IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心
Fish reovirus GCReV-109 VP33 protein elicits protective immunity in rare minnows
Liu, Jia; Pei, Chao; Gao, Xiao-chan; Chen, Zhong-yuan; Zhang, Qi-Ya
2016-03-01
Source PublicationARCHIVES OF VIROLOGY
ISSN0304-8608
Volume161Issue:3Pages:573-582
AbstractGrass carp reovirus strain 109 (GCReV-109) was previously isolated from a grass carp (Ctenopharyngodon idellus) with hemorrhagic disease, and its complete genome has been sequenced. However, the infectivity of GCReV-109 has not been studied, and the viral protein VP33, encoded on genome segment S11, had no detectable sequence homology to other known reovirus proteins. In this study, we characterized GCReV-109 infections in vivo and in vitro, as well as the VP33 protein. Infectivity analysis showed that GCReV-109 caused severe hemorrhagic disease and 100 % mortality at dilutions up to 10(-4) in rare minnows (Gobiocypris rarus) by 8 days postinfection, but no visible cytopathic effect was observed in GCReV-109-infected subcultured grass carp muscle (GCM) cells. To confirm that GCReV-109 could be propagated in GCM cells, three virus genome segments were detected by RT-PCR, and large numbers of virus particles were observed by transmission electron microscopy in samples from the infected GCM cells. The suspension of GCReV-109-infected GCM cells was pathogenic to rare minnows. VP33 protein was expressed and purified for generation of an anti-VP33 antiserum. In western blot analysis of purified GCReV-109 particles, the antiserum specifically recognized a protein band (approximately 33 kDa). This revealed that VP33 is a major structural protein of GCReV-109 that might have immunogenic properties. The protective efficacy of the anti-VP33 antiserum against GCReV-109 infection was tested. The death of infected fish was delayed and the mortality fell to 10 % when fish were treated with the anti-VP33 antiserum, suggesting that it might be useful for the prevention and control of fish reoviral disease.
SubtypeArticle
DOI10.1007/s00705-015-2675-9
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNational Natural Science Foundation of China(31430091) ; National Natural Science Foundation of China(31430091) ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2011FBZ12) ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2011FBZ12) ; National Natural Science Foundation of China(31430091) ; National Natural Science Foundation of China(31430091) ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2011FBZ12) ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2011FBZ12)
Language英语
WOS Research AreaVirology
WOS SubjectVirology
WOS KeywordGRASS CARP REOVIRUS ; SPOT SYNDROME VIRUS ; COMPLETE GENOME SEQUENCE ; PROTEOMIC ANALYSIS ; MONOCLONAL-ANTIBODIES ; START SITE ; CELL-LINE ; CHINA ; IDENTIFICATION ; AQUAREOVIRUS
WOS IDWOS:000371049800008
Funding OrganizationNational Natural Science Foundation of China(31430091) ; National Natural Science Foundation of China(31430091) ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2011FBZ12) ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2011FBZ12) ; National Natural Science Foundation of China(31430091) ; National Natural Science Foundation of China(31430091) ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2011FBZ12) ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology(2011FBZ12)
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/27734
Collection鱼类生物学及渔业生物技术研究中心
AffiliationUniv Chinese Acad Sci, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Liu, Jia,Pei, Chao,Gao, Xiao-chan,et al. Fish reovirus GCReV-109 VP33 protein elicits protective immunity in rare minnows[J]. ARCHIVES OF VIROLOGY,2016,161(3):573-582.
APA Liu, Jia,Pei, Chao,Gao, Xiao-chan,Chen, Zhong-yuan,&Zhang, Qi-Ya.(2016).Fish reovirus GCReV-109 VP33 protein elicits protective immunity in rare minnows.ARCHIVES OF VIROLOGY,161(3),573-582.
MLA Liu, Jia,et al."Fish reovirus GCReV-109 VP33 protein elicits protective immunity in rare minnows".ARCHIVES OF VIROLOGY 161.3(2016):573-582.
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