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Knockdown of phosphoenolpyruvate carboxykinase increases carbon flux to lipid synthesis in Phaeodactylum tricornutum
Yang, Juan1; Pan, Yufang1; Bowler, Chris2; Zhang, Lingxiang1; Hu, Hanhua1
2016-04-01
Source PublicationALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
ISSN2211-9264
Volume15Pages:50-58
AbstractThe tricarboxylic acid (TCA) cycle is a key metabolic pathway that unifies carbohydrate, lipid and protein metabolism. In Phaeodactylum tricornutum, accumulation of triacylglycerols (TAGs) under nitrogen stress is a consequence of re-allocation of carbon mainly from the intermediates of TCA cycle. Oxaloacetate, an intermediate of the TCA cycle, can flux out of the cycle by the decarboxylation enzyme phosphoenolpyruvate carboxykinase (PEPCK), thus decreasing the carbon flow directed to fatty acid biosynthesis. We assayed the expression of genes encoding mitochondrially-localized decarboxylation enzymes, essential for putative C-4 metabolism, in P. tricornutum. Targeted knockdown of PEPCK was performed using RNA-interference and the photosynthetic characteristics and TAG contents of knockdown lines were characterized. PEPCK was significantly upregulated at both the mRNA and protein levels during TAG accumulation in P. tricornutum, and both PEPCK transcript abundance and enzyme activity were reduced significantly in two RNAi lines. The mutants exhibited lower photosynthetic activity, while TAG content was markedly increased. Meanwhile, cell density was comparable with that of wild-type. We propose that carbon from decarboxylation of C4 metabolites in the mitochondria plays a minor role in carbon concentration in this diatom. Downregulated decarboxylation succeeded in increasing lipid accumulation by decreasing the loss of carbon skeletons without compromising cell biomass. (C) 2016 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordPhaeodactylum Tricornutum Triacylglycerol Phosphoenolpyruvate Carboxykinase Alga Biofuel Rnai
DOI10.1016/j.algal.2016.02.004
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNational Natural Science Foundation of China(41576144) ; National Natural Science Foundation of China(41576144) ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2015FB12) ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2015FB12) ; ANR DiaDomOil project ; ANR DiaDomOil project ; ERC Advanced Award Diatomite ; ERC Advanced Award Diatomite ; National Natural Science Foundation of China(41576144) ; National Natural Science Foundation of China(41576144) ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2015FB12) ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2015FB12) ; ANR DiaDomOil project ; ANR DiaDomOil project ; ERC Advanced Award Diatomite ; ERC Advanced Award Diatomite
Language英语
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS KeywordDIATOM FISTULIFERA-SOLARIS ; MARINE DIATOM ; THALASSIOSIRA-PSEUDONANA ; OIL ACCUMULATION ; METABOLISM ; GENOME ; NITROGEN ; LOCALIZATION ; ALGAE ; PHYTOPLANKTON
WOS IDWOS:000372452500007
Funding OrganizationNational Natural Science Foundation of China(41576144) ; National Natural Science Foundation of China(41576144) ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2015FB12) ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2015FB12) ; ANR DiaDomOil project ; ANR DiaDomOil project ; ERC Advanced Award Diatomite ; ERC Advanced Award Diatomite ; National Natural Science Foundation of China(41576144) ; National Natural Science Foundation of China(41576144) ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2015FB12) ; Open Fund Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2015FB12) ; ANR DiaDomOil project ; ANR DiaDomOil project ; ERC Advanced Award Diatomite ; ERC Advanced Award Diatomite
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/27758
Collection藻类生物学及应用研究中心
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
2.PSL Res Univ, Ecole Normale Super, Inst Biol, CNRS,INSERM,U1024,UMR 8197, 46 Rue Ulm, F-75005 Paris, France
Recommended Citation
GB/T 7714
Yang, Juan,Pan, Yufang,Bowler, Chris,et al. Knockdown of phosphoenolpyruvate carboxykinase increases carbon flux to lipid synthesis in Phaeodactylum tricornutum[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2016,15:50-58.
APA Yang, Juan,Pan, Yufang,Bowler, Chris,Zhang, Lingxiang,&Hu, Hanhua.(2016).Knockdown of phosphoenolpyruvate carboxykinase increases carbon flux to lipid synthesis in Phaeodactylum tricornutum.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,15,50-58.
MLA Yang, Juan,et al."Knockdown of phosphoenolpyruvate carboxykinase increases carbon flux to lipid synthesis in Phaeodactylum tricornutum".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 15(2016):50-58.
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