IHB OpenIR  > 藻类生物学及应用研究中心
Molecular characterization and toxin quantification of Microcystis panniformis: A microcystin producer in Lake Taihu, China
Gu, Yilu1,2; Gao, Xiaofeng2; Jiang, Yongguang3; Peng, Xin1; Li, Renhui1
Corresponding AuthorLi, Renhui(reli@ihb.ac.cn)
2019-02-01
Source PublicationJOURNAL OF ENVIRONMENTAL SCIENCES-CHINA
ISSN1001-0742
Volume76Pages:359-367
AbstractMicrocystis panniformis is a bloom forming species with flat panniform-like colonies. This species was recently found in Lake Taihu, China. To specifically characterize M. panniformis based on isolated strains, morphological examination on colonial transition and genetic examination are needed. Three M. panniformis strains isolated from a water bloom sample in Lake Taihu were characterized by molecular analysis and toxin quantification. Phylogenetic analysis based on both 16S rRNA gene and internal transcribed spacer (ITS) between 16S and 23S rRNA genes were performed and compared to facilitate easy identification of the species. Relatively high similarities (98%-99%) were shown in 16S rDNA sequences between the strains of M. panniformis and those of other Microcystis species, whereas the similarities for ITS sequences were 88%-95%. In the phylogenetic tree based on the 16S rDNA sequences, the M. panniformis and M. aeruginosa strains were intermixed together with no clear division, whereas all of the M. panniformis strains were clustered together in a single Glade based on the ITS sequences based phylogenyetic tree. The mcyE gene was detected in all three strains, and microcystin was determined by high-performance liquid chromatography. The molecular detection and toxin production of M. panniformis strains are of great significance for the environmental risk assessment of Microcystis blooms. (C) 2018 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V.
KeywordMicrocystis panniformis Blooms ITS of 16S-23S rRNA mcyE Microcystin Taihu
DOI10.1016/j.jes.2018.05.029
Funding OrganizationNational Water Science and Technology Project ; National Water Science and Technology Project ; National Water Science and Technology Project ; National Water Science and Technology Project ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Water Science and Technology Project ; National Water Science and Technology Project ; National Water Science and Technology Project ; National Water Science and Technology Project ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Water Science and Technology Project ; National Water Science and Technology Project ; National Water Science and Technology Project ; National Water Science and Technology Project ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Water Science and Technology Project ; National Water Science and Technology Project ; National Water Science and Technology Project ; National Water Science and Technology Project ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
Indexed BySCI ; SCI
Language英语
Funding ProjectNational Water Science and Technology Project[2017ZX07203-003] ; National Basic Research Program of China[2017YFA0605201] ; National Natural Science Foundation of China[412111020]
WOS Research AreaEnvironmental Sciences & Ecology
WOS SubjectEnvironmental Sciences
WOS IDWOS:000463373300034
WOS KeywordWESENBERGII KOMAREK KOMAREK ; GENETIC DIVERSITY ; WATER-BLOOM ; PHYLOGENETIC-RELATIONSHIPS ; CYANOBACTERIA ; POPULATIONS ; STRAINS ; CULTURE ; 16S ; MORPHOLOGY
PublisherSCIENCE PRESS
Funding OrganizationNational Water Science and Technology Project ; National Water Science and Technology Project ; National Water Science and Technology Project ; National Water Science and Technology Project ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Water Science and Technology Project ; National Water Science and Technology Project ; National Water Science and Technology Project ; National Water Science and Technology Project ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Water Science and Technology Project ; National Water Science and Technology Project ; National Water Science and Technology Project ; National Water Science and Technology Project ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Water Science and Technology Project ; National Water Science and Technology Project ; National Water Science and Technology Project ; National Water Science and Technology Project ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
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Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/27788
Collection藻类生物学及应用研究中心
Corresponding AuthorLi, Renhui
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China
2.Univ Tokyo, Dept Urban Engn, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1138656, Japan
3.China Univ Geosci, Sch Environm Studies, Wuhan 430074, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Gu, Yilu,Gao, Xiaofeng,Jiang, Yongguang,et al. Molecular characterization and toxin quantification of Microcystis panniformis: A microcystin producer in Lake Taihu, China[J]. JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,2019,76:359-367.
APA Gu, Yilu,Gao, Xiaofeng,Jiang, Yongguang,Peng, Xin,&Li, Renhui.(2019).Molecular characterization and toxin quantification of Microcystis panniformis: A microcystin producer in Lake Taihu, China.JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,76,359-367.
MLA Gu, Yilu,et al."Molecular characterization and toxin quantification of Microcystis panniformis: A microcystin producer in Lake Taihu, China".JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA 76(2019):359-367.
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