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Nitrogen-cycling microbial community functional potential and enzyme activities in cultured biofilms with response to inorganic nitrogen availability
Li, Shuangshuang1,2; Peng, Chengrong1; Cheng, Taisheng3; Wang, Chun4,5; Guo, Liangliang1; Li, Dunhai1
Corresponding AuthorLi, Dunhai(lidh@ihb.ac.cn)
2019-02-01
Source PublicationJOURNAL OF ENVIRONMENTAL SCIENCES-CHINA
ISSN1001-0742
Volume76Pages:89-99
AbstractBiofilms mediate crucial biochemical processes in aquatic ecosystems. It was hypothesized that eutrophication may promote the growth of biofilms, resulting in larger numbers of functional genes. However, the metabolic activity and the roles of biofilms in N cycling will be affected by ambient inorganic nitrogen availability, not by the abundance of functional genes. Biofilms were cultured either with replete inorganic nitrogen (N-rep) or without exogenous inorganic nitrogen supply (N-def) in a flow incubator, and the N-cycling gene abundances (nifH, N-2 fixation; amoA, ammonia oxidation, archaea and bacteria; nirS and nirK, denitrification) and enzyme activities (nitrogenase and nitrate reductase) were analyzed. The results showed that, comparing the N-def and N-rep biofilms, the former contained lower nifH gene abundance, but higher nitrogenase activity (NA), while the latter contained higher nifH gene abundance, but lower NA. Different patterns of NA diel variations corresponded to the dynamic microbial community composition and different stages of biofilm colonization. Ammonia oxidizing bacteria (AOB), detected only in N-def biofilms, were responsible for nitrification in biofilms. N-rep biofilms contained high nirS and nirK gene abundance and high denitrification enzyme activity, but N-def biofilms contained significantly lower denitrification gene abundance and activity. In general, the strong N-2 fixation in N-def biofilms and strong denitrification in N-rep biofilms assured the balance of aquatic ecosystems. The results suggested that evaluation of the functional processes of N cycling should not only focus on genetic potential, but also on the physiological activity of biofilms. (C) 2018 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V.
KeywordCultured biofilms Nitrogen availability Nitrogen-cycling enzymes Nitrogen-cycling genes
DOI10.1016/j.jes.2018.03.029
Funding OrganizationMajor Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; Innovation Fund Project of Hebei University of Engineering ; Innovation Fund Project of Hebei University of Engineering ; Innovation Fund Project of Hebei University of Engineering ; Innovation Fund Project of Hebei University of Engineering ; Science and Technology Research and Development Programof Handan ; Science and Technology Research and Development Programof Handan ; Science and Technology Research and Development Programof Handan ; Science and Technology Research and Development Programof Handan ; Major Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; Innovation Fund Project of Hebei University of Engineering ; Innovation Fund Project of Hebei University of Engineering ; Innovation Fund Project of Hebei University of Engineering ; Innovation Fund Project of Hebei University of Engineering ; Science and Technology Research and Development Programof Handan ; Science and Technology Research and Development Programof Handan ; Science and Technology Research and Development Programof Handan ; Science and Technology Research and Development Programof Handan ; Major Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; Innovation Fund Project of Hebei University of Engineering ; Innovation Fund Project of Hebei University of Engineering ; Innovation Fund Project of Hebei University of Engineering ; Innovation Fund Project of Hebei University of Engineering ; Science and Technology Research and Development Programof Handan ; Science and Technology Research and Development Programof Handan ; Science and Technology Research and Development Programof Handan ; Science and Technology Research and Development Programof Handan ; Major Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; Innovation Fund Project of Hebei University of Engineering ; Innovation Fund Project of Hebei University of Engineering ; Innovation Fund Project of Hebei University of Engineering ; Innovation Fund Project of Hebei University of Engineering ; Science and Technology Research and Development Programof Handan ; Science and Technology Research and Development Programof Handan ; Science and Technology Research and Development Programof Handan ; Science and Technology Research and Development Programof Handan
