Spring Viremia of Carp Virus N Protein Suppresses Fish IFN phi 1 Production by Targeting the Mitochondrial Antiviral Signaling Protein
Lu, Long-Feng1,2; Li, Shun1; Lu, Xiao-Bing1; LaPatra, Scott E.3; Zhang, Nu1,2; Zhang, Xu-Jie1,4; Chen, Dan-Dan1; Nie, Pin1; Zhang, Yong-An1
2016-05-01
Source PublicationJOURNAL OF IMMUNOLOGY
ISSN0022-1767
Volume196Issue:9Pages:3744-3753
AbstractFor a virus to replicate efficiently, it must try and inhibit host IFN expression because IFN is an important host defense at early stages after viral infection. For aquatic viruses, the mechanisms used to escape the hosts IFN system are still unclear. In this study, we show that the N protein of spring viremia of carp virus (SVCV) inhibits zebrafish IFN phi 1 production by degrading the mitochondrial antiviral signaling protein (MAVS). First, the upregulation of IFN phi 1 promoter activity stimulated by polyinosinic: polycytidylic acid, retinoic acid-inducible gene I (RIG-I) or MAVS was suppressed by the SVCV infection. However, the upregulation by the downstream factor of the RIG-I-like receptor signaling pathway, TANK-binding kinase 1, was not affected. Notably, at the protein level, MAVS decreased remarkably when cells were infected with SVCV. Second, consistent with the result of the SVCV infection, overexpression of the N protein of SVCV blocked the IFN phi 1 transcription activated by MAVS and downregulated MAVS expression at the protein level but not at the mRNA level. Further analysis demonstrated that the N protein targeted MAVS for K48-linked ubiquitination, which promoted the degradation of MAVS. These data indicated that fish MAVS could be degraded by the N protein of SVCV through the ubiquitin-proteasome pathway. To our knowledge, this is the first article of a fish RIG-I-like receptor pathway interfered by an aquatic virus in an ubiquitin-proteasome manner, suggesting that immune evasion of a virus also exists in lower vertebrates.
SubtypeArticle
DOI10.4049/jimmunol.1502038
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNational Key Basic Research Program of China(2014CB138601) ; National Key Basic Research Program of China(2014CB138601) ; National Natural Science Foundation of China(31172431) ; National Natural Science Foundation of China(31172431) ; National Key Basic Research Program of China(2014CB138601) ; National Key Basic Research Program of China(2014CB138601) ; National Natural Science Foundation of China(31172431) ; National Natural Science Foundation of China(31172431)
Language英语
WOS Research AreaImmunology
WOS SubjectImmunology
WOS KeywordINFLUENZA-A VIRUS ; DOUBLE-STRANDED-RNA ; INTERFERON REGULATORY FACTOR-3 ; PAPULOSUM CYPRINI CELLS ; INNATE IMMUNE-RESPONSE ; HEPATITIS-C-VIRUS ; NS1 PROTEIN ; KAPPA-B ; RIG-I ; PATHOGEN RECOGNITION
WOS IDWOS:000374450900027
Funding OrganizationNational Key Basic Research Program of China(2014CB138601) ; National Key Basic Research Program of China(2014CB138601) ; National Natural Science Foundation of China(31172431) ; National Natural Science Foundation of China(31172431) ; National Key Basic Research Program of China(2014CB138601) ; National Key Basic Research Program of China(2014CB138601) ; National Natural Science Foundation of China(31172431) ; National Natural Science Foundation of China(31172431)
Citation statistics
Cited Times:22[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/27973
Collection藻类生物学及应用研究中心_水生生物分子与细胞生物学研究中心
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, 7 Donghu South Rd, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Clear Spring Foods Inc, Div Res, Buhl, ID 83316 USA
4.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
Recommended Citation
GB/T 7714
Lu, Long-Feng,Li, Shun,Lu, Xiao-Bing,et al. Spring Viremia of Carp Virus N Protein Suppresses Fish IFN phi 1 Production by Targeting the Mitochondrial Antiviral Signaling Protein[J]. JOURNAL OF IMMUNOLOGY,2016,196(9):3744-3753.
APA Lu, Long-Feng.,Li, Shun.,Lu, Xiao-Bing.,LaPatra, Scott E..,Zhang, Nu.,...&Zhang, Yong-An.(2016).Spring Viremia of Carp Virus N Protein Suppresses Fish IFN phi 1 Production by Targeting the Mitochondrial Antiviral Signaling Protein.JOURNAL OF IMMUNOLOGY,196(9),3744-3753.
MLA Lu, Long-Feng,et al."Spring Viremia of Carp Virus N Protein Suppresses Fish IFN phi 1 Production by Targeting the Mitochondrial Antiviral Signaling Protein".JOURNAL OF IMMUNOLOGY 196.9(2016):3744-3753.
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