| Abundance of Early Embryonic Primordial Germ Cells Promotes Zebrafish Female Differentiation as Revealedby Lifetime Labeling of Germline | |
| Ye, Ding1,2; Zhu, Lin1,2; Zhang, Qifeng1,2; Xiong, Feng1; Wang, Houpeng1; Wang, Xiaosi1,2; He, Mudan1,2; Zhu, Zuoyan1,2; Sun, Yonghua1,2 | |
| Corresponding Author | Sun, Yonghua(yhsun@ihb.ac.cn) |
| 2019-04-01 | |
| Source Publication | MARINE BIOTECHNOLOGY
 |
| ISSN | 1436-2228 |
| Volume | 21Issue:2Pages:217-228 |
| Abstract | Teleost sex differentiation largely depends on the number of undifferentiated germ cells. Here, we describe the generation and characterization of a novel transgenic zebrafish line, Tg(piwil1:egfp-UTRnanos3)(ihb327Tg), which specifically labels the whole lifetime of germ cells, i.e., from primordial germ cells (PGCs) at shield stage to the oogonia and early stage of oocytes in the ovary and to the early stage of spermatogonia, spermatocyte, and spermatid in the testis. By using this transgenic line, we carefully observed the numbers of PGCs from early embryonic stage to juvenile stage and the differentiation process of ovary and testis. The numbers of PGCs became variable at as early as 1day post-fertilization (dpf). Interestingly, the embryos with a high amount of PGCs mainly developed into females and the ones with a low amount of PGCs mainly developed into males. By using transient overexpression and transgenic induction of PGC-specific bucky ball (buc), we further proved that induction of abundant PGCs at embryonic stage promoted later ovary differentiation and female development. Taken together, we generate an ideal transgenic line Tg(piwil1:egfp-UTRnanos3)(ihb327Tg) which can visualize zebrafish germline for a lifetime, and we have utilized this line to study germ cell development and gonad differentiation of teleost and to demonstrate that the increase of PGC number at embryonic stage promotes female differentiation. |
| Keyword | piwil1 Spermatogenesis Oogenesis Primordial germ cell Spermatogonia Oogonia |
| DOI | 10.1007/s10126-019-09874-1 |
| Funding Organization | National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology |
| Indexed By | SCI ; SCI |
| Language | 英语 |
| Funding Project | National Key R&D Program of China[2018YFD0901205] ; National Natural Science Foundation of China[31721005] ; National Natural Science Foundation of China[31872550] ; Strategic Priority Research Program of Chinese Academy of Sciences[XDA08010106] ; State Key Laboratory of Freshwater Ecology and Biotechnology[2016FBZ03] ; State Key Laboratory of Freshwater Ecology and Biotechnology[2015FB11] |
| WOS Research Area | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| WOS Subject | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| WOS ID | WOS:000462935700009 |
| WOS Keyword | SEX-DIFFERENTIATION ; GENE-EXPRESSION ; MAINTENANCE ; TRANSITION ; DIMORPHISM ; TRANSGENE ; MIGRATION ; DEPLETION ; KNOCKOUT ; PATTERN |
| Publisher | SPRINGER |
| Funding Organization | National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/27838 |
| Collection | 鱼类生物学及渔业生物技术研究中心 水生生物多样性与资源保护研究中心 |
| Corresponding Author | Sun, Yonghua |
| Affiliation | 1.Chinese Acad Sci, Innovat Acad Seed Design, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Coll Adv Agr Sci, Beijing 100049, Peoples R China |
| Recommended Citation GB/T 7714 | Ye, Ding,Zhu, Lin,Zhang, Qifeng,et al. Abundance of Early Embryonic Primordial Germ Cells Promotes Zebrafish Female Differentiation as Revealedby Lifetime Labeling of Germline[J]. MARINE BIOTECHNOLOGY,2019,21(2):217-228. |
| APA | Ye, Ding.,Zhu, Lin.,Zhang, Qifeng.,Xiong, Feng.,Wang, Houpeng.,...&Sun, Yonghua.(2019).Abundance of Early Embryonic Primordial Germ Cells Promotes Zebrafish Female Differentiation as Revealedby Lifetime Labeling of Germline.MARINE BIOTECHNOLOGY,21(2),217-228. |
| MLA | Ye, Ding,et al."Abundance of Early Embryonic Primordial Germ Cells Promotes Zebrafish Female Differentiation as Revealedby Lifetime Labeling of Germline".MARINE BIOTECHNOLOGY 21.2(2019):217-228. |
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