Indexed BySCI ; SCI
Language英语
Funding ProjectMajor Science and Technology Program for Water Pollution Control and Treatment[2017ZX07108-001-01] ; Innovation Fund Project of Hebei University of Engineering[17129033041] ; Science and Technology Research and Development Programof Handan[1723209054-2]
WOS Research AreaEnvironmental Sciences & Ecology
WOS SubjectEnvironmental Sciences
WOS IDWOS:000463373300009
WOS KeywordAMMONIA-OXIDIZING ARCHAEA ; DENITRIFYING BACTERIA ; NIFH EXPRESSION ; FRESH-WATER ; FIXATION ; GENES ; CYANOBACTERIUM ; DIVERSITY ; LAKE ; SEDIMENTS
PublisherSCIENCE PRESS
Funding OrganizationMajor Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; Innovation Fund Project of Hebei University of Engineering ; Innovation Fund Project of Hebei University of Engineering ; Innovation Fund Project of Hebei University of Engineering ; Innovation Fund Project of Hebei University of Engineering ; Science and Technology Research and Development Programof Handan ; Science and Technology Research and Development Programof Handan ; Science and Technology Research and Development Programof Handan ; Science and Technology Research and Development Programof Handan ; Major Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; Innovation Fund Project of Hebei University of Engineering ; Innovation Fund Project of Hebei University of Engineering ; Innovation Fund Project of Hebei University of Engineering ; Innovation Fund Project of Hebei University of Engineering ; Science and Technology Research and Development Programof Handan ; Science and Technology Research and Development Programof Handan ; Science and Technology Research and Development Programof Handan ; Science and Technology Research and Development Programof Handan ; Major Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; Innovation Fund Project of Hebei University of Engineering ; Innovation Fund Project of Hebei University of Engineering ; Innovation Fund Project of Hebei University of Engineering ; Innovation Fund Project of Hebei University of Engineering ; Science and Technology Research and Development Programof Handan ; Science and Technology Research and Development Programof Handan ; Science and Technology Research and Development Programof Handan ; Science and Technology Research and Development Programof Handan ; Major Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; Innovation Fund Project of Hebei University of Engineering ; Innovation Fund Project of Hebei University of Engineering ; Innovation Fund Project of Hebei University of Engineering ; Innovation Fund Project of Hebei University of Engineering ; Science and Technology Research and Development Programof Handan ; Science and Technology Research and Development Programof Handan ; Science and Technology Research and Development Programof Handan ; Science and Technology Research and Development Programof Handan
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/27769
Collection水环境工程研究中心
Corresponding AuthorLi, Dunhai
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China
2.Hebei Univ Engn, Coll Energy & Environm Engn, Hebei Engn Res Ctr Water Pollut Control & Water E, Handan 056038, Peoples R China
3.Natl Univ Tainan, Dept Biol Sci & Technol, Tainan 70005, Taiwan
4.Tsinghua Univ, Sch Environm, Environm Simulat & Pollut Control State Key Joint, Beijing 10084, Peoples R China
5.Tsinghua Univ, Sch Environm, State Environm Protect Key Lab Microorganism Appl, Beijing 10084, Peoples R China
Recommended Citation
GB/T 7714
Li, Shuangshuang,Peng, Chengrong,Cheng, Taisheng,et al. Nitrogen-cycling microbial community functional potential and enzyme activities in cultured biofilms with response to inorganic nitrogen availability[J]. JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,2019,76:89-99.
APA Li, Shuangshuang,Peng, Chengrong,Cheng, Taisheng,Wang, Chun,Guo, Liangliang,&Li, Dunhai.(2019).Nitrogen-cycling microbial community functional potential and enzyme activities in cultured biofilms with response to inorganic nitrogen availability.JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,76,89-99.
MLA Li, Shuangshuang,et al."Nitrogen-cycling microbial community functional potential and enzyme activities in cultured biofilms with response to inorganic nitrogen availability".JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA 76(2019):89-99.
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