IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心
Transcriptome analysis of soiny mullet (Liza haematocheila) spleen in response to Streptococcus dysgalactiae
Qi, Zhitao1; Wu, Ping2; Zhang, Qihuan1; Wei, Youchuan3; Wang, Zisheng1; Qiu, Ming1; Shao, Rong1; Li, Yao1; Gao, Qian4
2016-02-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume49Pages:194-204
AbstractSoiny mullet (Liza haematocheila) is becoming an economically important aquaculture mugilid species in China and other Asian countries. However, increasing incidences of bacterial pathogenic diseases has greatly hampered the production of the soiny mullet. Deeper understanding of the soiny mullet immune system and its related genes in response to bacterial infections are necessary for disease control in this species. In this study, the transcriptomic profile of spleen from soiny mullet challenged with Streptococcus dysgalactiae was analyzed by Illumina-based paired-end sequencing method. After assembly, 86,884 unique transcript fragments (unigenes) were assembled, with an average length of 991 bp. Approximately 41,795 (48.1%) unigenes were annotated in the nr NCBI database and 57.9% of the unigenes were similar to that of the Nile tilapia. A total of 24,299 unigenes were categorized into three Gene Ontology (GO) categories (molecular function, cellular component and biological process), 13,570 unigenes into 25 functional Clusters of Orthologous Groups of proteins (COG) categories, and 30,547 unigenes were grouped into 258 known pathways in the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. Following S. dysgalactiae infection, 11,461 differentially expressed unigenes were identified including 4658 up-regulated unigenes and 6803 down-regulated unigenes. Significant enrichment analysis of these differentially expressed unigenes identified major immune related pathways, including the Toll-like receptor, complement and coagulation cascades, T cell receptor signaling pathway and B cell receptor signaling pathway. In addition, 24,813 simple sequence repeats (SSRs) and 127,503 candidate single nucleotide polymorphisms (SNPs) were identified from the mullet spleen transcriptome. To this date, this study has globally analyzed the transcriptome profile from the spleen of L. haematocheila after S. dysgalactiae infection. Therefore, the results of our study contributes to better on the immune system and defense mechanisms of soiny mullet in response to bacterial infection, and provides valuable references for related studies in mugilidae species which currently lack genomic reference. (C) 2015 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordLiza Haematocheila Streptococcus Dysgalactiae Spleen Transcriptome Infection Immune Mechanism
DOI10.1016/j.fsi.2015.12.029
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNational Natural Science Foundation of China(31302221 ; National Natural Science Foundation of China(31302221 ; Jiangsu Province(BK2011418 ; Jiangsu Province(BK2011418 ; opening research fund of the Jiangsu Key Laboratory of Biochemistry and Biotechnology of Marine Wetland ; opening research fund of the Jiangsu Key Laboratory of Biochemistry and Biotechnology of Marine Wetland ; "Qinglan" project of Jiangsu province ; "Qinglan" project of Jiangsu province ; 31272666) ; 31272666) ; BK20151297) ; BK20151297) ; National Natural Science Foundation of China(31302221 ; National Natural Science Foundation of China(31302221 ; Jiangsu Province(BK2011418 ; Jiangsu Province(BK2011418 ; opening research fund of the Jiangsu Key Laboratory of Biochemistry and Biotechnology of Marine Wetland ; opening research fund of the Jiangsu Key Laboratory of Biochemistry and Biotechnology of Marine Wetland ; "Qinglan" project of Jiangsu province ; "Qinglan" project of Jiangsu province ; 31272666) ; 31272666) ; BK20151297) ; BK20151297)
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS KeywordCARP CYPRINUS-CARPIO ; AEROMONAS-HYDROPHILA ; VIBRIO-ANGUILLARUM ; INFLAMMATORY CELLS ; COMPLEMENT-SYSTEM ; IMMUNE-RESPONSE ; RAINBOW-TROUT ; RNA-SEQ ; FISH ; GENES
WOS IDWOS:000370884200020
Funding OrganizationNational Natural Science Foundation of China(31302221 ; National Natural Science Foundation of China(31302221 ; Jiangsu Province(BK2011418 ; Jiangsu Province(BK2011418 ; opening research fund of the Jiangsu Key Laboratory of Biochemistry and Biotechnology of Marine Wetland ; opening research fund of the Jiangsu Key Laboratory of Biochemistry and Biotechnology of Marine Wetland ; "Qinglan" project of Jiangsu province ; "Qinglan" project of Jiangsu province ; 31272666) ; 31272666) ; BK20151297) ; BK20151297) ; National Natural Science Foundation of China(31302221 ; National Natural Science Foundation of China(31302221 ; Jiangsu Province(BK2011418 ; Jiangsu Province(BK2011418 ; opening research fund of the Jiangsu Key Laboratory of Biochemistry and Biotechnology of Marine Wetland ; opening research fund of the Jiangsu Key Laboratory of Biochemistry and Biotechnology of Marine Wetland ; "Qinglan" project of Jiangsu province ; "Qinglan" project of Jiangsu province ; 31272666) ; 31272666) ; BK20151297) ; BK20151297)
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/27727
Collection鱼类生物学及渔业生物技术研究中心
Affiliation1.Yancheng Inst Technol, Dept Ocean Technol, Key Lab Aquaculture & Ecol Coastal Pool Jiangsu P, 211 Eastern Jianjun Rd, Yancheng 224051, Jiangsu, Peoples R China
2.Hubei Univ Sci & Technol, Hubei Prov Key Lab Cardiovasc Cerebrovasc & Metab, Xianning 437100, Hubei, Peoples R China
3.Guangxi Univ, Guangxi Key Lab Subtrop Bioresource Conservat & U, Nanning 530004, Guangxi Autonom, Peoples R China
4.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, 7 South Donghu Rd, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Qi, Zhitao,Wu, Ping,Zhang, Qihuan,et al. Transcriptome analysis of soiny mullet (Liza haematocheila) spleen in response to Streptococcus dysgalactiae[J]. FISH & SHELLFISH IMMUNOLOGY,2016,49:194-204.
APA Qi, Zhitao.,Wu, Ping.,Zhang, Qihuan.,Wei, Youchuan.,Wang, Zisheng.,...&Gao, Qian.(2016).Transcriptome analysis of soiny mullet (Liza haematocheila) spleen in response to Streptococcus dysgalactiae.FISH & SHELLFISH IMMUNOLOGY,49,194-204.
MLA Qi, Zhitao,et al."Transcriptome analysis of soiny mullet (Liza haematocheila) spleen in response to Streptococcus dysgalactiae".FISH & SHELLFISH IMMUNOLOGY 49(2016):194-204.
Files in This Item:
File Name/Size DocType Version Access License
1-s2.0-S105046481530(2231KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Qi, Zhitao]'s Articles
[Wu, Ping]'s Articles
[Zhang, Qihuan]'s Articles
Baidu academic
Similar articles in Baidu academic
[Qi, Zhitao]'s Articles
[Wu, Ping]'s Articles
[Zhang, Qihuan]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Qi, Zhitao]'s Articles
[Wu, Ping]'s Articles
[Zhang, Qihuan]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 1-s2.0-S1050464815302874-main.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.