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Oral vaccination of tilapia against Streptococcus agalactiae using Bacillus subtilis spores expressing Sip
Yao, Yuan-Yuan1,4; Chen, Dan-Dan1; Cui, Zheng-Wei1,4; Zhang, Xiang-Yang1,4; Zhou, Yuan-Yuan1,4; Guo, Xia5; Li, Ai-Hua1,6; Zhang, Yong-An1,2,3
Corresponding AuthorLi, Ai-Hua(liaihua@ihb.ac.cn) ; Zhang, Yong-An(yzhang@ihb.ac.cn)
2019-03-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume86Pages:999-1008
AbstractStreptococcus agalactiae infections are becoming an increasing problem in aquaculture because of significant morbidity and mortality, which restricts the healthy development of tilapia aquaculture. To seek safe and effective prevention measures, a Bacillus subtilis GC5 surface displayed vaccine was prepared and applied orally in tilapia. The study first showed that recombinant spores can engraft in the tilapia intestine. Then, the effect of protection and the immune responses were evaluated. The results of ELISA showed that Sip-specific antibody in the sera of GC5-Sip-immunized fish can be detected after the first oral administration when compared to the phosphate buffer saline (PBS) control group, and the levels of specific IgM gradually strengthened with boosting, so does the specific antibody against bacteria, proving that humoral immunity was induced. Quantitative real-time PCR (qRT-PCR) results showed that the immune-related gene expression of the gut and spleen exhibited a different rising trend in the GC5-Sip group, revealing that innate immune response and local as well as systemic cellular immunity were induced. The outcome of fish immunized with GC5-Sip spores provided a relative percent survival (RPS) of 41.7% against S. agalactiae and GC5 group had an RPS of 24.2%, indicating that GC5-Sip was safe and effective in protecting tilapia against bacterial infection. Our study demonstrated that the oral administration of B. subtilis spores expressing Sip could cause an effective immune response and offer good resistance to bacterial infection. Our work may lead to the development of new ideas for immunoprophylaxis against S. agalactiae infection.
KeywordOreochromis niloticus Bacterial infection Vaccine Bacillus Surface display
DOI10.1016/j.fsi.2018.12.060
Funding OrganizationState Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology
Indexed BySCI ; SCI
Language英语
Funding ProjectState Key Laboratory of Freshwater Ecology and Biotechnology[2016FBZ04]
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000459528400110
WOS KeywordOREOCHROMIS-NILOTICUS ; ATLANTIC SALMON ; NILE TILAPIA ; TRANSCRIPTION FACTOR ; CLONORCHIS-SINENSIS ; IMMUNE-RESPONSE ; FISH ; IMMUNOGENICITY ; RESISTANCE ; PROBIOTICS
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Funding OrganizationState Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/27977
Collection中科院水生所知识产出(2009年前)
Corresponding AuthorLi, Ai-Hua; Zhang, Yong-An
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Hubei, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
3.Huazhong Agr Univ, Coll Fisheries, State Key Lab Aquaculture Microbiol, Wuhan, Hubei, Peoples R China
4.Univ Chinese Acad Sci, Beijing, Peoples R China
5.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan, Hubei, Peoples R China
6.Freshwater Aquaculture Collaborat Innovat Ctr Hub, Wuhan, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Yao, Yuan-Yuan,Chen, Dan-Dan,Cui, Zheng-Wei,et al. Oral vaccination of tilapia against Streptococcus agalactiae using Bacillus subtilis spores expressing Sip[J]. FISH & SHELLFISH IMMUNOLOGY,2019,86:999-1008.
APA Yao, Yuan-Yuan.,Chen, Dan-Dan.,Cui, Zheng-Wei.,Zhang, Xiang-Yang.,Zhou, Yuan-Yuan.,...&Zhang, Yong-An.(2019).Oral vaccination of tilapia against Streptococcus agalactiae using Bacillus subtilis spores expressing Sip.FISH & SHELLFISH IMMUNOLOGY,86,999-1008.
MLA Yao, Yuan-Yuan,et al."Oral vaccination of tilapia against Streptococcus agalactiae using Bacillus subtilis spores expressing Sip".FISH & SHELLFISH IMMUNOLOGY 86(2019):999-1008.
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