, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
4.China Three Gorges Corp, Beijing 100012, Peoples R China
5.Chinese Res Inst Environm Sci, State Key Lab Environm Criteria & Risk Assessment, Beijing 100012, Peoples R China
Recommended Citation
GB/T 7714
Li, Ruiwen,Wang, Hengqi,Mi, Chuang,et al. The adverse effect of TCIPP and TCEP on neurodevelopment of zebrafish embryos/larvae[J]. CHEMOSPHERE,2019,220:811-817.
APA Li, Ruiwen.,Wang, Hengqi.,Mi, Chuang.,Feng, Chenglian.,Zhang, Ling.,...&Zhou, Bingsheng.(2019).The adverse effect of TCIPP and TCEP on neurodevelopment of zebrafish embryos/larvae.CHEMOSPHERE,220,811-817.
MLA Li, Ruiwen,et al."The adverse effect of TCIPP and TCEP on neurodevelopment of zebrafish embryos/larvae".CHEMOSPHERE 220(2019):811-817.
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