IHB OpenIR  > 水生生物多样性与资源保护研究中心
DNA barcoding and evaluation of genetic diversity in Cyprinidae fish in the midstream of the Yangtze River
Shen, Yanjun1,2; Guan, Lihong3; Wang, Dengqiang4; Gan, Xiaoni1
2016-05-01
Source PublicationECOLOGY AND EVOLUTION
ISSN2045-7758
Volume6Issue:9Pages:2702-2713
AbstractThe Yangtze River is the longest river in China and is divided into upstream and mid-downstream regions by the Three Gorges (the natural barriers of the Yangtze River), resulting in a complex distribution of fish. Dramatic changes to habitat environments may ultimately threaten fish survival; thus, it is necessary to evaluate the genetic diversity and propose protective measures. Species identification is the most significant task in many fields of biological research and in conservation efforts. DNA barcoding, which constitutes the analysis of a short fragment of the mitochondrial cytochrome c oxidase subunit I (COI) sequence, has been widely used for species identification. In this study, we collected 561 COI barcode sequences from 35 fish from the midstream of the Yangtze River. The intraspecific distances of all species were below 2% (with the exception of Acheilognathus macropterus and Hemibarbus maculatus). Nevertheless, all species could be unambiguously identified from the trees, barcoding gaps and taxonomic resolution ratio values. Furthermore, the COI barcode diversity was found to be low (<= 0.5%), with the exception of H. maculatus (0.87%), A. macropterus (2.02%) and Saurogobio dabryi (0.82%). No or few shared haplotypes were detected between the upstream and downstream populations for ten species with overall nucleotide diversities greater than 0.00%, which indicated the likelihood of significant population genetic structuring. Our analyses indicated that DNA barcoding is an effective tool for the identification of cyprinidae fish in the midstream of the Yangtze River. It is vital that some protective measures be taken immediately because of the low COI barcode diversity.
SubtypeArticle
KeywordCyprinidae Cytochrome c Oxidase Subunit i Dna Barcoding Identification Kimura's Two-parameter
DOI10.1002/ece3.2060
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationKey Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture ; Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture ; Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture ; Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture
Language英语
WOS Research AreaEnvironmental Sciences & Ecology ; Evolutionary Biology
WOS SubjectEcology ; Evolutionary Biology
WOS KeywordFRESH-WATER FISHES ; BIOLOGICAL IDENTIFICATIONS ; MAXIMUM-LIKELIHOOD ; SUBSTITUTION ; SEAFOOD ; MARKET ; BRAZIL ; POPULATION ; TRADE ; CHINA
WOS IDWOS:000376149400006
Funding OrganizationKey Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture ; Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture ; Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture ; Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/27976
Collection水生生物多样性与资源保护研究中心
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
3.Xinxiang Med Univ, Coll Life Sci & Technol, Xinxiang 453003, Henan, Peoples R China
4.Chinese Acad Fishery Sci, Key Lab Freshwater Biodivers Conservat, Minist Agr, Yangtze River Fisheries Res Inst, Wuhan, Peoples R China
Recommended Citation
GB/T 7714
Shen, Yanjun,Guan, Lihong,Wang, Dengqiang,et al. DNA barcoding and evaluation of genetic diversity in Cyprinidae fish in the midstream of the Yangtze River[J]. ECOLOGY AND EVOLUTION,2016,6(9):2702-2713.
APA Shen, Yanjun,Guan, Lihong,Wang, Dengqiang,&Gan, Xiaoni.(2016).DNA barcoding and evaluation of genetic diversity in Cyprinidae fish in the midstream of the Yangtze River.ECOLOGY AND EVOLUTION,6(9),2702-2713.
MLA Shen, Yanjun,et al."DNA barcoding and evaluation of genetic diversity in Cyprinidae fish in the midstream of the Yangtze River".ECOLOGY AND EVOLUTION 6.9(2016):2702-2713.
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