| Biosynthesis of Triacylglycerol Molecules with a Tailored PUFA Profile in Industrial Microalgae | |
| Xin, Yi1,2,5,6; Shen, Chen1,2,5,6; She, Yiting1,2,5,6; Chen, Hong3; Wang, Cong4; Wei, Li1,2,5,6; Yoon, Kangsup3; Han, Danxiang3; Hu, Qiang3,5; Xu, Jian1,2,5,6 | |
| Corresponding Author | Xu, Jian(xujian@qibebt.ac.cn) |
| 2019-04-01 | |
| Source Publication | MOLECULAR PLANT
 |
| ISSN | 1674-2052 |
| Volume | 12Issue:4Pages:474-488 |
| Abstract | The composition of polyunsaturated fatty acids (PUFAs) in triacylglycerols (TAGs) is key to health benefits and for oil applications, yet the underlying genetic mechanism remains poorly understood. In this study, by in silico, ex vivo, and in vivo profiling of type-2 diacylglycerol acyltransferases (DGAT2s) in Nannochloropsis oceanica we revealed two novel PUFA-preferring enzymes that discriminate individual PUFA species in TAG assembly, with NoDGAT2J for linoleic acid (LA) and NoDGAT2K for eicosapentaenoic acid (EPA). The LA and EPA composition of TAG molecules is mediated in vivo via the functional partitioning between NoDGAT2J and 2K, both of which are localized in the chloroplast envelope. By modulating transcript abundance of the DGAT2s, an N. oceanica strain bank was created, where proportions of LA and EPA in TAG vary by 18.7-fold (between 0.21% and 3.92% dry weight) and 34.7-fold (between 0.09% and 3.12% dry weight), respectively. These findings lay the foundation for producing designer TAG molecules with tailored health benefits or for biofuel applications in industrial microalgae and higher-plant crops. |
| Keyword | microalgae polyunsaturated fatty acids diacylglycerol acyltransferase Nannochloropsis oceanica |
| DOI | 10.1016/j.molp.2018.12.007 |
| Funding Organization | Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of Shandong, China ; Natural Science Foundation of Shandong, China ; Natural Science Foundation of Shandong, China ; Natural Science Foundation of Shandong, China ; Biological Carbon Sequestration Program from the Chinese Academy of Sciences ; Biological Carbon Sequestration Program from the Chinese Academy of Sciences ; Biological Carbon Sequestration Program from the Chinese Academy of Sciences ; Biological Carbon Sequestration Program from the Chinese Academy of Sciences ; Chinese Key R&D Plan for Synthetic Biology Project ; Chinese Key R&D Plan for Synthetic Biology Project ; Chinese Key R&D Plan for Synthetic Biology Project ; Chinese Key R&D Plan for Synthetic Biology Project ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of Shandong, China ; Natural Science Foundation of Shandong, China ; Natural Science Foundation of Shandong, China ; Natural Science Foundation of Shandong, China ; Biological Carbon Sequestration Program from the Chinese Academy of Sciences ; Biological Carbon Sequestration Program from the Chinese Academy of Sciences ; Biological Carbon Sequestration Program from the Chinese Academy of Sciences ; Biological Carbon Sequestration Program from the Chinese Academy of Sciences ; Chinese Key R&D Plan for Synthetic Biology Project ; Chinese Key R&D Plan for Synthetic Biology Project ; Chinese Key R&D Plan for Synthetic Biology Project ; Chinese Key R&D Plan for Synthetic Biology Project ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of Shandong, China ; Natural Science Foundation of Shandong, China ; Natural Science Foundation of Shandong, China ; Natural Science Foundation of Shandong, China ; Biological Carbon Sequestration Program from the Chinese Academy of Sciences ; Biological Carbon Sequestration Program from the Chinese Academy of Sciences ; Biological Carbon Sequestration Program from the Chinese Academy of Sciences ; Biological Carbon Sequestration Program from the Chinese Academy of Sciences ; Chinese Key R&D Plan for Synthetic Biology Project ; Chinese Key R&D Plan for Synthetic Biology Project ; Chinese Key R&D Plan for Synthetic Biology Project ; Chinese Key R&D Plan for Synthetic Biology Project ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of Shandong, China ; Natural Science Foundation of Shandong, China ; Natural Science Foundation of Shandong, China ; Natural Science Foundation of Shandong, China ; Biological Carbon Sequestration Program from the Chinese Academy of Sciences ; Biological Carbon Sequestration Program from the Chinese Academy of Sciences ; Biological Carbon Sequestration Program from the Chinese Academy of Sciences ; Biological Carbon Sequestration Program from the Chinese Academy of Sciences ; Chinese Key R&D Plan for Synthetic Biology Project ; Chinese Key R&D Plan for Synthetic Biology Project ; Chinese Key R&D Plan for Synthetic Biology Project ; Chinese Key R&D Plan for Synthetic Biology Project |
| Indexed By | SCI ; SCI |
| Language | 英语 |
| Funding Project | Natural Science Foundation of China[31600059] ; Natural Science Foundation of China[31425002] ; Natural Science Foundation of China[31741005] ; Natural Science Foundation of Shandong, China[ZR2015CQ003] ; Natural Science Foundation of Shandong, China[ZR201709180185] ; Biological Carbon Sequestration Program from the Chinese Academy of Sciences[ZDRW-ZS-2016-3] ; Chinese Key R&D Plan for Synthetic Biology Project |
