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题名: Identification and characterization of Bacillus subtilis from grass carp (Ctenopharynodon idellus) for use as probiotic additives in aquatic feed
作者: Guo, Xia1, 2; Chen, Dan-Dan1; Peng, Kai-Song1, 3; Cui, Zheng-Wei1, 4; Zhang, Xu-Jie1, 5; Li, Shun1; Zhang, Yong-An1
关键词: Bacillus subtilis ; Probiotic ; Stress condition resistance ; Anti-inflammation ; Grass carp
刊名: FISH & SHELLFISH IMMUNOLOGY
发表日期: 2016-05-01
DOI: 10.1016/j.fsi.2016.03.017
卷: 52, 期:*, 页:74-84
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
英文摘要: Bacillus subtilis is widely used as probiotic species in aquaculture for water quality control, growth promoting, or immunity enhancing. The aim of this study is to find novel B. subtilis strains from fish as potential probiotics for aquaculture. Eleven B. subtilis isolates derived from the intestinal tract of grass carp were identified by gene sequencing and biochemical tests. These isolates were classified into 4 groups, and the representatives (GC-5, GC-6, GC-21 and GC-22) of each group were further investigated for antibiotic susceptibility, sporulation rate, biofilm formation, activity against pathogenic bacteria, resistance to stress conditions of intestinal tract (high percentage of bile and low pH) and high temperature, which are important for probiotics to be used as feed additives. Additionally, the adhesion properties of the 4 characterized strains were assessed using Caco-2 cell and gut mucus models. The results showed that the 4 strains differed in their capacities to adhere to intestinal epithelial cells and mucus. Furthermore, the strains GC-21 and GC-22 up-regulated the expression levels of IL-10 and TGF-beta but down-regulated IL-1 beta, suggesting their potential anti-inflammatory abilities. Based on physiological properties of the 4 characterized B. subtilis strains, one or more strains may have potential to be used as probiotics in aquaculture. (C) 2016 Elsevier Ltd. All rights reserved.
关键词[WOS]: LACTIC-ACID BACTERIA ; SPARUS-AURATA L. ; GASTROINTESTINAL-TRACT ; BIOFILM FORMATION ; IN-VITRO ; OREOCHROMIS-NILOTICUS ; DISEASE RESISTANCE ; IMMUNE PARAMETERS ; GENE-EXPRESSION ; RAINBOW-TROUT
语种: 英语
项目资助者: National Basic Research Program of China(2014CB138601) ; 3551 Optics Valley Talent Program
WOS记录号: WOS:000375165200009
ISSN号: 1050-4648
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/27958
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Peoples R China
3.Anhui Agr Univ, Hefei 230036, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
5.Shanghai Ocean Univ, Shanghai 201306, Peoples R China

Recommended Citation:
Guo, Xia,Chen, Dan-Dan,Peng, Kai-Song,et al. Identification and characterization of Bacillus subtilis from grass carp (Ctenopharynodon idellus) for use as probiotic additives in aquatic feed[J]. FISH & SHELLFISH IMMUNOLOGY,2016,52(*):74-84.
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