IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心
Two hepcidins from spotted scat (Scatophagus argus) possess antibacterial and antiviral functions in vitro
Gui, Lang1; Zhang, Peipei1; Zhang, Qiya2; Zhang, Junbin1
2016-03-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume50Pages:191-199
AbstractHepcidins are small cysteine-rich antimicrobial peptides that play an important role in host immunity against pathogenic organisms. In this study, two hepcidins, SA-hepcidin1 and SA-hepcidin2, were cloned from spotted scat (Scatophagus argus), and the tissue distributions of SA-hepcidins were determined. In addition, mature SA-hepcidin peptides were synthesized to allow evaluation of their antimicrobial and antiviral functions in vitro. SA-hepcidinl belongs to the HAMP1 class and is widely expressed in all tested tissues from spotted scat, whereas SA-hepcidin2 belongs to the HAMP2 class and present only in the liver. The synthetic SA-hepcidins had similar levels of antibacterial activity against Gram-positive and Gram-negative bacteria; however, the antibacterial activity of SA-hepcidinl was stronger than that of SA-hepcidin2. The antiviral activities of the synthetic SA-hepcidins were assessed against Siniperca chuatsi rhabdovirus (SCRV) and largemouth bass Micropterus salmoides reovirus (MsReV) in epithelioma papulosum cyprini (EPC) and grass carp fin (GCF) cells. SA-hepcidin2 had antiviral activity, but SA-hepcidinl did not. The results of this study suggest that SA-hepcidins are important multifunctional proteins in the spotted scat immune system that are involved in resistance to various pathogens. (C) 2016 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordHepcidin Scatophagus Argus Antibacterial Activity Antiviral Activity Amps Synthetic Peptide Scrv Msrev Epc Gcf
DOI10.1016/j.fsi.2016.01.038
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNational Natural Science Foundation of China(31272662 ; National Natural Science Foundation of China(31272662 ; National Key Technology R&D Program from the Ministry of Science and Technology, Shanghai University First-class Disciplines Project of Fisheries(2011BAD13B01) ; National Key Technology R&D Program from the Ministry of Science and Technology, Shanghai University First-class Disciplines Project of Fisheries(2011BAD13B01) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2014FB02) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2014FB02) ; Shanghai Ocean University Major Project Science Foundation of Graduate Students(A-0209-13-0105392) ; Shanghai Ocean University Major Project Science Foundation of Graduate Students(A-0209-13-0105392) ; 31302214) ; 31302214) ; National Natural Science Foundation of China(31272662 ; National Natural Science Foundation of China(31272662 ; National Key Technology R&D Program from the Ministry of Science and Technology, Shanghai University First-class Disciplines Project of Fisheries(2011BAD13B01) ; National Key Technology R&D Program from the Ministry of Science and Technology, Shanghai University First-class Disciplines Project of Fisheries(2011BAD13B01) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2014FB02) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2014FB02) ; Shanghai Ocean University Major Project Science Foundation of Graduate Students(A-0209-13-0105392) ; Shanghai Ocean University Major Project Science Foundation of Graduate Students(A-0209-13-0105392) ; 31302214) ; 31302214)
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS KeywordTURBOT SCOPHTHALMUS-MAXIMUS ; ANTIMICROBIAL PEPTIDE ; PARALICHTHYS-OLIVACEUS ; MOLECULAR-CLONING ; JAPANESE FLOUNDER ; GENE-EXPRESSION ; MANDARIN FISH ; TILAPIA ; INFECTION ; BACTERIAL
WOS IDWOS:000372693800022
Funding OrganizationNational Natural Science Foundation of China(31272662 ; National Natural Science Foundation of China(31272662 ; National Key Technology R&D Program from the Ministry of Science and Technology, Shanghai University First-class Disciplines Project of Fisheries(2011BAD13B01) ; National Key Technology R&D Program from the Ministry of Science and Technology, Shanghai University First-class Disciplines Project of Fisheries(2011BAD13B01) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2014FB02) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2014FB02) ; Shanghai Ocean University Major Project Science Foundation of Graduate Students(A-0209-13-0105392) ; Shanghai Ocean University Major Project Science Foundation of Graduate Students(A-0209-13-0105392) ; 31302214) ; 31302214) ; National Natural Science Foundation of China(31272662 ; National Natural Science Foundation of China(31272662 ; National Key Technology R&D Program from the Ministry of Science and Technology, Shanghai University First-class Disciplines Project of Fisheries(2011BAD13B01) ; National Key Technology R&D Program from the Ministry of Science and Technology, Shanghai University First-class Disciplines Project of Fisheries(2011BAD13B01) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2014FB02) ; State Key Laboratory of Freshwater Ecology and Biotechnology(2014FB02) ; Shanghai Ocean University Major Project Science Foundation of Graduate Students(A-0209-13-0105392) ; Shanghai Ocean University Major Project Science Foundation of Graduate Students(A-0209-13-0105392) ; 31302214) ; 31302214)
Citation statistics
Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/27926
Collection鱼类生物学及渔业生物技术研究中心
Affiliation1.Shanghai Ocean Univ, Key Lab Explorat & Utilizat Aquat Genet Resources, Minist Educ, Shanghai 201306, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Gui, Lang,Zhang, Peipei,Zhang, Qiya,et al. Two hepcidins from spotted scat (Scatophagus argus) possess antibacterial and antiviral functions in vitro[J]. FISH & SHELLFISH IMMUNOLOGY,2016,50:191-199.
APA Gui, Lang,Zhang, Peipei,Zhang, Qiya,&Zhang, Junbin.(2016).Two hepcidins from spotted scat (Scatophagus argus) possess antibacterial and antiviral functions in vitro.FISH & SHELLFISH IMMUNOLOGY,50,191-199.
MLA Gui, Lang,et al."Two hepcidins from spotted scat (Scatophagus argus) possess antibacterial and antiviral functions in vitro".FISH & SHELLFISH IMMUNOLOGY 50(2016):191-199.
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