| WOS Research Area | Biochemistry & Molecular Biology ; Plant Sciences |
| WOS Subject | Biochemistry & Molecular Biology ; Plant Sciences |
| WOS ID | WOS:000462878700006 |
| WOS Keyword | FATTY-ACID-COMPOSITION ; DIACYLGLYCEROL ACYLTRANSFERASE ; NANNOCHLOROPSIS-OCEANICA ; OLEAGINOUS MICROALGA ; NITROGEN-DEPLETION ; STRESS ; REVEALS ; LIPIDS ; LOCALIZATION ; METABOLISM |
| Publisher | CELL PRESS |
| Funding Organization | Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of Shandong, China ; Natural Science Foundation of Shandong, China ; Natural Science Foundation of Shandong, China ; Natural Science Foundation of Shandong, China ; Biological Carbon Sequestration Program from the Chinese Academy of Sciences ; Biological Carbon Sequestration Program from the Chinese Academy of Sciences ; Biological Carbon Sequestration Program from the Chinese Academy of Sciences ; Biological Carbon Sequestration Program from the Chinese Academy of Sciences ; Chinese Key R&D Plan for Synthetic Biology Project ; Chinese Key R&D Plan for Synthetic Biology Project ; Chinese Key R&D Plan for Synthetic Biology Project ; Chinese Key R&D Plan for Synthetic Biology Project ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of Shandong, China ; Natural Science Foundation of Shandong, China ; Natural Science Foundation of Shandong, China ; Natural Science Foundation of Shandong, China ; Biological Carbon Sequestration Program from the Chinese Academy of Sciences ; Biological Carbon Sequestration Program from the Chinese Academy of Sciences ; Biological Carbon Sequestration Program from the Chinese Academy of Sciences ; Biological Carbon Sequestration Program from the Chinese Academy of Sciences ; Chinese Key R&D Plan for Synthetic Biology Project ; Chinese Key R&D Plan for Synthetic Biology Project ; Chinese Key R&D Plan for Synthetic Biology Project ; Chinese Key R&D Plan for Synthetic Biology Project ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of Shandong, China ; Natural Science Foundation of Shandong, China ; Natural Science Foundation of Shandong, China ; Natural Science Foundation of Shandong, China ; Biological Carbon Sequestration Program from the Chinese Academy of Sciences ; Biological Carbon Sequestration Program from the Chinese Academy of Sciences ; Biological Carbon Sequestration Program from the Chinese Academy of Sciences ; Biological Carbon Sequestration Program from the Chinese Academy of Sciences ; Chinese Key R&D Plan for Synthetic Biology Project ; Chinese Key R&D Plan for Synthetic Biology Project ; Chinese Key R&D Plan for Synthetic Biology Project ; Chinese Key R&D Plan for Synthetic Biology Project ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of Shandong, China ; Natural Science Foundation of Shandong, China ; Natural Science Foundation of Shandong, China ; Natural Science Foundation of Shandong, China ; Biological Carbon Sequestration Program from the Chinese Academy of Sciences ; Biological Carbon Sequestration Program from the Chinese Academy of Sciences ; Biological Carbon Sequestration Program from the Chinese Academy of Sciences ; Biological Carbon Sequestration Program from the Chinese Academy of Sciences ; Chinese Key R&D Plan for Synthetic Biology Project ; Chinese Key R&D Plan for Synthetic Biology Project ; Chinese Key R&D Plan for Synthetic Biology Project ; Chinese Key R&D Plan for Synthetic Biology Project |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/27856 |
| Collection | 藻类生物技术和生物能源研发中心 |
| Corresponding Author | Xu, Jian |
| Affiliation | 1.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, CAS Key Lab Biofuels, Single Cell Ctr, Qingdao 266101, Shandong, Peoples R China 2.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Shandong Lab Energy Genet, Qingdao 266101, Shandong, Peoples R China 3.Chinese Acad Sci, Ctr Microalgal Biotechnol & Biofuels, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China 4.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Core Lab, Shandong 266101, Shandong, Peoples R China 5.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Shandong 266237, Shandong, Peoples R China 6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| Recommended Citation GB/T 7714 | Xin, Yi,Shen, Chen,She, Yiting,et al. Biosynthesis of Triacylglycerol Molecules with a Tailored PUFA Profile in Industrial Microalgae[J]. MOLECULAR PLANT,2019,12(4):474-488. |
| APA | Xin, Yi.,Shen, Chen.,She, Yiting.,Chen, Hong.,Wang, Cong.,...&Xu, Jian.(2019).Biosynthesis of Triacylglycerol Molecules with a Tailored PUFA Profile in Industrial Microalgae.MOLECULAR PLANT,12(4),474-488. |
| MLA | Xin, Yi,et al."Biosynthesis of Triacylglycerol Molecules with a Tailored PUFA Profile in Industrial Microalgae".MOLECULAR PLANT 12.4(2019):474-488. |
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