IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心
The Edwardsiella piscicida Type III Translocon Protein EseC Inhibits Biofilm Formation by Sequestering EseE
Liu, Ying Li1,2,3; He, Tian Tian1,2,3; Liu, Lu Yi1,2; Yi, Jia1,2,3; Nie, Pin1,2,4,5; Yu, Hong Bing6,7; Xie, Hai Xia1,2
Corresponding AuthorXie, Hai Xia(xieh@ihb.ac.cn)
2019-04-01
Source PublicationAPPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN0099-2240
Volume85Issue:8Pages:11
AbstractThe type III secretion system (T3SS) is one of the most important virulence factors of the fish pathogen Edwardsiella piscicida. It contains three translocon proteins, EseB, EseC, and EseD, required for translocation of effector proteins into host cells. We have previously shown that EseB forms filamentous appendages on the surface of E. piscicida, and these filamentous structures mediate bacterial cell-cell interactions promoting autoaggregation and biofilm formation. In the present study, we show that EseC, but not EseD, inhibits the autoaggregation and biofilm formation of E. piscicida. At 18 h postsubculture, a Delta eseC strain developed strong autoaggregation and mature biofilm formation, accompanied by enhanced formation of EseB filamentous appendages. This is in contrast to the weak autoaggregation and immature biofilm formation seen in the E. piscicida wild-type strain. EseE, a protein that directly binds to EseC and also positively regulates the transcription of the escC-eseE operon, was liberated and showed increased levels in the absence of EseC. This led to augmented transcription of the escC-eseE operon, thereby increasing the steady-state protein levels of intracellular EseB, EseD, and EseE, as well as biofilm formation. Notably, the levels of intracellular EseB and EseD produced by the Delta eseE and Delta eseC Delta eseE strains were similar but remarkably lower than those produced by the wild-type strain at 18 h postsubculture. Taken together, we have shown that the translocon protein EseC inhibits biofilm formation through sequestering EseE, a positive regulator of the escC-eseE operon. IMPORTANCE Edwardsiella piscicida, previously known as Edwardsiella tarda, is a Gram-negative intracellular pathogen that mainly infects fish. The type III secretion system (T3SS) plays a pivotal role in its pathogenesis. The T3SS translocon protein EseB is required for the assembly of filamentous appendages on the surface of E. piscicida. The interactions between the appendages facilitate autoaggregation and biofilm formation. In this study, we explored the role of the other two translocon proteins, EseC and EseD, in biofilm formation. We have demonstrated that EseC, but not EseD, inhibits the autoaggregation and biofilm formation of E. piscicida, providing new insights into the regulatory mechanism involved in E. piscicida biofilm formation.
KeywordEdwardsiella piscicida biofilm translocon protein type III secretion system
DOI10.1128/AEM.02133-18
Funding OrganizationNational Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology
Indexed BySCI ; SCI
Language英语
Funding ProjectNational Natural Science Foundation of China (NSFC)[31572659] ; China Agriculture Research System[CARS-46] ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology[2016FBZ04]
WOS Research AreaBiotechnology & Applied Microbiology ; Microbiology
WOS SubjectBiotechnology & Applied Microbiology ; Microbiology
WOS IDWOS:000463407200006
WOS KeywordSECRETION SYSTEM ; TARDA ; PATHOGENESIS ; DISSECTION ; EXPRESSION ; INFECTION ; VIRULENCE ; CHAPERONE ; EFFECTOR ; GENES
PublisherAMER SOC MICROBIOLOGY
Funding OrganizationNational Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/27732
Collection鱼类生物学及渔业生物技术研究中心
Corresponding AuthorXie, Hai Xia
Affiliation1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Minist Agr, Inst Hydrobiol, Wuhan, Hubei, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Minist Agr, Key Lab Aquaculture Dis Control, Wuhan, Hubei, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China
5.Qingdao Agr Univ, Coll Marine Sci & Engn, Qingdao, Shandong, Peoples R China
6.Univ British Columbia, BC Childrens Hosp Res Inst, Dept Pediat, Vancouver, BC, Canada
7.Univ British Columbia, Vancouver, BC, Canada
Recommended Citation
GB/T 7714
Liu, Ying Li,He, Tian Tian,Liu, Lu Yi,et al. The Edwardsiella piscicida Type III Translocon Protein EseC Inhibits Biofilm Formation by Sequestering EseE[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2019,85(8):11.
APA Liu, Ying Li.,He, Tian Tian.,Liu, Lu Yi.,Yi, Jia.,Nie, Pin.,...&Xie, Hai Xia.(2019).The Edwardsiella piscicida Type III Translocon Protein EseC Inhibits Biofilm Formation by Sequestering EseE.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,85(8),11.
MLA Liu, Ying Li,et al."The Edwardsiella piscicida Type III Translocon Protein EseC Inhibits Biofilm Formation by Sequestering EseE".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 85.8(2019):11.
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