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Microcystin-degrading bacteria affect mcyD expression and microcystin synthesis in Microcystis spp.
Zhu, Lin1; Zuo, Jun1,2; Song, Lirong1; Gan, Nanqin1
2016-03-01
Source PublicationJOURNAL OF ENVIRONMENTAL SCIENCES
ISSN1001-0742
Volume41Pages:195-201
AbstractCyanobacterial blooms occur increasingly often and cause ecological, economic and human health problems worldwide. Microcystins (MCs) are the dominant toxins produced by cyanobacteria and are implicated in epidemic disease and environmental problems. Extensive research has been reported on the various regulating factors, e. g., light, temperature, nutrients such as nitrogen and phosphorus, pH, iron, xenobiotics, and predators, that influence microcystin (MC) synthesis, but little is known about the effects of cyanobacteria-associated bacteria on MC synthesis. A considerable number of studies have focused on interactions between Microcystis species and their associated bacteria. In this study, we evaluated the effects of MC-degrading bacteria (MCDB) on MC synthesis gene mcyD expression and MC synthesis in axenic strain PCC7806, non-axenic strain FACHB905, and colony strain FACHB1325 of Microcystis by quantitative real-time polymerase chain reaction (RT-PCR) assay and enzyme-linked immunosorbent assay (ELISA). We demonstrate for the first time that MCDB can induce and up-regulate the MC production and transcriptional response of the mcyD gene of toxic Microcystis. On day 4 of the culturing experiment, the intracellular MC concentration and transcriptional response of mcyD of FACHB1325 were up-regulated 1.9 and 5.3-fold over that of the control, and for FACHB905 were up-regulated 1.8 and 4.2-fold over that of the control, respectively. On day 10, the transcriptional response of mcyD was up-regulated 21.3-fold in PCC7806. These results indicate that there are interactions between toxic Microcystis and MCDB, and MCDB may play a role in regulating mcyD expression in toxic Microcystis. (C) 2015 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V.
SubtypeArticle
KeywordMicrocystis Microcystins Mc-degrading Bacteria Mcyd Gene
DOI10.1016/j.jes.2015.06.016
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationChinese Academy of Sciences(KSCX2-EW-Z-3) ; Chinese Academy of Sciences(KSCX2-EW-Z-3) ; National Natural Science Foundation of China(31370418 ; National Natural Science Foundation of China(31370418 ; State Key Laboratory of Freshwater Ecology and Biotechnology(2011FBZ16) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2011FBZ16) ; 31123001) ; 31123001) ; Chinese Academy of Sciences(KSCX2-EW-Z-3) ; Chinese Academy of Sciences(KSCX2-EW-Z-3) ; National Natural Science Foundation of China(31370418 ; National Natural Science Foundation of China(31370418 ; State Key Laboratory of Freshwater Ecology and Biotechnology(2011FBZ16) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2011FBZ16) ; 31123001) ; 31123001)
Language英语
WOS Research AreaEnvironmental Sciences & Ecology
WOS SubjectEnvironmental Sciences
WOS KeywordBLOOM-FORMING MICROCYSTIS ; GENE-EXPRESSION ; AERUGINOSA CYANOBACTERIA ; ENZYMATIC PATHWAY ; LR ; DEGRADATION ; WATER ; LAKE ; GROWTH ; ALGAE
WOS IDWOS:000373231400022
Funding OrganizationChinese Academy of Sciences(KSCX2-EW-Z-3) ; Chinese Academy of Sciences(KSCX2-EW-Z-3) ; National Natural Science Foundation of China(31370418 ; National Natural Science Foundation of China(31370418 ; State Key Laboratory of Freshwater Ecology and Biotechnology(2011FBZ16) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2011FBZ16) ; 31123001) ; 31123001) ; Chinese Academy of Sciences(KSCX2-EW-Z-3) ; Chinese Academy of Sciences(KSCX2-EW-Z-3) ; National Natural Science Foundation of China(31370418 ; National Natural Science Foundation of China(31370418 ; State Key Laboratory of Freshwater Ecology and Biotechnology(2011FBZ16) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2011FBZ16) ; 31123001) ; 31123001)
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Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/27943
Collection藻类生物学及应用研究中心
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Zhu, Lin,Zuo, Jun,Song, Lirong,et al. Microcystin-degrading bacteria affect mcyD expression and microcystin synthesis in Microcystis spp.[J]. JOURNAL OF ENVIRONMENTAL SCIENCES,2016,41:195-201.
APA Zhu, Lin,Zuo, Jun,Song, Lirong,&Gan, Nanqin.(2016).Microcystin-degrading bacteria affect mcyD expression and microcystin synthesis in Microcystis spp..JOURNAL OF ENVIRONMENTAL SCIENCES,41,195-201.
MLA Zhu, Lin,et al."Microcystin-degrading bacteria affect mcyD expression and microcystin synthesis in Microcystis spp.".JOURNAL OF ENVIRONMENTAL SCIENCES 41(2016):195-201.
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