| Dietary choline regulates antibacterial activity, inflammatory response and barrier function in the gills of grass carp (Ctenopharyngodon idella) | |
| Zhao, Hua-Fu1; Jiang, Wei-Dan1,2,3; Liu, Yang1,2,3; Jiang, Jun1,2,3; Wu, Pei1,2,3; Kuang, Sheng-Yao4; Tang, Ling4; Tang, Wu-Neng4; Zhang, Yong-An5; Zhou, Xiao-Qiu1,2,3; Feng, Lin1,2,3 | |
| 2016-05-01 | |
| Source Publication | FISH & SHELLFISH IMMUNOLOGY
 |
| ISSN | 1050-4648 |
| Volume | 52Issue:*Pages:139-150 |
| Abstract | An 8-week feeding trial was conducted to determine the effects of graded levels of choline (197-1795 mg/kg) on antibacterial properties, inflammatory status and barrier function in the gills of grass carp. The results showed that optimal dietary choline supplementation significantly improved lysozyme and acid phosphatase activities, complement component 3 (C3) content, and the liver expressed antimicrobial peptide 2 and Hepcidin mRNA levels in the gills of fish (P < 0.05). In addition, appropriate dietary choline significantly decreased the oxidative damage, which might be partly due to increase copper, zinc superoxide dismutase (Cu/Zn-SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and glutathione reductase (GR) activities and increased glutathione content in the gills of fish (P < 0.05). Moreover, appropriate dietary choline significantly up-regulated the mRNA levels of interleukin 10 and transforming growth factor 01, Zonula occludens 1, Occludin, Claudin-b, c, 3 and 12, inhibitor of kappa B alpha, target of rapamycin, Cu/Zn-SOD, CAT, GR, GPx, GST and NF-E2-related factor 2 in the gills of fish (P < 0.05). Conversely, appropriate dietary choline significantly down-regulated the mRNA levels of pro-inflammatory cytokines, tumor necrosis factor alpha, interleukin 8, interferon gamma, interleukin 1 beta, and related signaling factors, nuclear factor kappa B p65, I kappa B kinase beta, I kappa B kinase gamma, myosin light chain kinase and Kelch-like-ECH-associated protein 1a (Keap1a) in the gills of fish (P < 0.05). However, choline did not have a significant effect on the mRNA levels of I kappa B kinase alpha, Claudin-15 and Keap1b in the gills of fish. Collectively, appropriate dietary choline levels improved gill antibacterial properties and relative gene expression levels of tight junction proteins, and decreased inflammatory status, as well as up-regulated the mRNA levels of related signaling molecules in the gills of fish. Based on gill C3 content and AHR activity, the dietary choline requirements for young grass carp (266.5-787.1 g) were estimated to be 1191.0 and 1555.0 mg/kg diet, respectively. (C) 2016 Published by Elsevier Ltd. |
| Subtype | Article |
| Keyword | Choline Grass Carp Tight Junction Protein Gill Antioxidant Nrf2 |
| DOI | 10.1016/j.fsi.2016.03.029 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Indexed By | SCI |
| Funding Organization | National Basic Research Program of China (973 Program)(2014CB138600) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; 2013NC0045) ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; 2013NC0045) |
| Language | 英语 |
| WOS Research Area | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS Subject | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS Keyword | NF-KAPPA-B ; TIGHT JUNCTION PROTEINS ; TROUT ONCORHYNCHUS-MYKISS ; MESSENGER-RNA EXPRESSION ; MUCOSAL IMMUNE-SYSTEM ; ANTIOXIDANT STATUS ; SIGNALING PATHWAY ; OXIDATIVE STRESS ; JUVENILE JIAN ; TNF-ALPHA |
| WOS ID | WOS:000375165200014 |
| Funding Organization | National Basic Research Program of China (973 Program)(2014CB138600) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; 2013NC0045) ; National Basic Research Program of China (973 Program)(2014CB138600) ; National Department Public Benefit Research Foundation (Agriculture) of China(201003020) ; Science and Technology Support Program of Sichuan Province of China(2014NZ0003) ; Specialized Research Fund for the Doctoral Program of Higher Education of China(20135103110001) ; Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2012NC0007 ; Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China(2015CC0011) ; Natural Science Foundation for Young Scientists of Sichuan Province(2014JQ0007) ; Sichuan Province Research Foundation for Basic Research(2013JY0082) ; 2013NC0045) |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/27962 |
| Collection | 藻类生物学及应用研究中心_水生生物分子与细胞生物学研究中心 |
| Affiliation | 1.Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Peoples R China 2.Sichuan Agr Univ, Fish Nutr & Safety Prod Univ, Key Lab Sichuan Prov, Chengdu 611130, Peoples R China 3.Sichuan Agr Univ, China Minist Educ, Key Lab Anim Dis Resistance Nutr, Chengdu 611130, Peoples R China 4.Sichuan Acad Anim Sci, Inst Anim Nutr, Chengdu 610066, Peoples R China 5.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| Recommended Citation GB/T 7714 | Zhao, Hua-Fu,Jiang, Wei-Dan,Liu, Yang,et al. Dietary choline regulates antibacterial activity, inflammatory response and barrier function in the gills of grass carp (Ctenopharyngodon idella)[J]. FISH & SHELLFISH IMMUNOLOGY,2016,52(*):139-150. |
| APA | Zhao, Hua-Fu.,Jiang, Wei-Dan.,Liu, Yang.,Jiang, Jun.,Wu, Pei.,...&Feng, Lin.(2016).Dietary choline regulates antibacterial activity, inflammatory response and barrier function in the gills of grass carp (Ctenopharyngodon idella).FISH & SHELLFISH IMMUNOLOGY,52(*),139-150. |
| MLA | Zhao, Hua-Fu,et al."Dietary choline regulates antibacterial activity, inflammatory response and barrier function in the gills of grass carp (Ctenopharyngodon idella)".FISH & SHELLFISH IMMUNOLOGY 52.*(2016):139-150. |
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