IHB OpenIR  > 其他
NS38 is required for aquareovirus replication via interaction with viral core proteins and host eIF3A
Zhang, Jie1,2; Guo, Hong1; Zhang, Fuxian1; Chen, Qingxiu1; Chang, Mingxian2; Fang, Qin1
Corresponding AuthorChang, Mingxian(mingxianchang@ihb.ac.cn) ; Fang, Qin(qfang@wh.iov.cn)
2019-03-01
Source PublicationVIROLOGY
ISSN0042-6822
Volume529Pages:216-225
AbstractAquareoviruses contain an 11-segmented double-stranded RNA genome. Previous studies indicated that NS38, a virus-encoded putative single-stranded RNA binding protein, interacts with NS80 in viral inclusion bodies (VIBs). However, the role of NS38 in aquareovirus infection remained unclear. Here, we found that NS38 interacts with inner-capsid proteins (VP1 VP4 and VP6) and the NS80-RNA complex in both transfected and infected cells. Knockdown of NS38 by siRNAs-115/219 clearly reduced viral infection, with decreased mRNA and protein yields. Moreover, NS38 can interact with host cellular eukaryotic translation initiation factor 3 subunit A (eIF3A) in transfected cells, while no association was detected between eIF3A and NS80. This study is the first to define that the NS38 is essential to viral replication. Together, our findings indicate that NS38 might function as a mediator by interacting with viral and host cellular components in VIB5 during replication.
KeywordAquareovirus NS38 Virus host cell interaction Replication
DOI10.1016/j.virol.2019.01.029
Funding OrganizationNational Natural Science Foundation of China and Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province ; National Natural Science Foundation of China and Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province ; National Natural Science Foundation of China and Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province ; National Natural Science Foundation of China and Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province ; National Natural Science Foundation of China and Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province ; National Natural Science Foundation of China and Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province ; National Natural Science Foundation of China and Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province ; National Natural Science Foundation of China and Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province ; National Natural Science Foundation of China and Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province ; National Natural Science Foundation of China and Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province ; National Natural Science Foundation of China and Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province ; National Natural Science Foundation of China and Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province ; National Natural Science Foundation of China and Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province ; National Natural Science Foundation of China and Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province ; National Natural Science Foundation of China and Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province ; National Natural Science Foundation of China and Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province
Indexed BySCI ; SCI
Language英语
Funding ProjectNational Natural Science Foundation of China and Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province[31672693] ; National Natural Science Foundation of China and Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province[2018CFA011] ; National Natural Science Foundation of China and Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province[31372565] ; National Natural Science Foundation of China and Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province[31402340]
WOS Research AreaVirology
WOS SubjectVirology
WOS IDWOS:000460195900024
WOS KeywordGRASS CARP REOVIRUS ; VIRUS ; IDENTIFICATION ; ROTAVIRUS ; AGGRESOMES ; SITES ; RNA ; ASSOCIATION ; TRANSLATION ; EXPRESSION
PublisherACADEMIC PRESS INC ELSEVIER SCIENCE
Funding OrganizationNational Natural Science Foundation of China and Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province ; National Natural Science Foundation of China and Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province ; National Natural Science Foundation of China and Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province ; National Natural Science Foundation of China and Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province ; National Natural Science Foundation of China and Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province ; National Natural Science Foundation of China and Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province ; National Natural Science Foundation of China and Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province ; National Natural Science Foundation of China and Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province ; National Natural Science Foundation of China and Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province ; National Natural Science Foundation of China and Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province ; National Natural Science Foundation of China and Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province ; National Natural Science Foundation of China and Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province ; National Natural Science Foundation of China and Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province ; National Natural Science Foundation of China and Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province ; National Natural Science Foundation of China and Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province ; National Natural Science Foundation of China and Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/27910
Collection其他
Corresponding AuthorChang, Mingxian; Fang, Qin
Affiliation1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China
2.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Jie,Guo, Hong,Zhang, Fuxian,et al. NS38 is required for aquareovirus replication via interaction with viral core proteins and host eIF3A[J]. VIROLOGY,2019,529:216-225.
APA Zhang, Jie,Guo, Hong,Zhang, Fuxian,Chen, Qingxiu,Chang, Mingxian,&Fang, Qin.(2019).NS38 is required for aquareovirus replication via interaction with viral core proteins and host eIF3A.VIROLOGY,529,216-225.
MLA Zhang, Jie,et al."NS38 is required for aquareovirus replication via interaction with viral core proteins and host eIF3A".VIROLOGY 529(2019):216-225.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhang, Jie]'s Articles
[Guo, Hong]'s Articles
[Zhang, Fuxian]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhang, Jie]'s Articles
[Guo, Hong]'s Articles
[Zhang, Fuxian]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhang, Jie]'s Articles
[Guo, Hong]'s Articles
[Zhang, Fuxian]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.